Cholera toxin
Oral Immunization with Cholera Toxin Provides Protection against Campylobacter jejuni in an Adult Mouse Intestinal Colonization Model
... to cholera toxin (CT) reacted with the major outer membrane proteins (MOMPs) from different strains of ...the toxin (CT-B), which is nontoxic and a component of licensed oral cholera vaccines, ...
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Adjuvant Activity of the Catalytic A1 Domain of Cholera Toxin for Retroviral Antigens Delivered by GeneGun
... of cholera toxin (CTA1) for human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) antigens in mice and macaques delivered by ...
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A Cholera Toxin B-subunit Variant That Binds Ganglioside G M1 but Fails to Induce Toxicity
... of Toxin to Non-polarized T84 Cells—Binding isotherms for wild-type CTB and mutant CTB-H57A were determined by incubating at 4 °C increasing amount of toxins in HBSS and ...the cholera toxin ...
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Cell propagation of Cholera Toxin CTA ADP-ribosylating factor by exosome mediated transfer.
... well plates containing coverslips until 60-70% of confluency. Cells were incubated at 37 °C with or without 7 µM BODIPY C16 for 4 h. 12 nM CT was added for 20 min on ice (T0), and cells were then incubated at 37 °C for ...
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Cholera toxin promotes the induction of regulatory T cells by modulation dendritic cell activation
... Th1 or Th2 cells specific for foreign Ag can be induced using adjuvants based on pathogen-derived molecules that promote reg- ulatory cytokine production and dendritic cell (DC) maturation through binding to pathogen ...
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In vitro and in vivo cholera toxin production by classical and El Tor isolates of Vibrio cholerae
... A comparative study was carried out on the in vitro production of cholera toxin by 19 Vibrio cholerae El Tor isolates from patients with cholera in South Africa, one El Tor isolate from [r] ...
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Designing, Optimization and Construction of Myelin Basic Protein Coding Sequence Binding to the Immunogenic Subunit of Cholera Toxin
... Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the intensity of ...
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Localization of the action of cholera toxin on adenyl cyclase in mucosal epithelial cells of rabbit intestine
... the toxin. Cholera toxin, which makes contact with the luminal side of the epithelial cells, in the natural disease and in the experimental model, would appear to exert its pathologic effect on ...
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Role of platelet activating factor in Chinese hamster ovary cell responses to cholera toxin
... of cholera toxin on eukaryotic cells may lead to improved understanding of cholera toxin ef- fects on cytoskeleton and intestinal secretion and potentially to new approaches to antisecretory ...
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Effects of cholera toxin on innate and adaptive immunity and its application as an immunomodulatory agent
... Abstract: Cholera toxin (CT) is a potent vaccine adjuvant when administered via parenteral, muco- sal, or transcutaneous routes. It also inhibits innate inflammatory responses induced by pathogen-de- rived ...
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Intestinal Secretion Induced by Vasoactive Intestinal Polypeptide: A COMPARISON WITH CHOLERA TOXIN IN THE CANINE JEJUNUM IN VIVO
... The effect of vasoactive intestinal polypeptide (VIP) on intestinal water and electrolyte transport and transmucosal potential difference was investigated in the dog jejunum in vivo and compared to secretion induced by ...
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Automatic and manual latex agglutination tests for measurement of cholera toxin and heat labile enterotoxin of Escherichia coli
... M glycine-NaOH buffer This paper deals with automatic and manual Dow latex suspension inM0.05 8.2 containing 0.05 sodium chloride was mixed latex agglutination tests for cholera toxin CT[r] ...
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Quantitative Detection of Vibrio cholera Toxin by Real Time and Dynamic Cytotoxicity Monitoring
... fore, development of a laboratory tool to detect and to identify CT quickly and accurately is desirable for controlling and preventing V. cholerae-related epidemic diseases (43, 44). The CT-RTCA sys- tem had a ...
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Thiazolidinone CFTR inhibitor identified by high throughput screening blocks cholera toxin–induced intestinal fluid secretion
... the inadequacy of available inhibitors as lead com- pounds, we screened a collection of diverse druglike compounds using a cell-based fluorescence assay devel- oped in our laboratory (12–15). The high-throughput ...
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A Single Native Ganglioside GM1 Binding Site Is Sufficient for Cholera Toxin To Bind to Cells and Complete the Intoxication Pathway
... Figure 1 shows the purification process for the AMBT strain (mutant CTB-G33D, wt CTB tagged). Since CTB pentamers nat- urally bind to metal affinity resins (25), cholera toxin variants can be purified to ...
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Kinetics of Local and Systemic Immune Responses after Vaginal Immunization with Recombinant Cholera Toxin B Subunit in Humans
... recombinant cholera toxin B subunit (CTB) in generating mucosal and systemic immune responses in healthy women were evaluated, and the kinetics of the immune responses were monitored for responding ...
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Cholera toxin inhibits signal transduction by several mitogens and the in vitro growth of human small cell lung cancer
... Cholera toxin (CT) inhibited the in vitro growth of three of four human small-cell lung carcinoma (SCLC) cell lines with a 50% inhibitory concentration of 27-242 ...
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Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin 1 or 2
... Cholera toxin (CT) causes the massive secretory diarrhea associated with epidemic cholera. To induce disease, CT enters the cytosol of host cells by co-opting a lipid-based sorting pathway from the ...
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A Single Native Ganglioside GM1-Binding Site Is Sufficient for Cholera Toxin to Bind to Cells and Complete the Intoxication Pathway
... Figure 1 shows the purification process for the AMBT strain (mutant CTB-G33D, wt CTB tagged). Since CTB pentamers nat- urally bind to metal affinity resins (25), cholera toxin variants can be purified to ...
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Escherichia coli K88ac Fimbriae Expressing Heat-Labile and Heat-Stable (STa) Toxin Epitopes Elicit Antibodies That Neutralize Cholera Toxin and STa Toxin and Inhibit Adherence of K88ac Fimbrial E. coli
... eliminate toxin activity would provide broad-spectrum protection against ...LT toxin (LTP1, 8 LCSEYRNTQIYTIN 21 ) and an STa toxoid epitope ( 5 CCELCCNPQCAGCY 18 ) were em- bedded in the FaeG major subunit ...
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