Cholera toxin-b
Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines
... Figure 1 Phase-contrast microscope images of rT4 and T24 cells treated with either cholera toxin B (cTB) or temperature. Images of rT4 A) and T24 B) cells growing in normal conditions without ...
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A Cholera Toxin B-subunit Variant That Binds Ganglioside G M1 but Fails to Induce Toxicity
... of Toxin to Non-polarized T84 Cells—Binding isotherms for wild-type CTB and mutant CTB-H57A were determined by incubating at 4 °C increasing amount of toxins in HBSS and ...the cholera toxin ...
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Kinetics of Local and Systemic Immune Responses after Vaginal Immunization with Recombinant Cholera Toxin B Subunit in Humans
... recombinant cholera toxin B subunit (CTB) in generating mucosal and systemic immune responses in healthy women were evaluated, and the kinetics of the immune responses were monitored for responding ...
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Anti-inflammatory effect of cholera toxin B subunit in experimental stroke
... Cholera toxin is composed of one A subunit and five B ...The cholera toxin B subunit (CTB) is devoid of toxicity and capable of ...
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Comparison of Serum Humoral Responses Induced by Oral Immunization with the Hepatitis B Virus Core Antigen and the Cholera Toxin B Subunit
... hepatitis B virus core (HBc) virus-like particle (VLP) is known as one of the most immunogenic antigens and carrier vehicles in different immunization ...recombinant cholera toxin B pentamer, ...
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Cholera Toxin B-Subunit Gene Enhances Mucosal Immunoglobulin A, Th1-Type, and CD8+ Cytotoxic Responses When Coadministered Intradermally with a DNA Vaccine
... The cell pellets were diluted 1:1 in double-strength sample buffer 120 mM Tris [pH 6.8], 0.1% sodium dodecyl sulfate, 10% glycerol, 1% bromophenol blue, 100 mM dithiothreitol ([DTT]) and boiled for 3 min. Samples along ...
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A protein-based pentavalent inhibitor of the cholera toxin B-subunit.
... Here we report a multivalent inhibitor for cholera toxin, based on an inactive mutant CTB protein that is modified with GM1os ligands (Figure 1). As the majority of the structure comes as a ready-made ...
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Gas phase characterization of the noncovalent quaternary structure of Cholera toxin and the Cholera toxin B subunit pentamer
... CTx B pentamer, the additional interactions present in the CTx B helical region (residues 1–14) may provide the increase in structural stability observed in both the gas and aqueous ...different B 5 ...
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Designing, Optimization and Construction of Myelin Basic Protein Coding Sequence Binding to the Immunogenic Subunit of Cholera Toxin
... Background and Objectives: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease. Mucosal feeding of myelin basic protein binding to the cholera toxin B subunit can reduce the ...
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Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury
... APP: Amyloid precursor protein; BBB: Blood brain barrier; CTB: Cholera toxin B; DAB: 3,3 ′ -diaminobenzidine; DAPI: 4 ′ , 6-diamidino-2-phenylindole; DLK: Dual leucine zipper kinase (MAP[r] ...
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Effects of cholera toxin on adenylate cyclase Studies with guanylylimidodiphosphate
... of cholera toxin and Gpp(NH)p on adenylate cyclase was ...by cholera toxin in vivo, or by cholera toxin and NAD in homogenates, blocked the stimulatory effect of ...
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Rotavirus 2/6 Virus-Like Particles Administered Intranasally in Mice, with or without the Mucosal Adjuvants Cholera Toxin and Escherichia coli Heat-Labile Toxin, Induce a Th1/Th2-Like Immune Response
... adjuvants cholera toxin and Escherichia coli heat-labile toxin (LT) consid- erably enhanced the Th1/Th2-like response; notably, significant levels of IL-2, IL-4, and IL-5 were ...
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Lipid Membrane Based Biosensors
... potentiometric cholera toxin minisensor on graphene nanosheets with incorporated lipid films has been described in the literature ...natural cholera toxin receptor) was incorporated on ...
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In vitro and in vivo cholera toxin production by classical and El Tor isolates of Vibrio cholerae
... A comparative study was carried out on the in vitro production of cholera toxin by 19 Vibrio cholerae El Tor isolates from patients with cholera in South Africa, one El Tor isolate from [r] ...
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Neutralization of cholera toxin by Rosaceae family plant extracts
... C688 cells were incubated for 1 h with CTB-FITC; next they were fixed to glass slides and observed under a fluorescence microscope. In cells treated only with CTB- FITC, the CTB-FITC accumulated in the perinuclear region ...
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Protective Mucosal Immunity to Ocular Herpes Simplex Virus Type 1 Infection in Mice by Using Escherichia coli Heat-Labile Enterotoxin B Subunit as an Adjuvant
... gens mixed with the mucosal adjuvant comprising both cholera toxin (Ctx) and its B subunit (CtxB) (27). A strong systemic and mucosal immune response to HSV-1 was induced with high levels of serum ...
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Long-term neuronal survival, regeneration, and transient target reconnection after optic nerve crush and mesenchymal stem cell transplantation
... labeled with TOPRO-3 (blue). Scale bar: 50 μ m. SC: superior colliculus. Figure S4. Visual behaviors analysis. After 63 days of left-nerve crush, animals were submitted to axotomy of the right nerve and tested for visual ...
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Sequence of Events Mediating the Effect of Cholera Toxin on Rat Thymocytes
... We have found that in rat thymocytes binding of [ 125 I]choleragen is followed by cellular accumulation of cyclic 3¢,5¢-AMP which, in turn, is followed by stimulation of amino acid transport. Binding of cholera ...
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Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii
... Large scale recombinant plasmid DNA was prepared by the alkaline lysis method. Plasmids were diluted and suspended in sterile phosphate buffered saline (PBS) to a final concentration of 1 μg/μl. BALB/c mice were ran- ...
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Treatments of cholera, their restraints and the necessity for simple prevention strategies a comprehensive review
... is Cholera, exclusively caused by the bacterium Vibrio cholerae, a gram negative, rod shaped, and facultative ...of cholera since 1817 emerging from Ganges delta of Indian Subcontinent spreading still ...
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