Cloning and Identification
FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.
... 2 0022-538X/82/110674-09$02.00/0 Copyright © 1982, American Society for Microbiology FBJ Murine Osteosarcoma Virus: Identification and Molecular Cloning of Biologically Active Proviral D[r] ...
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Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene
... We identified twenty five cellulase positive BAC clones (inluding nine β-glucosidases). The high number of hydrolytic clones is consistent with the adaptation of the rumen microbial community for the digestion of plant ...
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Cloning and identification of human Sca as a novel inhibitor of osteoclast formation and bone resorption
... In summary, we have identified a novel inhibitor of osteo- clast activity, OIP-1, that is released as a membrane-bound GPI-linked protein. OIP-I is a member of the human Ly6 fam- ily of proteins, and our findings ...
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Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing
... Reports on multidrug-resistant M. tuberculosis infections are increasing (28), and most of the rifampin-resistant strains are also resistant to isoniazid. Therefore, rifampin resistance as a surro- gate marker of ...
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Use of monoclonal antibodies to facilitate identification, cloning, and purification of Chlamydia trachomatis hsp10
... cell proteins (Fig. 3B). Recognition of an unidentified 45-kDa protein in both HeLa cells and chlamydiae was common among several of the cross-reactive antibodies. A BLAST search of GenBank with the MAP2 immunogen ...
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Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest
... genome thus facilitating subsequent recovery of the plasmid from the mammalian cells. The cells were rinsed extensively to remove untransfected plasmid and dead or dying cells. The cultures were then treated with ...
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Glucoamylase of Caulobacter crescentus CB15: cloning and expression in Escherichia coli and functional identification
... The deduced amino acid sequence of the cc2282 gene showed 47% similarity to that of both TtGA and Clostridium sp. G0005 GA (CGA, ClostGA), and the gene is registered in the CAZy database as CcGA (CauloGA) which belongs ...
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Squalene synthase cloning and functional identification in wintersweet plant (Chimonanthus zhejiangensis)
... Cloning of squalene synthase genes from wintersweet The transcriptome of the C. zhejiangensis foliage was sequenced by Illumina. The data was assembled by the Trinity software (Grabherr et al. 2011) and the result ...
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Identification, Characterization, and Phylogenetic analysis of Pigeon pea (Cajanus cajan L. Mill sp.) Resistance Gene Analogs using PCR cloning and in silico methods
... Several single nucleotide polymorphisms (SNPs) were found when nucleotide sequences were aligned. These nucleotide variations will be helpful in developing cleaved amplified polymorphic markers (CAPS) that can be used ...
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The construction and characteristics of a BAC library for Cucumis sativus L. ‘B10’
... Abstract: Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene ...
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Identification, Molecular Cloning, and Analysis of Full Length Hepatitis C Virus Transmitted/Founder Genotypes 1, 3, and 4
... Previously, we described a conceptual and mathematical model of early HCV sequence diversification that allowed for unambiguous identification of T/F genomes based on an analysis of quarter- or half-genome ...
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Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.
... 714 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest DNA sequence analysis of part of the human herpesvirus 6 HHV-6 genome led to the identification of an open reading f[r] ...
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Identification and cloning of GOLDEN2-LIKE1 (GLK1), a transcription factor associated with chloroplast development in Brassica napus L.
... In this study, we identified the GOLDEN2-LIKE transcription factor (GLK1) in B. napus which may be associated with chloroplast development and located in a Quantitative Trait Loci region for leaf photosynthesis rate and ...
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The recognizing of fli C gene in Pseudomonas aeruginosa isolated from clinical sample with PCR
... 9-Brimer C.D. Montie T.C., Cloning and Comparison of fliC Genes and Identification of Glycosylation in the Flagellin of Pseudomonas aeruginosa a-Type Strains. Journal of Bacteriology, 1998. 180(12) ; ...
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Polymorphism of SCD1 and DGAT1 Gene in Isfahan Holstein Cows
... [8] Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P, Spelman R, Georges M, Snell R. Positional candidate cloning of a QTL in dairy cattle: Identification of ...
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Investigating the Protective Effects of Telomerase Reverse Transcriptase on Neuronal Metabolism and Resistance to Amyloid-beta
... Cloning of Human Telomerase Catalytic Subunit (hTERT) Gene Promoter and Identification of Proximal Core Promoter Sequences Essential for Transcriptional Activation in Immortalized and [r] ...
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Effect of Plasma-leptin on Cardiac Disorders with Type 2 Diabetes-patients
... Author has declared that no competing interests exist. Leptin, its receptor and obesity. Identification and expression cloning of a leptin receptor, OB-R. Leptin gene[r] ...
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Advances with RNA interference in Alzheimer’s disease research
... A. Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein ...
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A new family of proteins (rBAT and 4F2hc) involved in cationic and zwitterionic amino acid transport: a tale of two proteins in search of a transport function
... The proteins rBAT and 4F2hc introduce intriguing questions regarding amino acid transport. (1) How do these two proteins, which apparently do not act as pore-forming proteins, participate in specific amino acid transport ...
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Genetic epidemiology of diabetes
... positional cloning, and candidate gene studies have been most successful in the forms of diabetes with the sim- plest genetic ...positional cloning and studies of positional candidate genes led to the ...
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