Cloning and protein expression system
Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... To determine the immunogenicity of the baculovirus expressed VP4, rabbits were immunized with the VP4 lysate as described in Methods. The ability of rabbit antiserum to recognize VP4 in intact SA11 was investigated by ...
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alicator Ligation Independent Cloning and Expression System
... target protein. Following protein affinity purification, amino-terminal tags can be removed either via enterokinase (EK), DDDDK^ or a novel protease WELQut (WQ), ...
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Cloning, expression and characterization of gE protein of Duck plague virus
... Conclusions In conclusion, the DPV gE gene has been successfully expressed in a prokaryotic expression system, and we present the basic characteristics of DPV gE product. The immunofluorescence studies ...
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Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli
... this protein facilitates the binding of antibody molecules to VP1, and hence VP1 can provoke the immune system of the body ...pET expression vector and the protein was over expressed in ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... DISCUSSION Over 50% of the Wolbachia pipientis wMel genome encodes proteins with at least two cysteines, and many of these would be expected to form disulfides. This situation suggests that a disulfide pathway must be ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried ...
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Cloning and developmental expression of zebrafish pdzrn3
... PDZRN/SEMCAP/LNX protein family containing a RING finger and two PDZ domains, has been implicated in myoblast and osteoblast ...spatio-temporal expression pattern during embryonic development has not been ...
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Cloning and Expression of a DNA Sequence Encoding a 41-Kilodalton Cryptosporidium parvum Oocyst Wall Protein
... Immunoscreening of C. parvum genomic DNA libraries for CP41 clones. Genomic C. parvum DNA was subjected to partial digestion with Tsp5901 re- striction enzyme (recognition site, AATT; New England BioLabs, Beverly, Mass.) ...
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Cloning, expression and activity of ATP binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti
... ATP-binding protein was successfully cloned. Expression and detection of ABP and Cry11Aa Cry11Aa crystal was expressed and purified from the recombined Bt strain that showed a 72 kDa band ...prokaryotic ...
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Cloning, Expression, and Homology Modeling of GroEL Protein from Leptospira interrogans Serovar Autumnalis Strain N2
... GroEL, expression, homology modeling, leptospirosis, serodiagnosis Introduction Leptospirosis is a global zoonotic bacterial disease caused by Leptospira species, affecting both urban and rural areas with ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... prokaryotic expression protein by induction was detected by mass spectrometry, which had an isoelectric point of ...nifK protein was successfully expressed in a prokaryotic expression ...
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... DISCUSSION Sera from mice infected with H. bilis react with a number of proteins that could serve as antigens for serodiagnosis, if cloned and expressed in recombinant form. Membrane anti- gens are logically favored ...
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Cloning and expression of an equine herpesvirus 1 origin-binding protein.
... The product of EHV-1 gene 53, which will be referred to as the EHV-1 OBP, also bound specifically to a truncated HSV-1 Oris and recog- nized a consensus binding site similar to that reco[r] ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... Early diagnosis of the disease is of great impor- tance in the prevention of the disease, and planning for effective treatments, in high risk people like im- munocompromised patients and pregnant women. One of the main ...
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Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae
... P30 protein have mainly been fo- cused on understanding its role in cytadherence and gliding motility (9, 11, 16, ...fusion protein. The fusion protein was immunogenic, as it elicited a boostable ...
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Cloning and expression of a chitinbinding protein of "Pseudoalteromonas tunicata" CCUG 44952T
... La identificación, clonación, secuenciación y estudio de un gen de la bacteria halófila moderada Pseudoalteromonas tunicata , cepa CCUG 44952T, que codifique una nueva proteína [r] ...
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Gene cloning and recombinant protein expression of lipase from marine bacterium
... June 2014 Chairman : Adam Leow Thean Chor, PhD Faculty : Biotechnology and Biomolecular Sciences Nowadays salt tolerant enzymes have gained attention since they have advantages in food processing industries and ...
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Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.
... DNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins [r] ...
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Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein
... CSUI_005805 protein was cloned into the bacterial expression vector pQE-31 to express the recombinant protein rCSUI_005805 in a size similar to the predicted molecular mass that was present in ...
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