COLI RNA-POLYMERASE
Localization of Escherichia coli RNA polymerase-binding sites on bacteriophage S13 replicative form I DNA by protection of restriction enzyme cleavage sites.
... SPENCER* Received 12 December 1985/Accepted 23 March 1987 Protection of restriction endonuclease cleavage sites by Escherichia coli RNA polymerase bound to the replicative form I of bact[r] ...
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Localization of Escherichia coli RNA polymerase binding sites on bacteriophage S13 and phiX174 DNA: alignment with restriction enzyme maps.
... COLI RNA POLYMERASE BINDING 685 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The in vitro mRNA hybridization studies of tion fragments showed a higher background, pr[r] ...
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“Host Shutoff” Function of Bacteriophage T7: Involvement of T7 Gene 2 and Gene 0.7 in the Inactivation of Escherichia coli RNA Polymerase
... coli RNA polymerase from an amber suppressor host 011' strain infected with the T7 gene 2 amber mutant phage 2-64 exhibited an inhibited level of activity comparable to the enzyme activi[r] ...
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Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
... nous RNA polymerase and sigma factor 70, it was inter- esting to determine the quantity of template required to get maximum protein production and to compare it to the amount of plasmid required in ...
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I protein: bacteriophage T7-coded inhibitor of Escherichia coli RNA polymerase.
... Although, as will be described in the following section, I protein does not prevent the binding of core polymerase with T7 DNA, our holoenzyme preparation used in this experiment was sat[r] ...
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Transcription Unit Mapping in Bacteriophage T7 I. In Vivo Transcription by Escherichia Coli RNA Polymerase
... 'The results demonstrate the existence of' a single large transcription unit spanning the early region, with a promotor located at the left end of' the T7 genome.. Furthermore, there are[r] ...
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Transcription of polyoma DNA by Escherichia coli RNA polymerase: influence of ionic strength on promoter selection.
... Binding and initiation sites at both ionic strengths were identified; at 0.15 M KCl, transcription was initiated from two major sites, located at 0.99 and 0.06 map unit, whereas at 0.45 [r] ...
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Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.
... We compared the in vitro transcriptional properties of RNAPT515 with those of three other purified enzyme preparations: i RNA polymerase from uninfected non-colicinogenic cells; ii RNA p[r] ...
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Modulation of transcription elongation via the main channel of Escherichia coli RNA polymerase
... The RNA/DNA hybrid leads from the catalytic site and is maintained for approximately nine base ...the RNA from the DNA. The RNA is then extruded through the RNA exit channel which is composed ...
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The C-terminal domain of the Escherichia coli RNA polymerase alpha subunit plays a role in the CI-dependent activation of the bacteriophage λ p(M) promoter.
... and RNA bands were visualized and quantified, following background subtraction, using a PhosphorImager ...activator-independent RNA-I promoter, were: 46 nM wild-type RNAP, 34 nM RNAP aK271E, 54 nM RNAP ...
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Transcription of reconstituted simian virus 40 nucleoprotein complexes.
... coli RNA polymerase is useful for investigating the regulatory mechanisms involved in SV40 gene 675 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The regulatory effec[r] ...
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Escherichia coli σ70 senses sequence and conformation of the promoter spacer region
... E. coli RNA polymerase was purchased from Epicenter ...NaCl. RNA polymerase holoen- zyme was reconstituted by incubating core RNA polymer- ase with equimolar amounts of s 70 and ...
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Identification and Characterization of the Escherichia coli-Expressed RNA-Dependent RNA Polymerase of Bamboo Mosaic Virus
... concentration, 0.8 mM) was added to the culture when the cell density reached an optical density at 600 nm of approximately 0.8 to 1 to induce the expression of the lysogenic T7 RNA polymerase that, in ...
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PCR-mediated Expression of the Human GM-CSF Gene in Escherichia coli
... Moreover RNA polymerase of bacterio- phage T7 is very selective for specific promoters that are rarely encountered in DNA unrelated to T7 ...T7 RNA polymerase elongates chains about five times ...
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Mycobacterial RNA Polymerase Requires a U Tract at Intrinsic Terminators and Is Aided by NusG at Suboptimal Terminators
... GC-rich RNA hairpins followed immediately by a 7-to-9-nucleotide (nt) U-rich “U-tract,” play principal roles of punctuating and regulating transcription in most ...their RNA polymerases stop at noncanonical ...
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Genetic analysis of gene 1.2 of bacteriophage T7: isolation of a mutant of Escherichia coli unable to support the growth of T7 gene 1.2 mutants.
... The products of at least six genes of phage T7 are essential for T7 DNA replication: gene 1 RNA polymerase, gene 2 inhibitor of Escherichia coli RNA polymerase, gene 3 endonuclease, gene[r] ...
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Schliebner, Ivo (2008): Identifizierung und Charakterisierung von Chloroplastidären Proteinkinasen mit dem Schwerpunkt auf der PPPK10. Dissertation, LMU München: Fakultät für Biologie
... und deren Regulation (Sigma‐Faktor ZmSig2B aus Mais, Beardslee et al., 2002) beteiligt sind, wurden als dual dirigiert in den Mitochondrien und Chloroplasten gefunden. Umso interessanter war die Beobachtung des dualen ...
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Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... virus polymerase to be found near or at Pol II transcription sites in the ...viral polymerase. Moreover, our influenza virus polymerase-specific antibodies do not distinguish between en- gaged and ...
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Modification of RNA polymerase from Escherichia coli by pre-early gene products of bacteriophage T5.
... Early genes 958 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest RNA polymerase from cells of Escherichia coli infected with T5 were recovered as a complex with two pre-[r] ...
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Purification of Global Regulator, Spx, and RNA Polymerase from Staphylococcus aureus for Use in In Vitro Transcription of Redox Genes
... polymerase cannot be easily overexpressed in recombinant form using the T7 polymerase system in E. coli, as was employed for Spx overexpression. There was also no readily available assay for RNAP ...
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