collagen gel
Evaluation of Human Breast Adenocarcinoma (MCF-7) Cells Proliferation in Co-Culture with Human Adipocytes in Three Dimensional Collagen Gel Matrix: Norepinephrine as a Lipolytic Factor
... Recently, some studies have demonstrated that adipocytes promoted the growth of breast carcinoma cells but preadipocytes had the opposite effect, inhibiting proliferation of breast carcinoma cells [15-17]. The in vivo ...
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A novel collagen gel based measurement technique for quantitation of cell contraction force
... of collagen gels containing stimulated fibroblasts and a microscopy imaging system to estimate the gel ...The collagen matrix used in the model provides a physiological environment for fibroblast ...
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Mechatronic devices for the investigation of fibroblast populated collagen gel contraction and the effects of mechanical loading.
... the fibroblasts contract down the collagen matrix. Forces generated by the contraction of a tethered FPCL whilst floating in culture medium have been measured directly (Vandenberg, 1988,1989,1995, Kasugai et al, ...
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Efficient Transmicrocatheter Delivery of Functional Fibroblasts with a Bioengineered Collagen Gel Platinum Microcoil Complex: Toward the Development of Endovascular Cell Therapy for Cerebral Aneurysms
... Straight MicroPlex platinum microcoils (0.0017-inch core wire; 0.0095-inch helix diameter) (MicroVention, Aliso Viejo, Calif) were placed into modified polyvinyldine diflouride hollow fiber tubes (1-mm diameter, 20 –30 ...
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Effect of budesonide on fibroblast-mediated collagen gel contraction and degradation
... After 5 days of culture, the media in which the col- lagen gels had been cultured were harvested and used to assess MMP release and conversion to active forms. Under control conditions (serum-free culture medium), MMP-1, ...
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Osteopontin is produced by rat cardiac fibroblasts and mediates A(II) induced DNA synthesis and collagen gel contraction
... antibody (20–100 mg/ml kindly provided by Thomas Borg, University of South Carolina) or nonimmune rabbit IgG (20–100 mg/ml) were preincubated for 30 min at 378C with cardiac fibroblasts before their incorporation into ...
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Hormonal control of human colon carcinoma cell growth in serum-free medium (nude mice/primary culture/differentiation/collagen gel)
... This result and the demonstration that a hormone-supplemented, serum-free medium allows better growth of HC84S cells than does serum-containing medium raise the possibility that there ar[r] ...
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An ex vivo continuous passive motion model in a porcine knee for assessing primary stability of cell free collagen gel plugs
... Moreover, little is known about the required motion cycles for testing the mechanical properties of cartilage repair constructs. The means to assess the early post- operative phase after cartilage regenerative procedures ...
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EFFECT OF COLLAGEN I GEL ON APOPTOSIS OF RAT HEPATIC STELLATE CELLS
... I collagen gel and were cultured in Dulbecco medium (DMEM, Sigma, Prague) containing 10% fetal bovine serum, 4 mM glutamine, 100 U/ml penicillin and 100 μg/ml ...
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Millicurrent stimulation of human articular chondrocytes cultivated in a collagen type I gel and of human osteochondral explants
... and collagen type-II gene expression, which is character- istic for hyaline cartilage, stayed elevated, while expres- sion of the collagen type-I gene, indicating dedifferentiated chondrocytes, was slowly ...
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Collagen Mediated Platelet Aggregation EFFECTS OF COLLAGEN MODIFICATION INVOLVING THE PROTEIN AND CARBOHYDRATE MOIETIES
... the collagen-platelet interaction, the effects of collagen modification on platelet aggregation have been ...soluble collagen is effective at about 20 nM in mediating platelet aggregation in human ...
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Nature and specificity of the immune response to collagen in type II collagen induced arthritis in mice
... denatured collagen was indistinguishable from that seen in arthritic mice. Humoral immunity was assessed by an ELISA assay for antibodies to collagen. The immunoglobulin M (IgM) response peaked at 2 wk and ...
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Authentication of collagen VI antibodies
... Additional file 1: Figure S1. Immunostaining transfected cells for anti- body specificity. HEK-293 cells transfected with cDNAs for COL6A1 (panels A-E), COL6A2 (F-J) and COL6A3 (K-O) were stained for α1 chains (A, F and ...
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The different forms of collagen and gelatin
... Swelling of limed calfskin gelatin - ashfree.... XXIII See Tabl,zs XX.X:III- XXXVIII.[r] ...
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Confined compression of collagen hydrogels
... Collagen (type I) was prepared from rat tail tendon and hydrogels (1 ml volume) set in standard 24-well tissue culture plates according to Elsdale and Bard (1972). Samples of diameter 16 mm, radially confined in ...
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Activation of hageman factor by collagen
... of collagen structure from triple helical to random coil form, it is suggested that the native form of collagen is essential for the ability to activate Hageman ...of collagen. In contrast, since ...
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Lymphokine stimulation of collagen accumulation
... enhanced collagen accumulation by human embryonic lung fibroblasts (WI-38), as measured by hydroxyproline content of fibroblast monolayers, [14C] proline incorporation into soluble collagen and collagenase ...
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Collagen epitope expression on B cells is sufficient to confer tolerance to collagen induced arthritis
... the collagen type II (CII) amino acids 259 – 270 peptide cloned into the Ii (LNT-Igk-CII) and the control vector with the original class II-associated invariant chain peptide (CLIP) sequence ...
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Preparation of chitosan gel
... Abstract. Aerogel conditioning of the chitosan makes it possible to prepare porous solids of significant specific surface. The increase in the chitosan concentration or the degree of acetylation decreases the specific ...
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Agarose Gel Electrophoresis
... the gel has already been ...the gel is easier and gets the job done. If you need to stain the gel afterwards, refer to the gel staining ...
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