colony-forming assay
Determination of Infectious Retrovirus Concentration from Colony-Forming Assay with Quantitative Analysis
... The colony formation assay is the most commonly used titration method for defining the concentration of replication-incompetent murine leukemia virus-derived retroviral ...and colony formation titer ...
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Identification of mouse colony-forming endothelial progenitor cells for postnatal neovascularization: a novel insight highlighted by new mouse colony-forming assay
... EPC colony clusters: large-EPC- CFUs and small-EPC-CFUs (Figure 1a, data not ...EPC colony-forming capacity than ...the colony-forming potential could be assessed by the frequency of ...
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Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony forming assay
... up assay systems that can identify and characterize the progenitor cells with multipotent differentiation potential from uninduced metanephric mesenchyme using Wnt4 ...
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Targeting hexokinase 2 enhances response to radio-chemotherapy in glioblastoma
... Loss of HK2 sensitizes tumors to chemotherapy and radiation in vitro and in vivo Using a colony-forming assay to measure clonogenic potential and cell viability, radiation significantly [r] ...
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Interleukin-33 regulates hematopoietic stem cell regeneration after radiation injury
... Background: IL-33 is a pleiotropic cytokine of the IL-1 family, which has been reported to implicate in both innate and adaptive immune responses. Recent studies suggest IL-33 is crucial for regulation of myelopoiesis ...
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Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis
... semi-solid colony-forming assay in Xenopus laevis to clarify the existence of colony-forming unit-erythroid cells, the functional erythroid progenitors identified in vitro ...this ...
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SKA1 promotes malignant phenotype and progression of glioma via multiple signaling pathways
... Methods: Bioinformatic analysis was carried out based on the RNA-seq data and corresponding clinical data from GEO, TCGA and CGGA databases. Western blot was performed to analyze the expression of SKA1 in clinical ...
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Bone marrow derived extracellular vesicles activate osteoclast differentiation in traumatic brain injury induced bone loss
... 1)]. Colony forming assay and osteoclast differentiation assay was performed as mentioned above in presence (20 μg/ml) or absent of ...The colony forming assay was stained ...
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CELL PROLIFERATION STUDIES ON HUMAN BREAST CANCER CELLS (MCF 7 AND MDA MB231) USING CURCUMIN
... (PCNA) assay and colony forming ...PCNA assay, curcumin slows cancer cell proliferation and in colony forming study, colonies were stained with crystal violet and the number of ...
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Effects of the common polymorphism in the human aldehyde dehydrogenase 2 (ALDH2) gene on the lung
... The human surgical samples assessed, included 26 lung and 23 bronchial samples. DNA was isolated from all samples and genotyped for the ALDH2 polymorphism (with ALDH2/ALDH2 alleles i.e. has functional ALDH2, heterozygous ...
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Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units
... bioluminescence assay was demonstrated previously ...CFU assay is used primarily to determine the pres- ence of granulocyte-macrophage (GM) progenitor cells as a predictor of granulocyte neutrophil ...
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A high throughput serum bactericidal assay for antibodies to Haemophilus influenzae type b
... the colony counts obtained with digital camera images were highly corre- lated (r = ...the colony counts determined with the scanner were slightly lower than the true ...
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The effect of acute respiratory distress syndrome on bone marrow-derived mesenchymal stem cells
... accomplish this, the supernatants of samples were spun down to remove any remaining cells and stored at −80 °C until simultaneous analysis. Samples were run on a BioPlex 200 system (Bio-Rad, Hercules, CA; ...
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Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells
... These evidences have suggested that low oxygen ten- sion seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in SDSCs. But, the effect of low oxygen tension on the ...
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BCG directly induces cell cycle arrest in human transitional carcinoma cell lines as a consequence of integrin cross-linking
... BCG attaches to the cell surface via a fibronectin bridge linking mycobacterial receptors to cell surface integrins.[12-14] Mycobacterial binding of multiple integrin bound fibronectin molecules can affect crosslink- age ...
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Galectin 9 prolongs the survival of septic mice by expanding tim 3 expressing natural killer T cells and PDCA 1+ CD11c+macrophages
... CLP appears to be a reliable and clinically relevant animal model of the human septic condition because it produces an endogenous polymicrobial infection similar to clinical peritonitis and sepsis. Thus, sepsis was in- ...
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Nafamostat mesylate, a serine protease inhibitor, demonstrates novel antimicrobial properties and effectiveness in Chlamydia induced arthritis
... Arthritis was induced in the rats by the intra-articular injection of live Chlamydia packaged in Lewis rat syno- vial fibroblasts as previously described [2,3]. Briefly, C. trachomatis serotype L2 was inoculated into ...
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Heavy metal exposure reverses genetic resistance to Chlamydia induced arthritis
... The Dako IDEIA™ PCE Chlamydia Kit (Dako, Ely, Cambridge- shire, UK) was used for determining the clearance of the path- ogen. Seven days after the intra-articular injection of Chlamydia, the HgCl 2 -exposed and ...
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Efficacy of autologous stem cell-based therapy for osteonecrosis of the femoral head in sickle cell disease: a five-year follow-up study
... fibroblast colony-forming units in the stromal compartment of the bone marrow of the iliac crest were decreased in pa- tients who were receiving corticosteroids or who abused alcohol ...
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EFFECT OF CURCUMIN (CURCUMA LONGA) AGAINST HUMAN PULMONARY CARCINOMA CANCER CELLS: AN IN VITRO COLONY FORMING AND APOPTOSIS ASSAY
... It is a potent antioxidant, anti-inflammatory, analgesic and anticancer agent used in traditional medicine. It has been shown to suppress cellular transformation, proliferation, invasion, angiogenesis and metastasis ...
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