Comparison between PCR and virus isolation
Reverse transcription PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation
... A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools.. All three t[r] ...
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Dengue Virus Detection Using Whole Blood for Reverse Transcriptase PCR and Virus Isolation
... MATERIALS AND METHODS Clinical samples. As part of a routine diagnostic service for RT-PCR and serological DENV diagnostics, we identified and obtained 108 blood specimens from pediatric patients with suspected ...
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Comparison of Viral Isolation and Multiplex Real Time Reverse Transcription PCR for Confirmation of Respiratory Syncytial Virus and Influenza Virus Detection by Antigen Immunoassays
... syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A ⴙ B and Binax NOW RSV assays over two successive respiratory ...
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A comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
... both isolation and identification of Candida species, based on the pigmentation of the colonies with different colors, which is due to different enzyme activities in Candida ...in comparison with other ...
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Excretion of bovine herpesvirus 1 in semen is detected much longer by PCR than by virus isolation
... of virus excretion varied with the tests used. On the basis of the virus isolation tests, virus excretion periods of several days or weeks and, subsequently, intermit- tent virus ...
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Comparison of Sensitivities of Virus Isolation, Antigen Detection, and Nucleic Acid Amplification for Detection of Equine Influenza Virus
... challenge. Virus isolation was performed in embryonated eggs, and the EID 50 was determined for all positive ...and virus isolation in embryonated eggs proved to be the most sensitive methods ...
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Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)
... and virus isolation methods were used as references for evaluation of the OIA ...A virus in concentrated cells from nasopharyngeal aspirates, the OIA test had a significantly lower sensitivity than ...
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Effectiveness of Reverse Transcription PCR, Virus Isolation, and Enzyme Linked Immunosorbent Assay for Diagnosis of Influenza A Virus Infection in Different Age Groups
... of virus isolation in cell culture and its additional importance for identification of the predominant circulating types, subtypes, and strains of influenza virus and for formulation of vaccine for ...
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Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
... signs, virus isolation and electron microscopy are commonly used along with serological ...example, virus isolation can be unsuccessful at times, even when virus-like particles are ...
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Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA
... Epstein-Barr virus (EBV)-associated ...real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification ...nested PCR samples were used as negative ...differences ...
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Comparison between Two Amplification Sets for Molecular Diagnosis of Toxoplasmosis by Real Time PCR
... 2004. Comparison of two widely used PCR primer systems for detection of Toxoplasma in amniotic fluid, blood, and ...Real-time PCR for diagnosis and follow-up of Toxoplasma re- activation after ...
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Interlaboratory Comparison of Six Real Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA
... Accepted manuscript posted online 18 April 2018 Citation Jaworski JP, Pluta A, Rola-Łuszczak M, McGowan SL, Finnegan C, Heenemann K, Carignano HA, Alvarez I, Murakami K, Willems L, Vahlenkamp TW, Trono KG, Choudhury B, ...
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Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus
... a comparison using only one assay design targeting only one of a number of viruses for which such a method may have clinical ...real-time PCR, seen can be accounted for entirely by the small total sample ...
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Comparison of Different Methods of Isolation of DNA of Commonly Encountered Candida Species and Its Quantitation by Using a Real Time PCR Based Assay
... a comparison of the molecular diagnoses obtained by a real-time PCR-based method to the results of blood culture, the sensitivity and specificity of the molecular method with a ...in comparison to ...
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Comparison of Immunohistochemistry and Virus Isolation for Diagnosis of West Nile Virus
... live virus is not present in formalin- fixed ...that virus isolation still works on severely autolyzed tissue while immunohistochemistry results may be ...
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Comparison of PCR, Immunofluorescence Assay, and Pathogen Isolation for Diagnosis of Q Fever in Humans with Spontaneous Abortions
... real-time PCR, and IFA turned out to be better tests than pathogen isolation for the rapid and reliable diagnosis of Q fever in women with sponta- neous ...Real-time PCR, with specificity equal to ...
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Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens
... TaqMan PCR and that TaqMan PCR may be inhibited by whole-blood DNA ...TaqMan PCR by whole-blood DNA was also reported by sev- eral other groups (8, 9, ...LightCycler PCR as an alternative ...
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Comparison of Herpes Simplex Virus PCR with Culture for Virus Detection in Multisource Surface Swab Specimens from Neonates
... plex virus (HSV) culture or PCR for testing of swabs of the conjunctivae, mouth, na- sopharynx, and rectum (surface swabs) from ...HSV PCR with those of HSV culture with surface swabs from ...by ...
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Economic comparison of enzyme immunoassay and virus isolation procedures for surveillance of arboviruses in mosquito populations
... However, the use of two different primary isolation cell culture assays per mosquito pool increased the estimated total cost for virus isolation and identification to.. $4.48 to $9.48 pe[r] ...
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Development and comparison of a real-time PCR assay for detection of Dichelobacter nodosus with culturing and conventional PCR: harmonisation between three laboratories
... diagnosis. PCR-based detection using conventional PCR has been used at our institutes, but the method was laborious and there was a need for a faster, easier-to-interpret ...real-time PCR assay for ...
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