conformational epitopes
Neutralizing human monoclonal antibodies to conformational epitopes of human T-cell lymphotropic virus type 1 and 2 gp46.
... recognized conformational epitopes (4, ...to epitopes present only on correctly folded Env proteins expressed on the cell ...to conformational epitopes is suggested by experiments ...
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Human Monoclonal Antibodies That Inhibit Binding of Hepatitis C Virus E2 Protein to CD81 and Recognize Conserved Conformational Epitopes
... This study provides support for the contention that the ma- jority of antibodies capable of inhibiting the interaction of HCV E2 with CD81 are developed to conformational epitopes conserved across genotypes ...
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Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis
... to conformational epitopes that was otherwise masked in the entire cohort predominantly consisting of low-titer anti-MOG reactive antibodies (table 1 Figure ...immunodominant epitopes became ...
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Identification of Immunodominant and Conformational Epitopes in the Capsid Protein of Hepatitis E Virus by Using Monoclonal Antibodies
... 4B2 than is the GST-ORF2.1 protein (Fig. 2) and might thus be expected to induce strong responses to the conformational ORF2.1 epitope, whereas C2 (aa 225 to 660) is completely unreactive with these MAbs, at least ...
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Monoclonal anti-idiotypes induce neutralizing antibodies to enterovirus 70 conformational epitopes.
... As illustrated in Table 4, the immunization of syngeneic mice with MAb2s elicited a neutralizing immune response specific for EV-70.. Mice which received MAb2-2 produced the best neutral[r] ...
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Membrane bound modified form of clade B Env, JRCSF is suitable for immunogen design as it is efficiently cleaved and displays all the broadly neutralizing epitopes including V2 and C2 domain dependent conformational epitopes
... on the viral glycoprotein Env be developed from a wide variety of HIV-1 sub-types. However, currently avail- able candidate immunogens have limited neutralization breadth and potency necessitating the search for newer ...
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Folding, assembly, and intracellular trafficking of the human immunodeficiency virus type 1 envelope glycoprotein analyzed with monoclonal antibodies recognizing maturational intermediates.
... or conformational epitopes on monomeric or oligomeric human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins were screened for their recognition of maturational ...relative conformational ...
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Improved reactivity of hepatitis C virus core protein epitopes in a conformational antigen-presenting system.
... several epitopes in the N-terminal portion of the nucleocapsid protein which are predominantly recognized by sera of patients infected with hepatitis C virus ...low conformational requirements for the ...
5
Simian Immunodeficiency Virus (SIV) Envelope-Specific Fabs with High-Level Homologous Neutralizing Activity: Recovery from a Long-Term-Nonprogressor SIV-Infected Macaque
... immunodominant epitopes of the SIV and HIV envelope gly- coproteins may ...SIV. Conformational epitopes may be the target of more broadly neutralizing antibody ...to conformational ...
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The impact of structural integrity and route of administration on the antibody specificity against three cow’s milk allergens - a study in Brown Norway rats
... consequence conformational epitopes will be lost while linear epitopes are still ...versus conformational epitopes of the three cow’s milk allergens by means of ...ational ...
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Identification of B-Cell Epitopes on Virus-Like Particles of Cutaneous Alpha-Human Papillomaviruses
... linear epitopes may also be explained by potentially improperly folded immunogen ...against conformational epitopes on VLPs of HPV types 6, 11, 16, and 18 (12–16, 32), so a major impact by the ...
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5597.pdf
... dominant conformational epitopes in EC1 and EC2 respectively. Both epitopes were flanked by calcium-binding sites in the model and were in opposite faces of the ...EC1 epitopes from the dimer ...
103
Envelope Proteins Containing Single Amino Acid Substitutions Support a Structural Model of the Receptor-Binding Domain of Bovine Leukemia Virus Surface Protein
... In general, recognition by all the conformation-specific an- tibodies correlated with syncytium-inducing ability of an Env variant. Variants that induced few syncytia and that were not proteolytically processed were ...
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Monoclonal Antibodies against the Adeno-Associated Virus Type 2 (AAV-2) Capsid: Epitope Mapping and Identification of Capsid Domains Involved in AAV-2–Cell Interaction and Neutralization of AAV-2 Infection
... linear epitopes of A1, A69, and B1 which reside in VP1, VP2, and VP3, respectively, using gene fragment phage display library, peptide scan, and peptide competition ...against conformational epitopes ...
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Localization of rotavirus VP4 neutralization epitopes involved in antibody-induced conformational changes of virus structure.
... We examined the direct effect of NPlb MAb binding on virus to structure and characterized four neutralization escape mutants selected with the neutralization MAbs 9F6, 7G6, 10G6, and 23.[r] ...
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Conformational Occlusion of Blockade Antibody Epitopes, a Novel Mechanism of GII.4 Human Norovirus Immune Evasion
... Occluded epitopes of other human RNA viruses are essential for receptor binding, viral fusion, and capsid assembly/disassembly, functions essential for infection/replica- tion ...prevent conformational ...
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A Conformational Switch in Human Immunodeficiency Virus gp41 Revealed by the Structures of Overlapping Epitopes Recognized by Neutralizing Antibodies
... The membrane-proximal external region (MPER) of the human immunodeficiency virus (HIV) envelope glyco- protein (gp41) is critical for viral fusion and infectivity and is the target of three of the five known broadly ...
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Neutralizing Antibody Escape during HIV-1 Mother-to-Child Transmission Involves Conformational Masking of Distal Epitopes in Envelope
... For F535, we found that V1 to V3 of B res conferred escape from maternal plasma. To test whether V1, V2, and V3 were linear targets of maternal NAbs, we performed competition neutraliza- tion assays using peptides ...
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SPECTROSCOPIC AND ELECTROCHEMICAL INVESTIGATION OF BINDING OF NEVIRAPINE WITH BOVINE SERUM ALBUMIN
... The primary structure of BSA contains a single chain of about 582 amino acid residues. [30] The secondary structure of BSA is formed by 67% of α-helix of six turns and 17 disulphide bridges. [31] When ligand binds to ...
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Gold and Magnetite Nanoparticles as Sensors of Conformational Changes in Proteins
... However, the attaching of proteins to the surface of metallic nanoparticles is currently a real challenge. Proteins are either hydrophobic or hydrophilic, they have either positive or negative charge and their structures ...
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