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Correlative light-electron microscopy

FerriTag is a new genetically encoded inducible tag for correlative light electron microscopy

FerriTag is a new genetically encoded inducible tag for correlative light electron microscopy

... by light and electron ...for correlative light-electron ...recombinant electron- dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced ...

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Development of a new genetically encoded tag for correlative light electron microscopy

Development of a new genetically encoded tag for correlative light electron microscopy

... as correlative samples were processed in a way where only the cell-of-interest is tracked throughout the entire workflow and no untransfected cells are seen in-resin to ...on electron microscopy ...

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Fabrication and quantitative correlative light-electron microscopy of novel plasmonic nanoparticles

Fabrication and quantitative correlative light-electron microscopy of novel plasmonic nanoparticles

... flexible correlative imaging technique to study various MNP in different dielectric environments at single NP level; ii) fabricating AgNP with different shapes and sizes as well as AgNP dimers with various ...

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Localization of active endogenous and exogenous beta-glucocerebrosidase by correlative light-electron microscopy in human fibroblasts

Localization of active endogenous and exogenous beta-glucocerebrosidase by correlative light-electron microscopy in human fibroblasts

... this correlative microscopy approach resulted in the localization of the enzyme to specific subcellular compartments, without the need for additional ...

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Ultrastructural Imaging of Salmonella-Host Interactions Using Super-resolution Correlative Light-Electron Microscopy of Bioorthogonal Pathogens

Ultrastructural Imaging of Salmonella-Host Interactions Using Super-resolution Correlative Light-Electron Microscopy of Bioorthogonal Pathogens

... the electron micrograph is of the order of 1 nm, that of fluorescence microscopy is limited by the Abbe diffrac- tion limit of half the photon wavelength (l & 250 nm), [14] result- ing in a resolution gap ...

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Correlative single‐molecule localization microscopy and electron tomography reveals endosome nanoscale domains

Correlative single‐molecule localization microscopy and electron tomography reveals endosome nanoscale domains

... limited light microscopy. Single molecule localization microscopy (SMLM) offers nanoscale resolution but data interpretation is often inconclusive when the ultra- structural context is ...missing. ...

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Correlative Light- and Electron Microscopy with chemical tags

Correlative Light- and Electron Microscopy with chemical tags

... 3. Discussion The functional assignment of ultrastructural data with fluores- cence microscopy has the capacity to transform our interpretations of biological structure and function. However, the functional ...

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A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario

A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario

... Discussion Merits of the workflow The correlative examination of a small biological speci- men offers a number of advantages in the analysis, visualization, and validation of results. The option of topographical ...

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Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy

Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy

... Fluorescence microscopy reveals molecular expression at nanometer resolution but lacks ul- trastructural context ...results. Electron microscopy provides this contextual subcellular detail, but ...

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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

... SR microscopy and EM 23–26 ...incoming light and converts the radiation to heat, thereby raising the temperature of the vitrified sample above the devitrification temperature ...

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Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy

Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy

... The nanoparticles were found in endocytic compartments (Fig. 6b ), and could be resolved at an individual particle level. The unique structure of the particles proved helpful in identification, meaning that any ...

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Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells

Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells

... After a brief rinse in 1% sodium sulphate in 0.05 M PB (w/v), the coverslips were washed twice in distilled water, dehydrated through an ascending series of ethanol and propylene oxide prior to in fi ltration with epoxy ...

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Investigating CNS synaptogenesis at single synapse resolution by combining reverse genetics with correlative light and electron microscopy

Investigating CNS synaptogenesis at single synapse resolution by combining reverse genetics with correlative light and electron microscopy

... The light microscopy analysis of Brp short ::GFP localization at central ms neuron synapses reveals distinct labeling of individual puncta in the size range of single active ...

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Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy

Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy

... lating fluorescent viral antigens with electron-dense structures. Localization of fluores- cent protein-tagged HIV Gag identified bona fide donor cell-target cell conjugates and characterized the various structural ...

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Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy.

Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy.

... cryo-correlative light and electron microscopy, we distinguished intact excitatory and inhibitory synapses of cultured hippocampal neurons, and visualized the in situ 3D organization of ...

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Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo

Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo

... The workflow also delivers advances in correlative microscopy. In post-embedding CLEM experiments on thin sections, it is neces- sary to treat the section for each imaging modality. In practice, this ...

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Correlative Imaging of the Murine Hind Limb Vasculature and Muscle Tissue by MicroCT and Light Microscopy

Correlative Imaging of the Murine Hind Limb Vasculature and Muscle Tissue by MicroCT and Light Microscopy

... The murine hind limb is one of the most widely used preclinical models to study therapeutic angiogenesis. This is mainly due to the anatomical similarities with the human limb and its relatively simple microvascular ...

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The innate immune response against mycobacterial infection : analysis by a combination of light and electron microscopy

The innate immune response against mycobacterial infection : analysis by a combination of light and electron microscopy

... Conclusion In this thesis the zebrafish tail fin infection model is presented, which enables the study of a complex immune response towards (myco)bacterial infection using a combination of light and ...

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A light and electron microscopy study of the yak placentome

A light and electron microscopy study of the yak placentome

... trophoblast epithelial cells were usually columnar or irregular. The cytoplasmic membrane had a relatively smooth surface and was attached closely to the ad- jacent uninuclear trophoblast epithelial cells through tight ...

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Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling

Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling

... scanning electron microscopy, immunocytochemistry, three-dimensional reconstruction, confocal laser-scanning microscope, neural circuit, synapse, neostriatum INTRODUCTION One of the most essential features ...

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