Correlative light electron microscopy (CLEM)
FerriTag is a new genetically encoded inducible tag for correlative light electron microscopy
... by light and electron ...for correlative light-electron ...recombinant electron- dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced ...
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Development of a new genetically encoded tag for correlative light electron microscopy
... as correlative samples were processed in a way where only the cell-of-interest is tracked throughout the entire workflow and no untransfected cells are seen in-resin to ...on electron microscopy ...
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Localization of active endogenous and exogenous beta-glucocerebrosidase by correlative light-electron microscopy in human fibroblasts
... by light micros- copy. 13 As fluorescence microscopy does not provide information about the underlying ultrastructure, in this study a correlative light and electron microscopy ...
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Ultrastructural Imaging of Salmonella-Host Interactions Using Super-resolution Correlative Light-Electron Microscopy of Bioorthogonal Pathogens
... the electron micrograph is of the order of 1 nm, that of fluorescence microscopy is limited by the Abbe diffrac- tion limit of half the photon wavelength (l & 250 nm), [14] result- ing in a resolution gap ...
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Fabrication and quantitative correlative light-electron microscopy of novel plasmonic nanoparticles
... flexible correlative imaging technique to study various MNP in different dielectric environments at single NP level; ii) fabricating AgNP with different shapes and sizes as well as AgNP dimers with various ...
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Correlative single‐molecule localization microscopy and electron tomography reveals endosome nanoscale domains
... limited light microscopy. Single molecule localization microscopy (SMLM) offers nanoscale resolution but data interpretation is often inconclusive when the ultra- structural context is ...missing. ...
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Correlative Light- and Electron Microscopy with chemical tags
... z-localization, correlative array tomography could be employed in which, a series of ultrathin sections (40–100 nm) is examined by CLEM (Wacker and Schroeder, ...
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Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy
... for correlative confocal fluores- cence and 3D electron ...and electron imaging can prove challenging due to the heterogeneous content of the cell and limited resolution of fluorescence ...3D ...
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Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells
... separate light and electron microscopes, resulting in improved localisation accuracy when compared to pre-embedding CLEM performed on whole ...integrated light and scanning electron ...
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Investigating CNS synaptogenesis at single synapse resolution by combining reverse genetics with correlative light and electron microscopy
... for correlative light and 3D electron microscopy (3D ...scanning electron microscopy analysis of ms axons revealed T-bar-shaped dense bodies and other characteristic ...
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Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy
... lating fluorescent viral antigens with electron-dense structures. Localization of fluores- cent protein-tagged HIV Gag identified bona fide donor cell-target cell conjugates and characterized the various structural ...
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Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy.
... a light micro- scope ...the light mi- ...between light and electron microscopes without repeated sample transfer, thus minimizing ice contamination and grid ...
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Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo
... The workflow also delivers advances in correlative microscopy. In post-embedding CLEM experiments on thin sections, it is neces- sary to treat the section for each imaging modality. In practice, this ...
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A correlative approach for combining microCT, light and transmission electron microscopy in a single 3D scenario
... Discussion Merits of the workflow The correlative examination of a small biological speci- men offers a number of advantages in the analysis, visualization, and validation of results. The option of topographical ...
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Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy
... Fluorescence microscopy reveals molecular expression at nanometer resolution but lacks ul- trastructural context ...results. Electron microscopy provides this contextual subcellular detail, but ...
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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins
... SR microscopy and EM 23–26 ...incoming light and converts the radiation to heat, thereby raising the temperature of the vitrified sample above the devitrification temperature ...
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Correlative Imaging of the Murine Hind Limb Vasculature and Muscle Tissue by MicroCT and Light Microscopy
... The murine hind limb is one of the most widely used preclinical models to study therapeutic angiogenesis. This is mainly due to the anatomical similarities with the human limb and its relatively simple microvascular ...
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The innate immune response against mycobacterial infection : analysis by a combination of light and electron microscopy
... Conclusion In this thesis the zebrafish tail fin infection model is presented, which enables the study of a complex immune response towards (myco)bacterial infection using a combination of light and ...
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A light and electron microscopy study of the yak placentome
... trophoblast epithelial cells were usually columnar or irregular. The cytoplasmic membrane had a relatively smooth surface and was attached closely to the ad- jacent uninuclear trophoblast epithelial cells through tight ...
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Correlative analysis of immunoreactivity in confocal laser-scanning microscopy and scanning electron microscopy with focused ion beam milling
... scanning electron microscopy, immunocytochemistry, three-dimensional reconstruction, confocal laser-scanning microscope, neural circuit, synapse, neostriatum INTRODUCTION One of the most essential features ...
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