covalently closed circular
Hepatitis B Virus Covalently Closed Circular DNA Formation in Immortalized Mouse Hepatocytes Associated with Nucleocapsid Destabilization
... relaxed circular; DSL, double stranded linear; CCC, covalently closed circular; CCC-L, covalently closed circular linearized by EcoRI digestion; SS, single stranded; M, ...
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PCR mediated recombination impacts the analysis of hepatitis B Virus covalently closed circular DNA
... Incoming partly double stranded hepatitis B virus (HBV) DNA genomes are completed by the viral polymerase generating relaxed circular DNA (rcDNA). Following translocation to the nucleus, the gaps are repaired by ...
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Covalently Closed Circular DNA Is the Predominant Form of Duck Hepatitis B Virus DNA That Persists following Transient Infection
... Residual hepatitis B virus (HBV) DNA can be detected in serum and liver after apparent recovery from transient infection. However, it is not known if this residual HBV DNA represents ongoing viral replication and antigen ...
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n-Butyrate, a cell cycle blocker, inhibits early amplification of duck hepatitis B virus covalently closed circular DNA after in vitro infection of duck hepatocytes.
... 5 n-Butyrate, a Cell Cycle Blocker, Inhibits Early Amplification of Duck Hepatitis B Virus Covalently Closed Circular DNA after In Vitro Infection of Duck Hepatocytes FABRICE TURIN,1 CHR[r] ...
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Characterization of Nucleosome Positioning in Hepadnaviral Covalently Closed Circular DNA Minichromosomes
... Hepadnaviral covalently closed circular DNA (cccDNA) exists as an episomal minichromosome in the nucleus of virus-infected hepatocytes, and serves as the transcriptional template for the synthesis of ...
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Half-Life of the Duck Hepatitis B Virus Covalently Closed Circular DNA Pool In Vivo following Inhibition of Viral Replication
... small, circular, partially double- stranded DNA ...is covalently linked to protein at its 5⬘ end and contains a nick at its 3⬘ ...a covalently closed circular DNA (cccDNA) form within ...
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Detection, purification, and characterization of two species of covalently closed circular proviral DNA molecules of bovine leukemia virus.
... 3 0022-538X/83/031172-05$02.00/0 Copyright C 1983, American Society for Microbiology Detection, Purification, and Characterization of Two Species of Covalently Closed Circular Proviral D[r] ...
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Recombinant Covalently Closed Circular Hepatitis B Virus DNA Induces Prolonged Viral Persistence in Immunocompetent Mice
... It remains crucial to develop a laboratory model for studying hepatitis B virus (HBV) chronic infection. We hereby produced a recombinant covalently closed circular DNA (rcccDNA) in view of the key ...
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Serum Hepatitis B Virus DNA, RNA, and HBsAg: Which Correlated Better with Intrahepatic Covalently Closed Circular DNA before and after Nucleos(t)ide Analogue Treatment?
... reported that viral rebound occurred in all (21/21) patients with positive serum HBV RNA results at the end of NUC treatment, while only 25% (3/12) of patients with negative serum HBV RNA results at the end of NUC ...
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Identification of an Intermediate in Hepatitis B Virus Covalently Closed Circular (CCC) DNA Formation and Sensitive and Selective CCC DNA Detection
... (HBV) covalently closed circular (CCC) DNA functions as the only viral template capable of coding for all the viral RNA species and is thus es- sential to initiate and sustain viral ...relaxed ...
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Characterization of the Intracellular Deproteinized Relaxed Circular DNA of Hepatitis B Virus: an Intermediate of Covalently Closed Circular DNA Formation
... Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is formed by conversion of capsid- associated relaxed circular DNA (rcDNA) via unknown mechanisms and exists in the nucleus ...
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Nuclear Covalently Closed Circular Viral Genomic DNA in the Liver of Hepatocyte Nuclear Factor 1α-Null Hepatitis B Virus Transgenic Mice
... relaxed circular replication intermediates; L, linear HBV DNA; CCC, covalently closed circular HBV DNA; HNF1 genotype ⫹/⫹, HNF1␣ wild-type mouse; HNF1 genotype ⫹/⫺, HNF1␣ heterozygous mouse; ...
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Monomer and multimer covalently closed circular forms of Rous sarcoma virus DNA.
... 148 du Centre National de la Recherche Scientifique, Institute of Cancerology and Immunogenetics, Villejuif, France Covalently closed circular molecules of viral DNA synthesized in virus[r] ...
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Evaluation of Transcriptional Efficiency of Hepatitis B Virus Covalently Closed Circular DNA by Reverse Transcription-PCR Combined with the Restriction Enzyme Digestion Method
... Virus persistence in chronic hepatitis B patients is due to the sustaining level of covalently closed circular DNA (cccDNA) within the nuclei of infected hepatocytes. In this study, we used a ...
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Alteration of Mature Nucleocapsid and Enhancement of Covalently Closed Circular DNA Formation by Hepatitis B Virus Core Mutants Defective in Complete-Virion Formation
... relaxed circular (RC) DNA genome enclosed within an icosahedral capsid that is, in turn, formed by multiple copies (240 or 180) of the viral capsid or core protein (2, ...a covalently closed ...
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Age-Related Differences in Amplification of Covalently Closed Circular DNA at Early Times after Duck Hepatitis B Virus Infection of Ducks
... rapid covalently closed circular DNA amplification, leading to lower viremias and a slower spread of infection in the liver, and that the slower spread of infection in 3W ducks makes the infection ...
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Covalently closed circular viral DNA formed from two types of linear DNA in woodchuck hepatitis virus-infected liver.
... from circular, partially single-stranded viral DNA by electrophoretic mobility shift assay, since both forms would migrate in a common heteroge- neous “smear,” between fully single-stranded and fully double- ...
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Covalently closed circular DNAs of murine type C retrovirus: depressed formation in cells treated with cycloheximide early after infection.
... A total of 2 x 106 cells per 181 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Formation of viral closed circular supercoiled DNA duplexes and production of progeny v[r] ...
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Roles of the Envelope Proteins in the Amplification of Covalently Closed Circular DNA and Completion of Synthesis of the Plus-Strand DNA in Hepatitis B Virus
... FIG. 1. Effect of ablation of expression of the envelope proteins on the level of fl-RC DNA and cccDNA. Huh7-H1 cells were transfected with plasmids that express either WT or L ⫺ M ⫺ S ⫺ mutant HBV. (A) Immunoblotting ...
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Virus-specific DNA in the cytoplasm of avian sarcoma virus-infected cells is a precursor to covalently closed circular viral DNA in the nucleus.
... VARMUS Department ofMicrobiology, University of California, San Francisco, California 94143 Three principal forms of viral DNA have been identified in cells infected with avian sarcoma v[r] ...
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