CRISPR-Based
Characterization and CRISPR-based genotyping of clinical trh-positive Vibrio parahaemolyticus
... Background: Vibrio parahaemolyticus is a causative agent of gastroenteritis. Most of the clinical isolates carry either tdh and/or trh genes which are considered as the major virulence genes of this pathogen. In this ...
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A set of novel CRISPR-based integrative vectors for Saccharomyces cerevisiae
... of CRISPR-Cas9 plasmids and corresponding repair plasmids that make integration more practical, flexible and ...The CRISPR-Cas9 plasmid is used to introduce a single DSB in a gene encoding an essential ...
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Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans
... ABSTRACT Clustered regularly interspaced short palindromic repeat (CRISPR) meth- odology is not only an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and protein ...
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CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
... The use of the clustered regularly interspaced short palin- dromic repeats (CRISPR)-Cas9 system for genome engineering (Hsu et al. 2014) has greatly facilitated the study of gene func- tion in Caenorhabditis ...
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CRISPR-Based Assays for Rapid Detection of SARS-CoV-2
... Gootenberg et al. utilized the Cas13a instead of Cas9 considering its ability to bind and cleave RNA rather than DNA substrate [26]. This potential leverage this method to be utilized for viral RNA detection directly. ...
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Anti-CRISPR-based biosensors in the yeast S. cerevisiae
... We evaluated the functions of these three anti-CRISPR proteins in the yeast S. cerevisiae. Each of them acted on a yeast codon-optimized version of dSpCas9 (fused to a nuclear localization sequence–NLS) [10] ...
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A Review of CRISPR-Based Genome Editing: Survival, Evolution and Challenges
... 2018). CRISPR/Cas9 has been preferred for a variety of novel functions, including expression of gene activation or repression (La Russa and Qi, ...example, CRISPR/Cas9 Synergistic Activation Mediator (SAM) ...
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Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
... developed CRISPR-Cas9 constructs to disrupt the carot- enoid biosynthesis gene, ...the CRISPR-Cas9 expression ...efficient CRISPR-based genome editing in ...
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Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species
... (CRISPR)- based gene editing tools has revolutionized studies in many Candida species, including ...a CRISPR-Cas9 system on a replicating plasmid that can be used for markerless gene editing in ...
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CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
... developed CRISPR-based Somatic Oncogene kNock In for Cancer Modeling (CRISPR-SONIC), a method for rapid in vivo cancer ...the CRISPR-SONIC system to somatic hepatocytes and found targeted ...
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Keeping CRISPR/Cas on-Target
... mostly based on characterizations of the nucleases used to binding site or DNA cutting assay in vitro or in ...is based on in vitro cleavage of a library followed by deep ...
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CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum
... Thus, in the case of var2csa, the intron is not required for transcriptional silencing or activation. Our data do not support earlier studies that used episomes and transgenes to demonstrate the requirement of the var ...
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Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes
... Different RNP concentrations were tested in the transfor- mation reaction ranging from 45 to 1520 nM. As a result, 110 nM RNP concentration provided the highest CFU while higher RNP concentrations gradually decreased the ...
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Delivery systems of CRISPR/Cas9-based cancer gene therapy
... of CRISPR machin- ery delivered via lentiviruses: they described an innova- tive approach to model invasive lobular breast carcinoma by intraductal injection of lentiviral vectors encoding Cre recombinase, the ...
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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
... The ease of generating CRISPRi-based knockdowns in Caulobacter crescentus should now enable a variety of whole-genome studies including systematic knockdowns of every gene, as has been done for B. subtilis (15). ...
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Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
... Replicable plasmid endowed by S. cerevisiae ARS. Based on a recent study on I. orientalis population genomics, there was a high similarity of the genome sequences between I. orientalis and S. cerevisiae (13). ...
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Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos
... Although CRISPR/Cas9-based germline editing is currently far from being efficient or safe enough to warrant clinical applications, we believe that the same technology could be extremely useful for basic ...
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On the Origin of Reverse Transcriptase Using CRISPR Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires
... the CRISPR spacers is a general, unresolved ...most CRISPR arrays (over 90% on average, according to the latest comprehensive survey [43]) have no detectable matches in known ...of CRISPR-Cas ...
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How to Clear HBV cccDNA: CRISPR/Cas9 Might Be Promising
... of CRISPR/Cas9 to treat ...the CRISPR/Cas9 system facilitates clearance of intrahepatic HBV templates in vivo, pAAV/HBV ...by CRISPR/Cas9 nuclease efficiently inhibits viral replication, precccDNA ...
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On the global CRISPR array behavior in class I systems
... dataset. CRISPR-Cas systems are not ubi- quitous in nature, and occur in approximately 46% of se- quenced microbial genomes, and 90% of available archaeal ...of CRISPR-Cas systems, which explains the ...
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