Crispr-cas System
A New Group of Phage Anti CRISPR Genes Inhibits the Type I E CRISPR Cas System of Pseudomonas aeruginosa
... I-E CRISPR-Cas system of strain SMC4386 was isolated, we were able to test di- rectly the type I-E anti-CRISPR activity of individual genes from phage anti-CRISPR regions by expressing ...
7
Advances in Engineering the Fly Genome with the CRISPR-Cas System
... the CRISPR- Cas system now provides the ability to directly edit endoge- nous genomic sequences and precisely incorporate exogenous DNA, which will undoubtedly lead to a new generation of ...
18
<p>How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance</p>
... the CRISPR-Cas system and antibiotic resistance in some species such as enterococci, 72,74 but, in other studies, there was no signi fi cant relation, such as in ...three CRISPR loci including ...
11
Functional Characterization of CRISPR Cas System in the Ethanologenic Bacterium Zymomonas mobilis ZM4
... the CRISPR-Cas system in ...the CRISPR array P1 in placement of the original spacer 1 and/or ...modified CRISPR array to express and to generate the special crRNA in the bacterial ...
12
Cloning free CRISPR/Cas system facilitates functional cassette knock in in mice
... The CRISPR/Cas system was initially reported as an adaptive immune system in bacteria, consisting of three components including Cas9 nuclease and two small RNAs, CRISPR RNA (crRNA), ...
11
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system
... Target specificity is an important issue for all genome editing technologies, including CRISPR/Cas. Recently, a number of reports have systematically examined specifi- city of the CRISPR/Cas ...
10
Escherichia Coli CRISPR/CAS System Evolved with Bacteriophage: A Case Report
... In this case study, we demonstrated the results of sequence analysis which can help understanding the presence of CRISPR-me- diated anti-phage ability in pathogenic and non-pathogenic strains of E. coli . We ...
7
An Attenuated CRISPR Cas System in Enterococcus faecalis Permits DNA Acquisition
... lack cas genes associated with clustered regularly interspaced short palin- dromic repeats (CRISPR) and CRISPR-associated protein (CRISPR-Cas) systems, which act as adaptive immune ...
19
Function of the CRISPR Cas System of the Human Pathogen Clostridium difficile
... I CRISPR-Cas systems are the most diverse and have been classified into ...I-B CRISPR- Cas system is found in clostridia as well as in methanogenic and halophilic archaea and is defined ...
16
Cas6 specificity and CRISPR RNA loading in a complex CRISPR Cas system
... immune system, providing protection against viruses and other mobile genetic ...III CRISPR-Cas systems, CRISPR RNA (crRNA) is generated by cleavage of a primary transcript by the Cas6 ...
10
Mechanism of spacer integration links the CRISPR/Cas system to transposition as a form of mobile DNA
... Similarly, CRISPR spacer acquisition in vivo results in duplication of the CRISPR repeat in the target, again suggesting double-ended integration (where the ends correspond to the two ends of a single ...
5
Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus
... replaced with alanine. We also constructed the H19A mutant, since it was shown that the corresponding mutation (R21A) in M. kandleri Csm3 abolished binding of single-stranded RNA (Hrle et al., 2013). We expressed each ...
67
CRISPR-Cas defense system and potential prophages in cyanobacteria associated with the coral black band disease
... a CRISPR-Cas system of Escherichia coli can prevent prophage induction without killing the host bacterium when both processes, virus replication and CRISPR-Cas defense, are activated at ...
14
CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery
... and CRISPR-associated (Cas) systems are of great interest to the biotechnology community because of their use in genome ...the CRISPR/Cas system originates, it functions as an adaptive ...
104
Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins
... karyotic CRISPR-Cas systems have evolved to select short sequences (protospacers) of the invading DNA and inte- grate them as spacer sequences into CRISPR loci to provide sequence-specific immunity ...
11
Phylogenetic Distribution of CRISPR Cas Systems in Antibiotic Resistant Pseudomonas aeruginosa
... MDRPA CRISPR-Cas-deficient strains har- bored pOZ176-like plasmids, these plasmids did not contain antibiotic resistance ...aeruginosa CRISPR-Cas systems have historically been FIG 5 ...
13
CRISPR transcript processing: a mechanism for generating a large number of small interfering RNAs
... coli CRISPR spacers indeed do not match sequences of known ...in CRISPR array by means of genetic engineering, E. coli CRISPR/ Cas system becomes ...that CRISPR/Cas ...
11
Keeping CRISPR/Cas on-Target
... of CRISPR/Cas system (Cong et ...based CRISPR interference (CRISPRi) is proven to be more significant than classic RNAi-based ...2013). CRISPR/ Cas system has also been ...
13
Programmable Removal of Bacterial Strains by Use of Genome Targeting CRISPR Cas Systems
... I-F CRISPR-Cas system ...III-A system, infecting cells with viral particles encoding a genome-targeting spacer slowed the growth of the culture under selecting conditions and led to ...
9
CRISPR Cpf1 proteins: structure, function and implications for genome editing
... Inducing precise single-base changes or base substitu- tions remains a challenging task. This is due to the inef- ficiency of HDR in eukaryotic cells [123]. Also, HDR requires the construction of a 500–1000 bp repair ...
21