CRISPR-RNA
Cas6 specificity and CRISPR RNA loading in a complex CRISPR Cas system
... III CRISPR-Cas systems, CRISPR RNA (crRNA) is generated by cleavage of a primary transcript by the Cas6 endonuclease and loaded into multi- subunit surveillance/effector complexes, allowing ...
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
... II CRISPR systems, a CRISPR RNA (crRNA), which contains sequence complemen- tary to invading virus or plasmid DNA, and a trans-activating CRISPR RNA (tracrRNA) interact with a ...
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Cutting it Right: Plasticity and Strategy of CRISPR RNA Specific Nucleases
... with CRISPR-associated (Cas) proteins. This RNA mediated defence system confers resistance against the invading mobile genetic elements such as phages and ...The CRISPR-Cas system operates by forming ...
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Complete genome sequence of Thermotoga sp. strain RQ7
... non-coding CRISPR RNA and a set of CRISPR-associated proteins to target invading nucleic acid, including both DNA and ...of CRISPR arrays in ...eight CRISPR loci, #1 and #3 are the ones ...
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Use of RNA-Protein Complexes for Genome Editing in Non-albicans Candida Species
... the CRISPR genome modification method tested here, the CRISPR machinery that is cotransformed with the gene deletion construct cleaves the chromosome at a site between the regions where homologous ...
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A Review of CRISPR-Based Genome Editing: Survival, Evolution and Challenges
... that CRISPR/Cas system involves the defense against invading selfish elements (Mojica et ...unique CRISPR spacer sequences which are almost identical to those of viral or plasmid gene fragments (Bolotin et ...
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Engineering CRISPR guide RNA riboswitches for in vivo applications
... system CRISPR (clustered regularly interspaced short palindromic repeats (CRISPR) offers a unique and highly effective toolbox for targeted genomic engineering, providing a versatile system to virtually ...
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Multiplexed CRISPR/Cas9 gene knockout with simple crRNA:tracrRNA co-transfection
... a CRISPR/Cas9 gene KO method that is analogous to siRNA transfection, we reasoned that it should be possible to transfect the CRISPR RNA ele- ments into cells that stably express the Cas9 apoenzyme ...
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CRISPR Cas Biology and Its Application to Infectious Diseases
... accompanying CRISPR-associated (Cas) proteins has resulted in rapidly expanding research and clinical ...The CRISPR locus was first identified in 1987, when a genetic structure containing 5 highly homologous ...
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CRISPR Cpf1 proteins: structure, function and implications for genome editing
... and CRISPR‑associated (Cas) protein, as components of microbial adaptive immune system, allows biologists to edit genomic DNA in a precise and specific ...way. CRISPR‑Cas systems are classified into two ...
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A New Group of Phage Anti CRISPR Genes Inhibits the Type I E CRISPR Cas System of Pseudomonas aeruginosa
... I-E CRISPR-Cas system of strain SMC4386 was isolated, we were able to test di- rectly the type I-E anti-CRISPR activity of individual genes from phage anti-CRISPR regions by expressing them from ...
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The C-Rel Transcription Factor Controls Metabolism And Proliferation Of Human T Cells
... investigate the function and regulation of c-Rel in human T cells, we generated c-Rel knockout human T cell line Jurkat cells using the CRISPR/Cas9 system and analyzed the resulting phenotype. Our results revealed ...
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HIV-1: tackling the obstacles that limit the effectiveness of CRISPR-Cas9 gene editing of the T cell co-receptor CCR5 Tatiana Hillman 1https://orcid.org/0000-0002-7539-0512
... PREVENTING CRISPR CAS9-SGRNA OFFTARGET MODIFICATIONS Hu et ...engineered CRISPR-Cas9 to delete sites in the HIV-1 LTR U3 region, ceasing gene expression and replication within HIV-1 ...guide RNA ...
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Evolution of RNA- and DNA-guided antivirus defense systems in prokaryotes and eukaryotes: common ancestry vs convergence
... of CRISPR-Cas from eukaryotes. CRISPR- Cas is not the only prokaryotic defense system that is missing in eukaryotes: also absent are RM and TA modules ...to CRISPR-Cas as well even though it is not a ...
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RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes
... disease. CRISPR-Cas9 is a scalable and effective new technology that can be used to knock out individual gene expression in large scale at the gene level and do so with minimal off-target ...remain, ...
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Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR Cas adaptive immunity
... general RNA processing factor in bacteria—was recruited to cleave tracrRNA and pre-crRNA upon base pairing and that sta- bilization of the duplex RNA by the protein Cas9 was required in the process ...
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CRISPR Interference of a Clonally Variant GC Rich Noncoding RNA Family Leads to General Repression of var Genes in Plasmodium falciparum
... Recently, RNA FISH was used to demonstrate the physical association of GC-rich ncRNAs with the expression site of central and subte- lomeric var genes ...end RNA sequencing (RNA-seq) analysis at 12 ...
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DNA Nanoclew-Mediated Drug Delivery.
... small RNA therapeutics into multi-lamellar liposome-like structure with sizes ranging from 35 nm to 60 ...the RNA cargo into endothelial cell as well as solid tumors in the lung [209, ...
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Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system
... After the preliminary establishment of the CRISPR/ Cas9 system, we attempted different genome editing approaches in P. putida KT2440. We showed that an N20 sequence could obtain a similar high mutation rate ...
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CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif
... by CRISPR/Cas9, was conducted using the on-target sites of the sgRNAs as the positive control [17], and the cleavage activity of 17-, 18-, 19-, and 20-nt sgRNAs ranged from ...
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