Culture and serotyping
Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples
... Karin Elberse 1* , Suzan van Mens 2 , Amelieke J Cremers 4,5 , Sabine CA Meijvis 3 , Bart Vlaminckx 2 , Marien I de Jonge 4,5 , Jacques F Meis 6,7 , Cornelis Blauwendraat 1 , Ingrid van de Pol 1 and Leo M Schouls 1 ...
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Competitive Inhibition Flow Analysis Assay for the Non-Culture-Based Detection and Serotyping of Pneumococcal Capsular Polysaccharide
... Received 4 August 2008/Returned for modification 30 September 2008/Accepted 5 December 2008 Traditional confirmation procedures for the identification of a pneumococcal serotype require an isolate. ...
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Reactivities of serotyping monoclonal antibodies with culture adapted human rotaviruses
... The monoclonal antibodies included in the compara- tive serotyping study were all found to be serotype specific when tested against laboratory strains representative of the six human rot[r] ...
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Marrow grafts between canine siblings matched by serotyping and mixed leukocyte culture
... Marrow grafts were carried out between 16 canine sibling donor-recipient pairs. The pairs were matched by serological histocompatibility testing and were nonreactive in a one-way mixed leukocyte culture. ...
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Capsular Serotyping and Antimicrobial Drug Resistance of
... Preparation of S. aureus Cowan 1 Strain Reagent: To find out K antigens of K.pneumoniae by Co-A method using S. aureus Cowan 1 strain, the following procedure was used. Culture of S. aureus Cowan 1 strain was ...
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Serotyping of Cryptococcus neoformans by dot enzyme assay
... Georgia 30303, 1 and Department of Microbiology, University of Nevada, Reno, Nevada 89557 2 Received 7 July 1995/Returned for modification 25 August 1995/Accepted 15 November 1995 A method is described for the ...
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Multilocus sequence typing as a replacement for serotyping in salmonella enterica
... inaccurate serotyping, which has plagued several percent of database entries from all the laboratories involved in this project, as well as in ring trials for testing laboratory accuracy ...original culture ...
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Serotyping of Adenoviruses on Conjunctival Scrapings by PCR and Sequence Analysis
... of serotyping by the culture isolation-NT, PCR-RFLP, and PCR- sequence methods for 31 clinical specimens are shown in Table ...the culture isolation-NT and PCR- RFLP analysis conflicted, the homology ...
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Comprehensive Detection and Serotyping of Human Adenoviruses by PCR and Sequencing
... Amplicons were successfully obtained from ATCC strains VR1501 and VR1502, candidate strains for serotypes 50 and 51, respectively (15), and their sequences were compared by phylogenetic analysis with those of the ...
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Challenges of Using Molecular Serotyping for Surveillance of Pneumococcal Disease
... groups. This is problematic for serotype-specific analyses such as capsular-based vaccine effectiveness studies (24) and serotype as- sociation studies (4, 25), where the separation of individual sero- types is ...
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Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
... Dis. 148:1136-1159, 1983). To validate the new assay, we examined 495 clinical isolates of pneumococci obtained in Brazil, Denmark, and Mexico. Pneumococci were serotyped by the Neufeld test in their countries of origin, ...
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Development of a DNA Microarray for Detection and Serotyping of Enterotoxigenic Escherichia coli
... Enterotoxigenic Escherichia coli (ETEC) is a common pathogen worldwide causing infectious diarrhea, especially traveler’s diarrhea. Traditional physiological assays, immunoassays, and PCR-based methods for the detection ...
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Serotyping Techniques
... If the person is a secretor, then there will be no agglutination of the RBC’s because all of the antibodies will have been neutralized by the blood group antigens in the saliva. If a pe[r] ...
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Comparison of the PremiTest and xMAP Salmonella serotyping assays and classical serotyping for determination of Salmonella serovars
... easily be incorporated into any laboratory that does serotyping. As more isolates are tested, information could be added to the PTS database and released as simple software updates. There are some issues that need ...
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O serotyping Providencia alcalifaciens
... For further typing we therefore sepa- rated the Providencia 0-serotyping (6, 15) scheme according to species, and only isolates that are untypable in antisera against strains of.. their [r] ...
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Serotyping of non cholera vibrios
... The remainder of the plate culture was used as the source of the organisms for the slide agglutination test with the typing sera.. The initial test was performed.[r] ...
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Serotyping Cryptococcus neoformans by immunofluorescence
... Antisera obtained from all four rabbits immunized with the B551 antigen (serotype A), when labeled, intensely stained cells of the homologous strain.. One conjugate, prepared from serum [r] ...
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Serotyping of Ureaplasma urealyticum by immunoperoxidase assay
... Routinely, we used as our control a single plate with colonies of mixed serotypes, reacted with homologous antiserum and conjugate plus DAB.. This permitted a valuable comparison of stai[r] ...
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O serotyping scheme for Enterobacter cloacae
... were agglutinated by at least one unabsorbed serum to a titer of 160, but no distinct type reactions were observed. Furthermore, one-half of the low titer reactions obtained with NT cult[r] ...
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Coagglutination test for serotyping Pasteurella haemolytica
... Department of Microbiology and Infectious Diseases, University of Veterinary Science, Budapest H-1581, Hungary Received 11 July 1995/Returned for modification 15 September 1995/Accepted 15 November 1995 A coagglutination ...
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