Cyanogen bromide
Cyanogen bromide digestion of the avian myeloblastosis virus pp19 protein: isolation of an amino-terminal peptide that binds to viral RNA.
... In an attempt to resolve a cyanogen bromide peptide which retained the ability to bind to viral I 01 IC RNA, the cyanogen bromide-treated ppl9 protein was chromatographed on an AMV 60S R[r] ...
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Flash preparation of carbenoids: A different performance of cyanogen bromide
... Synthesis of 2, Method A: In a 25 mL round bottom flask equipped by a magnetically stirrer, dissolved 0.12 g (0.96 mmol) cyanogen bromide (BrCN) in 4 mL ethanol at 0 °C. Then separately, 0.15 g (0.96 mmol) ...
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Structure of the membrane protein of influenza virus. I. Isolation and characterization of cyanogen bromide cleavage products.
... 30, 1979 MEMBRANE PROTEIN OF INFLUENZA VIRUS 761 Calculation of the amino acid composition of the recycled P6/2 peptide on the basis of one homoserine residue revealed a content of about[r] ...
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Fine-structure analyses of lipid-protein and protein-protein interactions of gag protein p19 of the avian sarcoma and leukemia viruses by cyanogen bromide mapping.
... To determine the fragment compositions of the six products derived from p19, we applied a two-dimensional mapping analysis described in detail previously for the gag protein of Gazdar mu[r] ...
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Separation of cyanogen bromide-cleaved peptides of the vesicular stomatitis virus glycoprotein and analysis of their carbohydrate content.
... In addition, six other peptides contained small amounts of sialic acid, fucose, and mannose, indicating that the glycoprotein contains more carbohydrate chains than the two major ones wh[r] ...
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Chromatographic Separation and Antigenic Analysis of Proteins of the Oncornaviruses
... The values thus obtained for methionine content are consistent with the numbers of peptide fragments obtained by cyanogen bromide cleavage of Downloaded from http://jvi.asm.org/ on Novem[r] ...
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Collagen induced arthritis in common marmosets: a new nonhuman primate model for chronic arthritis
... A detailed analysis of collagen type II CII-specific T-cell responses against a cyanogen bromide fragment 11 CB11-derived overlapping peptide set see Table 3 was performed on the day of [r] ...
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Epitope mapping of the von Willebrand factor subunit distinguishes fragments present in normal and type IIA von Willebrand disease from those generated by plasmin
... with cyanogen bromide and tryptic fragments of known carboxy and/or amino termini, showed that in normal and IIA von Willebrand disease (vWD) plasmas the 140 kD fragment was derived from the amino-terminal ...
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Purification of an IgA Monoclonal Antibody Specific for the Acr Protein of Mycobacterium tuberculosis by Immunoaffinity Chromatography
... Methods: To minimise the number of chromatographic steps, we proposed to employ immunoaffinity chromatography using the Acr protein of MTB as a specific ligand for this mAb. For this purpose, the HspX gene was cloned and ...
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Temperate Bacteriophage Which Causes the Production of a New Major Outer Membrane Protein by Escherichia coli
... Cyanogen bromide peptides from the "A" protein from a mixture of outer membrane proteins from strain J-5 solid line and from a culture of strain 1485F+ lysogenic for phage PA-2 dashed li[r] ...
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Characteristics and regulation of interaction of avian retrovirus pp12 protein with viral RNA.
... The Kapp was calculated from Scatchard plots for the binding of AMV or RSV Pr-C ppl2 proteins each 0.08 p.g, the amino-terminal cyanogen bromide peptide of RSV Pr-C ppl2 1.6 ,ug, or the [r] ...
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Transferrin Polymorphism in Pigeons
... This is based on the analysis of cyanogen bromide fragments of chicken ovotransferrin 41, in which molecular weight and charge similarities of the pairs of the fragments suggested some d[r] ...
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TWO DISTINCT PANCREATIC AMYLASE GENES ARE ACTIVE IN YBR MICE
... I n addition, the extra acidic residue in A, can account for the lower iso- electric point of the intact A, isozyme (Figure I) and of its cyanogen bromide fragment (Figur[r] ...
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Molecular cloning of the human Goodpasture antigen demonstrates it to be the alpha 3 chain of type IV collagen
... or cyanogen bromide cleavage, and used to deduce redundant oligonucleotides for use in the polymerase chain reaction on cDNA derived from sheep renal ...
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In silico proteome analysis to facilitate proteomics experiments using mass spectrometry
... A fundamental operation of proteomics is to identify pro- teins. For most high-throughput applications, proteins are cleaved with site-specific reagents, for example cyanogen bromide (CNBr) or proteases ...
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Removal of bromide from aqueous solutions using the UV/ZnO process based on the multivariate analysis model
... of bromide removal is at neutral pH or equal to 7 (approximately ...of bromide species are created by increasing the bromide concentration and because ZnO nanoparticles have more positive charges at ...
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Genetic variability for cyanogen and trypsin inhibitor contents in sorghum (Sorghum bicolor (L.) Moench)
... When the 33 selected genotypes were reassessed for cyanogen levels, the mean values obtained were near to the previous results. The correlation between the two sets of values was (r = 0.65, p < 0.01) highly ...
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Modeling Of Solar Absorption Air Conditioning System Applied To An Office Building In Morocco.
... necessary for its vaporization from the water to be cooled. The saturated steam then leaves the evaporator (state 4) and feeds the absorber where the concentrated lithium bromide solution flows, which dilutes by ...
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Effects of processing methods on antinutrient composition of seeds from a wild legume bauhinia Petersiana
... reported to be highly toxic under experimental conditions, acute saponin poisoning is rare both in animals and man (Nwinuka et al., 2005). As shown in Table 1, the levels of cyanogen glycosides in B. petersiana ...
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Application of superbase catalyst γ Al2O3/NaOH/Na for prenylation reaction of resveratrol (3,5,4' trihydroxystilbene)
... The synthesis of prenylated resveratrol compound will be conducted by reaction between resveratrol 3,5,4'-trihydroxystilbene and prenyl bromide 3,3-dimethyl allyl bromide using heterogen[r] ...
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