Deletion strains
Role for RNA-Binding Proteins Implicated in Pathogenic Development of Ustilago maydis
... ⌬ strains are reduced in filamentous growth and viru- ...⌬ strains were slightly affected in mating (Table ...rrm4 deletion strains were impaired in mating ...respective strains were ...
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Functional Analysis of Sterol Transporter Orthologues in the Filamentous Fungus Aspergillus nidulans
... gene deletion strains were constructed (see Materials and ...oshB deletion strain was smaller than that of the wild type (70%), the colonies of the oshC and oshD deletion strains were ...
14
Molecular Characterization of Propolis-Induced Cell Death in Saccharomyces cerevisiae
... RAV1, and SOP4) involved in the assembly of the yeast V-AT- Pase were deleted, the corresponding yeast deletion strains became more sensitive to propolis (Fig. 9A). Recently, Rosado et al. (67) developed a ...
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Chitosan, the Deacetylated Form of Chitin, Is Necessary for Cell Wall Integrity in Cryptococcus neoformans
... Chitosan-deficient strains are sensitive to select cell wall ...deacetylase deletion strains on five cell wall inhibi- tors that assess different aspects of cell wall ...deacetylase deletion ...
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Cch1 and Mid1 Are Functionally Required for Vegetative Growth under Low-Calcium Conditions in the Phytopathogenic Ascomycete Botrytis cinerea
... gene deletion strains and the ⌬ cch1 ⌬ mid1 double deletion mutant in comparison to the wild-type strain ...(A) Strains were grown on complete medium (CM). (Left) Strains were cultured ...
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Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485
... pfl deletion strains, we think this is likely the case in ...pfl deletion strains was restored by the addi- tion of serine, suggesting that C1 units are transferred from serine to ...
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The Transcription Factor StuA Regulates Central Carbon Metabolism, Mycotoxin Production, and Effector Gene Expression in the Wheat Pathogen Stagonospora nodorum
... An analysis of the factor loadings from the PCA score plot and subsequent analysis of variance identified 62 peaks from the GC-MS traces that were significantly differentially abun- dant when comparing normalized peak ...
9
Roles of the Glyoxylate and Methylcitrate Cycles in Sexual Development and Virulence in the Cereal Pathogen Gibberella zeae
... the deletion strains on basal medium containing only nonfermentable carbon sources, such as sodium acetate, ethanol, C 3 propionic acid, C 12 fatty acid (monolaurate; Tween 20), and C 18 fatty acids (Tween ...
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Minor Isozymes Tailor Yeast Metabolism to Carbon Availability
... resulting strains measured (3, 11, ...double deletion strains (17) or triple deletion strains (18), is that they have been conducted under only a few environmental conditions, mainly ...
19
Ribosome Deficiency Protects Against ER Stress in Saccharomyces cerevisiae
... of strains in the ORF deletion collection, we regenerated the entire set of haploid ribosomal protein gene deletion strains in a clean genetic ...new strains were used to create double ...
44
Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans
... Conidiation in Aspergillus nidulans is induced by exposure to red light but can also be induced by blue light in certain mutant strains. We have isolated a mutation in the fluG gene that abolishes responsiveness ...
10
REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA II. A MUTATION WHICH CAUSES HOT SPOT ACTIVITY
... Thus, HENDERSON’S mutation should have been present in the deletion strains which exhibited recombinational hot spot activity near the right end of the chromosome, and the [r] ...
9
Transcriptional activator Cat8 is involved in regulation of xylose alcoholic fermentation in the thermotolerant yeast Ogataea (Hansenula) polymorpha
... The following strains of O. polymorpha were used: NCYC495 leu1-1 (wild-type strain), 2EtOH/XYL1m/ XYL2/XYL3/BrPA (designated as BEP from best etha- nol producer) which is advanced ethanol producer from xylose ...
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Establishment of markerless gene deletion tools in thermophilic Bacillus smithii and construction of multiple mutant strains
... knockout strains, the counter-selection was applied by growing the colo- nies containing the mixed genotype of single and double crossovers overnight in 10 mL LB2 at 55°C, after which dilution series were plated ...
12
NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level
... (double deletion mutXR) grows to ...(double deletion no XR), it would take 27 batch cultivations of 48 hours each before one TMB4144 cell would outgrow 10 9 TMB4140 cells by a factor of 1:100 (according to ...
12
THE GALACTOSE OPERON OF E. COLI K-12. I. STRUCTURAL AND PLEIOTROPIC MUTATIONS OF THE OPERON
... They were then assayed for transduction of strains carrying both a n amber or ochre suppressor and the large S165 deletion which removes the entire epimerase and transferase [r] ...
17
Interaction among Btn1p, Btn2p, and Ist2p Reveals Potential Interplay among the Vacuole, Amino Acid Levels, and Ion Homeostasis in the Yeast Saccharomyces cerevisiae
... A deletion in Btn2p (btn2 ⌬ strains) results in a failure to correctly localize Ist2p, and strains lacking Btn2p and Ist2p (btn2 ⌬ ist2 ⌬ strains) are unable to grow in the presence of ...⌬ ...
8
Transcription Factors Pcr1 and Atf1 Have Distinct Roles in Stress- and Sty1-Dependent Gene Regulation
... promoters. Strains JM1821 (atf1-HA6H), MS65 (pcr1-HA) (both strains tagged at the chromosomal loci), or wild-type 972 with no plasmid (no tag) were grown, formaldehyde extracts were obtained, and chromatin ...
10
Regulation of RNA stability by terminal nucleotidyltransferases
... Although phosphorylation of S116 inhibits Gld2 catalytic activity, RNA binding affinity was unperturbed, suggesting Gld2 phosphorylated at S116 binds the RNA substrate in a non-productive conformation. The crystal ...
185
Comparative Genomics Using Microarrays Reveals Divergence and Loss of Virulence-Associated Genes in Host-Specific Strains of the Insect Pathogen Metarhizium anisopliae
... specialist strains kill their host slowly (8). Pre- sumably, strains that are not specifically adapted to subvert/ avoid/overcome the immune response of a particular insect are best served by achieving a ...
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