Delta antigen
Construction and Eukaryotic Expression of Recombinant Large Hepatitis Delta Antigen
... Delta antigen was detected by Western blot analysis of cell lysates 2 days post-transfection. Cells were lysed directly with 400 µl of sample buffer (80 mM Tris-HCl, pH 6.8, 2% sodium dodecyl sulfate (SDS), ...
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Large Hepatitis Delta Antigen Is Not a Suppressor of Hepatitis Delta Virus RNA Synthesis once RNA Replication Is Established
... hepatitis delta virus (HDV) replication is a likely prerequisite in the establishment of chronic infections and is thought to be mediated by the intracellular accumulation of large hepatitis delta ...
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A novel form of hepatitis delta antigen.
... the delta antigen, was raised in a rabbit by inoculation with delta antigen which had been prepared in Escherichia ...the delta antigen (residues 197 to 211), and was shown to ...
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Multimerization of Hepatitis Delta Antigen Is a Critical Determinant of RNA Binding Specificity
... Hepatitis delta virus (HDV) RNA forms an unbranched rod structure that is associated with hepatitis delta antigen (HDAg) in cells replicating ...
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Hepatitis Delta Antigen Regulates mRNA and Antigenome RNA Levels during Hepatitis Delta Virus Replication
... Hepatitis delta virus (HDV) is a satellite of hepatitis B virus that in- creases the severity of acute and chronic liver ...hepatitis delta antigen ...
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Positive Selection of Hepatitis Delta Antigen in Chronic Hepatitis D Patients
... Liver disease may become ameliorated in some patients with chronic hepatitis D virus (HDV) infection. We present here a study based on longitudinal sampling to investigate the viral dynamics in chronic HDV infection. We ...
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Hepatitis Delta Antigen Requires a Minimum Length of the Hepatitis Delta Virus Unbranched Rod RNA Structure for Binding
... Hepatitis delta virus (HDV) is a subviral pathogen that increases the severity of liver disease caused by hepatitis B ...hepatitis delta antigen (HDAg), in infected cells; however, the nature of the ...
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Arginine-Rich Motifs Are Not Required for Hepatitis Delta Virus RNA Binding Activity of the Hepatitis Delta Antigen
... Hepatitis delta virus (HDV) replication and packaging require interactions between the unbranched rodlike structure of HDV RNA and hepatitis delta antigen (HDAg), a basic, disordered, oligomeric ...
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Genotype-Specific Complementation of Hepatitis Delta Virus RNA Replication by Hepatitis Delta Antigen
... hepatitis delta virus (HDV) isolates have focused principally on phylogenetic analysis of sequences, which vary by 30 to 40% among three genotypes and about 10 to 15% among isolates of the same ...hepatitis ...
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The Conserved Serine 177 in the Delta Antigen of Hepatitis Delta Virus Is One Putative Phosphorylation Site and Is Required for Efficient Viral RNA Replication
... Hepatitis delta virus (HDV) is a defective virus and requires its helper virus, hepatitis B virus (HBV), to supply the enve- lope proteins for viral assembly (36, ...hepatitis delta antigen ...
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Hepatitis Delta Antigen Mediates the Nuclear Import of Hepatitis Delta Virus RNA
... Hepatitis delta virus (HDV) RNA replicates in the nuclei of virus-infected ...hepatitis delta antigen (HDAg), which is the only protein encoded by HDV RNA, the HDV RNA was translocated into the ...
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Oligomerization of hepatitis delta antigen is required for both the trans-activating and trans-dominant inhibitory activities of the delta antigen.
... 6641 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Two forms of hepatitis delta antigen HDAg have different roles in the replication cycle of hepatitis delta virus HDV[r] ...
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ERK1/2-Mediated Phosphorylation of Small Hepatitis Delta Antigen at Serine 177 Enhances Hepatitis Delta Virus Antigenomic RNA Replication
... Hepatitis delta virus (HDV) is a subviral pathogen that can self-replicate but relies on its helper hepatitis B virus to pro- vide envelope proteins for HDV infection and virion assembly ...hepatitis delta ...
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Activation of Heterologous Gene Expression by the Large Isoform of Hepatitis Delta Antigen
... Hepatitis delta virus (HDV) is an RNA virus that requires coinfection with hepatitis B virus (HBV) to complete its life ...atitis delta antigen (HDAg), is encoded by the genome (3, 38), and two ...
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Hepatitis delta virus: protein composition of delta antigen and its hepatitis B virus-derived envelope.
... 945 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hepatitis delta virus HDV-associated particles were purified from the serum of an experimentally infected chimpanzee[r] ...
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Enzyme linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen associated delta antigen
... An HBsAg-positive serum characterized as containing hepatitis B e1 antigen HBe,Ag and hepatitis B e2 antigen HBe2Ag by munodiffusion and reacting in HBeAg-RIA at 1:2,560 dilutions 1 was [r] ...
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Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen.
... DISCUSSION We have observed that transfection of both liver HuH7 and nonliver COS7 cells with a DNA trimer of the HDV genome led to an apparent replication of the HDV RNA genome.. There [r] ...
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A single antigenomic open reading frame of the hepatitis delta virus encodes the epitope(s) of both hepatitis delta antigen polypeptides p24 delta and p27 delta.
... C Immunoblots of HDAg-positive liver extracts incubated with the following antisera: lanes 1 and 2, a 1:1,000 dilution of an HDV antiserum preabsorbed with extracts of yeast cultures exp[r] ...
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Large Hepatitis Delta Antigen Is a Novel Clathrin Adaptor-Like Protein
... In this study, we intend to examine possible interactions between cytoplasm-localized HDAg-L and host factors and to elucidate possible mechanisms of HDAg-L involvement in the viral life cycle and the pathogenesis of HDV ...
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Functional motifs of delta antigen essential for RNA binding and replication of hepatitis delta virus.
... Lanes W, M, N, and MN contain the wild-type and mutants which are mutated in the middle domain, near the N terminus, or both of the leucine repeats for the small HDAg, respectively.. Pla[r] ...
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