density-gradient centrifugation
Inter center variation in the efficiency of sperm DNA damage reduction following density gradient centrifugation
... and density gradient centrifugation (DGC) at the time equivalent to that of fertilization and then cryopreserved as per the NSS to be analyzed at a different ...
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Density Gradient Centrifugation Studies on Lymphocytic Choriomeningitis Virus and on Viral Ribonucleic Acid
... 414-420 Copyright © 1970 American Society for Microbiology Density Gradient Centrifugation Studies on Lymphocytic Choriomeningitis Virus and on Viral Ribonucleic Acid Received for public[r] ...
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Solid phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test
... Solid-Phase Radioimmunoassay of Rubella Virus Immunoglobulin M Antibodies: Comparison with Sucrose Density Gradient Centrifugation Test OLLI H.. VILJANEN, AND KAISA GRANFORS Departments [r] ...
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The effect of supernatant product of adipose tissue derived mesenchymal stem cells and density gradient centrifugation preparation methods on pregnancy in intrauterine insemination cycles: An RCT
... Currently, density gradient centrifugation (DGC) is one of the routine methods in assisted reproduction technique ...removing density medium, the obtained soft pellet containing qualitative ...
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Density Gradient Centrifugation of Rubella Virus
... As EDTA can be seen, the virus obtained from the heavier 1.18 g/cm3 band after 18 hr of centrifugation was B Virus centrifuged in sucrose found on recentrifugation to band at the lighter[r] ...
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Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
... By using rate-zonal centrifugation followed by tional Institutes of Health, USPHS, Minimum Reisopycnic banding, we previously demonstrated quirements: Influenza Virus Vaccines, Types A a[r] ...
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Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid phase reverse immunosorbent test with sucrose density gradient centrifugation
... Diagnosis of Recent Rubella Virus Infection by Demonstration of Specific Immunoglobulin M Antibodies: Comparison of Solid-Phase Reverse Immunosorbent Test with Sucrose Density Gradient C[r] ...
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Sperm quality after density gradient centrifugation with three commercially available media: a controlled trial
... by density gradient and swim up methods and found that sperm preparation by density gradient using Percoll re- sulted in higher percentage of DNA damage compared to those separated by swim-up ...
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Fractionation of Eastern Equine Encephalitis Virus by Density Gradient Centrifugation in CsCl
... It was also recently reported that EEE virus, in the form of a 20% mouse brain suspension, banded at 1.21 to 1.23 g/cm3 in CsCl and that no hemagglutinin was detected in the upper region[r] ...
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Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
... Sperm cells (3 × 10 6 ) were washed twice with 9% (v/v) sucrose and solubilized with 250 μl of DeStreak® rehy- dration solution (GE Healthcare, Uppsala, Sweden) with the addition of 1% (v/v) IPG 3–11 solution (GE ...
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Effect of combined density gradient centrifugation on X- and Y- sperm separation and chromatin integrity
... study, centrifugation with PureSperm gradients led to a slight but nonsignificant enrichment in ...albumin gradient centrifugation, we obtained a slight but nonsignificant enrichment of Y- ...
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A COST-SAVING TECHNIQUE OF HUMAN SOMATIC LIVER STEM CELLS ISOLATION FOR EX VIVO PROPAGATION AND CHARACTERIZATION
... Aim: The study of stem cell biology provides insight into mechanisms that regulate tissue maintenance in normal disease status. With an impressive rate of epithelial renewal driven by a pool of multi potent stem cells ...
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Reovirus-directed Ribonucleic Acid Synthesis in Infected L Cells
... Reovirus was purified from infected cells by genetron treatment and CsCl equilibrium density gradient centrifugation, and double-stranded RNA was extracted from virus with phenol 33.. Be[r] ...
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Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing
... Results: Oocysts from 21 human stool specimens were separated from other stool components using discontinuous density gradient centrifugation and purified further by flow cytometry. Genomic DNA was ...
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Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles
... Having shown that coagulation pathways and four coagulation proteins were over-represented in the (V)LDL precipitate that contains EVs (Figures 1 and 2), we further investigated whether these coagulation proteins were ...
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Structural and growth characteristics of infectious bursal disease virus.
... Virus was purified from the BF of birds infected with field material by density gradient centrifugation in sucrose and CsCl.. The 7.5% polyacrylamide gel contained 5 M urea; the buffer w[r] ...
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Ultrastructure and chemical analysis of Campylobacter pylori flagella
... b SDS-PAGE of the flagellar extract before sucrose density gradient centrifugation lane s and of fractions as indicated in panel a lanes a,, a2, b.. Peak b contained the bulk of flagella[r] ...
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Part I: The abortive infection of bacteriophage øx174 at low temperatures. Part II: The early stages in the process of infection by bacteriophage øx174
... CsCl equilibrium density gradient centrifugation (mean density. 37 g/ml.) of the material which remained associated w i th the cells after the elution treatment. infected cell lysat[r] ...
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A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
... Ethidium bromide-stained agarose gel A and autoradiogram B of the dsRNAs contained in purified VLPs from strain RE455 by cesium chloride density gradient centrifugation and the products [r] ...
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An acid transporting enzyme in human gastric mucosa
... by density gradient centrifugation yielded a vesicular membrane preparation free of mitochondrial markers, containing a K+-activated, ouabain-insensitive ATPase with an activity of ...
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