Designing and using splice switching oligonucleotides
Targeting the ERG oncogene with splice-switching oligonucleotides as a novel therapeutic strategy in prostate cancer
... with splice-switching oligonucleotides (SSOs) resulted in decreased cell proliferation, migration and increased apoptosis 14 confirming that exon 7b contributes to ERG’s oncogenic ...
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Systematic approach to developing splice modulating antisense oligonucleotides
... different splice motifs and sites are recognised in a developmental and/or tissue-specific manner, contributes to genetic plasticity and diversity of gene ...antisense oligonucleotides to alter splicing of ...
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Reduction of integrin alpha 4 activity through splice modulating antisense oligonucleotides
... effective splice switching/suppression can be ...selection using splice modulating AOs can offer new therapeutic avenues for serious human ...
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Splice correcting oligonucleotides restore BTK function in X linked agammaglobulinemia model
... antisense, splice-correcting oligonucleotides (SCOs) targeting mutated BTK transcripts for treating ...optimized using 2′-O-methyl, locked nucleic acid, or phosphorodiamidate morpholino ...mutated, ...
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LOGIC DESIGNING AND SWITCHING THEORY BOOK
... A=B=1; C=0 O/p = 1 Now we have to precisely examine the input and the logical gates we have to satisfy the condition of input. It is known that the functionality of AND gate is that it provides logical output HIGH when ...
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Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice switching of MYO1B
... 32). Using EGFP fusion proteins, we pro- vided evidence that EGFP-fused MYO1B-fl localized mainly on the cytomembrane, while EGFP-fused MYO1B-t was dispersed in the ...
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Antisense oligonucleotides
... Methylation of cytosines at the 59 position is one way to increase ASO specificity. For example, all cytosines in an ASO against SOD1 included the 59-methylcytosine modification. 5 This inclusion can enhance base pairing ...
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Novel viral vectors utilizing intron splice switching to activate genome rescue, expression and replication in targeted cells
... of splice-switch technology were developed nearly two decades ago and since then the technology and its variants, such as exon skipping, have been envi- sioned as potent approaches to the treatment of several ...
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CD44 splice isoform switching in human and mouse epithelium is essential for epithelial mesenchymal transition and breast cancer progression
... vivo. Using a mouse model of breast tumor formation and recur- rence, we identified a drastic change in CD44 isoform expression from CD44v in the epithelial primary tumors to CD44s in the recurrent tumors that ...
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Stochastic Switching Using OpenFlow
... The chapter was started by designing simple scenarios such as scenarios one and two. While further studying OpenFlow features, it has come to our attention that the main purpose of introducing SELECT method of ...
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Designing an Incapacitator Using LED
... 3.1. Generating the Light Patterns The light patterns to incapacitate the human being are generated in the strobing mode of the BSSL. The current generated by the buck type LED drivers is switched on and off at 500kHz . ...
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Designing of Hybrid Automatic Solar Charging System with Power Controller for Switching Power Utilization
... In Solar control unit that the charge controller is the main regulator to increase the lifetime of the battery. And power controller controls the power flow from inputs and outputs. This research deals with solar energy ...
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Overview - of Seismic Resistance of Railway Steel Trusses Bridges Using Splice Connection
... general, designing a structure that minimizes a total cost, including not only construction but also repairing costs after severe earthquake, requires considerable number of calculations in a trial-and-error ...
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Chromosome-Specific Painting in Cucumis Species Using Bulked Oligonucleotides
... labeled using the tradi- tional hapten biotin or digoxigenin and thus the signals from these probes can be amplified using antibodies against biotin and ...
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Chemical Modifications Of Oligonucleotides
... Molecular beacons: probes that fluoresce upon hybridization. Salmonella enhances the antitumor effects of cisplatin on prostate cancer. RNA duplexes discussed in this paper in any meaningful fashion. Life laboratory ...
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Efficient assembly of very short oligonucleotides using T4 DNA Ligase
... Findings To address the high cost and limited efficiency of de novo gene synthesis we are exploring gene synthesis by ligation of deoxyribooligonucleotides (oligos), short enough to permit DNA assembly from a library of ...
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Gene splice site prediction using Artificial Neural Networks
... Finding splice sites in a particular gene The splice site indicators can be plotted for a single ...acceptor splice site indicators for the entire gene. The donor splice sites are marked ...
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Chemical Synthesis and Purification of Oligonucleotides
... of oligonucleotides remained labor intensive and inefficient throughout the 1970’s and into the early ...longer oligonucleotides and to make them much more ...
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Synthesis and properties of modified oligonucleotides
... synthetic oligonucleotides to regulate gene expres- sion has triggered the search for new oligonucleotide derivatives with improved therapeutic ...synthetic oligonucleotides com- plementary to the mRNA of a ...
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Design and Application of Photoactivatable Oligonucleotides
... Photoactivatable oligonucleotides are DNA or RNA molecules whose function is temporarily blocked or "caged" by a chemical moiety that can be activated by ...By using light to turn on activity, high spatial ...
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