Detergent screening for membrane protein crystallisation
Deconvolution of Membrane Protein-Detergent Complex Interactions
... between various greasy proteins and detergents. I toiled for over a year to generate a manuscript describing the single channel characteristics of four genetically engineered pores[23] (Chapter 2 of this thesis), ...
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A rapid and affordable screening platform for membrane protein trafficking
... MarsCy1-tagged protein targets are easily generated using common molecular biological techniques and in the multitude of proteins tested have not significantly impacted normal ...
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CiteSeerX — Detergent-mediated reconstitution of membrane proteins
... that membrane proteins insert into these structures from both surfaces, without the need of the hydrophilic IIA domain to cross the bilayer, leading to a random ...intermediate detergent/lipid ratios (stage ...
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High-Efficiency Screening of Monoclonal Antibodies for Membrane Protein Crystallography
... Fab-membrane protein complex and crystallization trial In the final round of screening, Fab fragments are ...test membrane protein-Fab complex formation without purifying the Fab ...the ...
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Tools to Ease the Choice and Design of Protein Crystallisation Experiments
... fine screening and additive screening methods of optimisation rely on the user having a clear understanding of patterns in the outcomes of previous crystallisation ...given protein, and what ...
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Hepatitis C Virus Core Protein Associates with Detergent-Resistant Membranes Distinct from Classical Plasma Membrane Rafts
... core protein coding sequence into a mammalian expression vector, pEF6, and ex- ogenously expressed core protein in Huh7 ...to membrane flotation analysis as described for ...core protein ...
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Merging in-solution X-ray and neutron scattering data allows fine structural analysis of membrane-protein detergent complexes
... ntegral membrane proteins form the entry and exit routes for nutrients, metabolic waste and drugs in biological cells, and they are involved in key steps of signaling and energy ...on membrane proteins has ...
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Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.
... that membrane proteins account for approximately 20-30% of the genes coded in all sequenced genomes 1 , including the human genome 2 ...proteins, membrane proteins are highly under-represented in the ...
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Cell free expression and in meso crystallisation of an integral membrane kinase for structure determination
... a membrane mimetic, in part, because the aggregated protein so formed resembled the inclusion bodies that the protein, overexpressed in vivo, forms naturally and that has been used successfully for ...
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Assembly of Arenavirus Envelope Glycoprotein GPC in Detergent-Soluble Membrane Microdomains
... Received 24 April 2009/Accepted 8 July 2009 The family Arenaviridae includes a number of highly pathogenic viruses that are responsible for acute hemorrhagic fevers in humans. Genetic diversity among arenavirus species ...
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Follicle Stimulating Hormone Receptor Resides in Detergent Resistant Membrane Microdomains
... the protein caveolin. 13 Caveolin is a hair-pin like protein responsible for the unique shape of the caveolae, which is often described as a cave-like invagination in the cell membrane that is ...
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A method for detergent-free isolation of membrane proteins in their local lipid environment
... the protein that is being ...large membrane protein ...30 membrane proteins and have shown that proteins that contain up to 36 transmembrane helical elements can be ...their protein or ...
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Engineering a minimal G protein to facilitate crystallisation of G protein coupled receptors in their active conformation
... G protein from the receptor (Kuhn, 1981) and separation of G α from G βγ (Fung et ...G protein complexes are dif fi cult targets for structural studies due to their large size, conformational dynamics, and ...
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Using isoelectric point to determine the pH for initial protein crystallisation trials
... available protein is limited, it is im- 45 portant to identify suitable conditions in as few trials as possible and restricting screening to a particular pH range would reduce the num- ber ...
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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis
... of protein banding patterns relative to control extractions with CHAPS or SDS ...large protein with multiple transmembrane spanning domains [23] could be clearly distinguished in SDS extracts at an apparent ...
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Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive
... powder detergent, Aerial was used for detergent compatibility test of enzyme produced by the selected ...The detergent was heated at 100°C for 1hr to denature all enzymes found in the ...
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Detergent-Resistant Membrane Association of NS2 and E2 during Hepatitis C Virus Replication
... and membrane flotation assays to demonstrate that NS2 associates with detergent-resistant membranes (DRM) in a p7-dependent ...Core protein strongly associates with the DRM, recruitment of E2 and ...
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Discriminatory Role of Detergent-Resistant Membranes in the Dimerization and Endocytosis of Prostate-Specific Membrane Antigen
... different membrane environment that is reflected by their association with Tween 20-DRMs in the ER and prior to their exit from this organelle ...total protein from all fractions were loaded for each lane ...
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Characterising protein/detergent complexes by triple-detection size-exclusion chromatography
... However, membrane proteins usually reveal a migration behaviour different from that of soluble proteins, thus im- peding mass determination by standard SDS-PAGE ...for detergent-solubilised ...the ...
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Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
... integral membrane protein reconsti- tuted in ...other membrane proteins, similarly disposed, have been shown to be biologically ...native protein in the outer membrane of ...the ...
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