directed mutagenesis
Site-Directed Mutagenesis Studies of the Flexibility of the Distal Histidine in Dehaloperoxidase-Hemoglobin.
... Directed Mutagenesis Kit (Stratagene). The mutant plasmids were synthesized using 6XHis- tagged WT-DHP A plasmid as the template and upscaled on 2720 Thermo Cycler (Applied Biosystems, Inc) by the thermal ...
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“Natural Site Directed Mutagenesis” Might Exist in Eukaryotic Cells
... Site-directed mutagenesis refers to a molecular biology method that is used to make genetic alterations in the DNA sequence of the gene of interest [1] ...oligonucleotide-directed mutagenesis ...
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Heterologous expression and site directed mutagenesis of soluble methane monoxygenase
... The purpose of this investigation was to study the heterologous expression of soluble methane monooxygenase sMMO genes from Methylococcus capsula/us Bath and Methylosinus trichosporium O[r] ...
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Site-directed mutagenesis of a Saccharomyces cerevisiae mitochondrial translation initiation codon.
... Residual translation of the cox3-1 mutant mRNA was dependent upon the three nuclearly coded mRNA-specific activators PET494, PET54 and PET122, known from previous [r] ...
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Rapid sitrapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espD
... scanning mutagenesis kit from New England Biolabs, which is based on in vitro Tn7 random insertions ... mutagenesis kit was originally designed for site-directed mutagenesis targeting single ...
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Autoregulation of Epstein-Barr virus putative lytic switch gene BZLF1.
... The distinction between the elements involved in TPA induction and the ZIIIB domain was further demonstrated by site-directed mutagenesis, which affected ZEBRA binding and transactivatio[r] ...
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Regulation of Virus Neutralization and the Persistent Fraction by TRIM21
... prepared from 9C12 hybridoma cells (Developmental Studies Hybridoma Bank) and was subjected to PCR amplification by using a strategy based on that described previously by Li et al. (21). Mixtures of 5= degenerate primers ...
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Chemokine receptor CCR6 expression is regulated by miR 518a 5p in colorectal cancer cells
... site-directed mutagenesis spanning 4 bp introduced into the respective seed sequences is effectu- ally disrupting the interaction of miR-518a-5p with the 3′ UTR of ...
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Alternative splicing of a beta(4) subunit proline-rich motif regulates voltage-dependent gating and toxin block of Ca(v)2.1 Ca2+ channels
... N-terminal deletion and site-directed mutagenesis experiments revealed that the effects of alterna- tive splicing on toxin block and all aspects of gating could be attributed to a prolin[r] ...
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The scope and impact of post transcriptional gene regulation in plants microRNAs and the RNA binding proteome of Arabidopsis
... In plants, an alternative strategy to this has been the transgenic expression of miRNAresistant target genes, whereby using site-directed mutagenesis, silent mutations are introduced int[r] ...
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Mutations in the cytoplasmic tail of influenza A virus neuraminidase affect incorporation into virions.
... Transfectant virus was obtained with all cytoplasmic tail mutants generated by site-directed mutagenesis of the influenza A virus tail, except for the mutant resulting from substitution [r] ...
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Phosphorylation of specific serine residues within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro.
... By site-directed mutagenesis, we altered possible phosphate acceptor serines and threonines in domain I to a neutral amino acid alanine and then assayed the phosphorylated state of these[r] ...
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Characterization of the EYE2 Gene Required for Eyespot Assembly in Chlamydomonas reinhardtii
... plasmid-insertion mutagenesis screen yielded the eye2-2 allele, which allowed the isolation and characterization of the EYE2 ...Site-directed mutagenesis of the active site cysteines demonstrated ...
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Drosophila nonsense suppressors: functional analysis in Saccharomyces cerevisiae, Drosophila tissue culture cells and Drosophila melanogaster.
... Amber (UAG) and opal (UGA) nonsense suppressors were constructed by oligonucleotide site- directed mutagenesis of two Drosophila melanogaster leucine-tRNA genes and te[r] ...
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Directed Evolution of Terpene Synthases for Non Natural Substrates
... When superimposed, the three models (Fig. 6) indicated the H-α1 loop region was part of the active site, which in the closed conformation allowed the magnesium ion cluster, where the diphosphate binds, to move inwards. ...
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Redesign of a novel d-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of d-allulose
... On the other hand, protein engineering is a robust strategy for the creation of enzyme variants for novel biotechnological applications [17, 18]. Rational design and function modification based on structure–function ...
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Mobile genetic element encoded hypertolerance to copper protects Staphylococcus aureus from killing by host phagocytes
... To study the role of the copper-sensitive operon repressor CsoR in copper tolerance, site-directed mutagenesis was conducted on the csoR genes on the chromosomes of strain 14-2533T CC22 [r] ...
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Activated Alleles of the Schizosaccharomyces pombe gpa2+ Gα Gene Identify Residues Involved in GDP-GTP Exchange
... site-directed mutagenesis on a genomic clone of gpa2 (the original screen was carried out using a plasmid lacking the single gpa2 intron) and knocking the mu- tant allele into the gpa2 locus in strain MZP2 ...
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Nonsense suppression of the major rhodopsin gene of Drosophila.
... Drosophila strains harboring nonsense suppressor tRNAs have been made by site-directed mutagenesis and P element-mediated transformation of cloned tRNA genes (DOERIG [r] ...
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Biochemical and structural characterization of a DNA N 6-adenine methyltransferase from Helicobacter pylori
... Site-directed mutagenesis experiments identified residues D29 and E216 as crucial amino acids for cofactor binding and the methyl transfer activity of the enzyme, while P41, located in a highly flexible ...
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