DNA and histones
Neutrophil extracellular traps induce aggregation of washed human platelets independently of extracellular DNA and histones
... of DNA, histones and neutrophil proteases from neutrophils, was first demonstrated as a host defence against ...extracellular DNA and histones to platelet activation ...
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DNA polymerase interacts with H3-H4 and facilitates the transfer of parental histones to lagging strands
... to DNA replication in addition to nucleosome ...parental histones are inherited by daughter cells that retain “stemness,” whereas the differentiated cells contain mostly new histones (50, ...
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Zinc finger transcription factor CASZ1 interacts with histones, DNA repair proteins and recruits NuRD complex to regulate gene transcription
... complex, DNA repair protein, cofactors Received: March 16, 2015 Accepted: July 27, 2015 Published: August 07, 2015 ABSTRACT The zinc finger transcription factor CASZ1 has been found to control neural ...
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DNA looping, histones and DNA methylation are coupled by DNA oxidation to modulate transcription
... in DNA replication fidelity by preventing base substitutions or repeat sequence ...synthesized DNA strand that contains the mispaired ...MutH, DNA helicase II (MutU/UvrD), four exonucleases (ExoI, ...
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The central role of chromatin in autoimmune responses to histones and DNA in systemic lupus erythematosus
... subnucleosome complexes, three forms of chromatin, and DNA. Whole chromatin was the most reactive substrate, with 88% of the patients positive. By chi-square analysis, only the presence of anti-(H2A-H2B), ...
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Antibodies to Histones in Drug Induced and Idiopathic Lupus Erythematosus
... including histones, are removed but nuclear DNA is retained. Histones can be reconstituted back to nuclear DNA in acid-extracted tissue sections so that the resulting nuclear substrate is ...
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Histones and all that
... mutation that causes Huntington’s disease, the control of gene switching is thrown into chaos because of the mutant huntingtin protein. One defense mechanism cells have against gene switching problems is to coil the ...
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The cnidarian Hydractinia echinata employs canonical and highly adapted histones to pack its DNA
... Acknowledgements We wish to thank Prof. Gunter Plickert for help with RNA extractions and sample preparation, James Gahan for genomic DNA extractions, Dr. Andrew Cassin for help in database generation and ...
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Extracellular Histones Inhibit Efferocytosis
... with DNA (33). Whereas histones have also been demonstrated to be secreted and to con- tribute to mortality in sepsis through a role postulated to be associated with en- hanced endothelial cytotoxicity ...
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Epigenetic modifications of histones in cancer
... on histones for modification or through crosstalk through conferring a conformational change, thus alter- ing the downstream signaling and gene expression regu- ...as DNA damage and oxidative ...
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Posttranslational Modifications Occur In Histones
... Many domains are recognizable for a direct connection to reading and modifying the histone code. Fujiki R, Hashiba W, Sekine H, Yokoyama A, Chikanishi T, Ito S, Imai Y, Kim J, He HH, Igarashi K, Kanno J, Ohtake F, ...
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The sequential modifications of histones form the basis
... Knockdown of dLKR/SDH resulted in a dramatic shift in the kinetics of H3R17me2 methylation, with levels at a maximum between 4 – 7 min and decreased again from 10 min onwards ( Fig. 6 A ). This experiment was validated ...
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The effect of linker histones on force-induced nucleosome unstacking
... The flowcell preparation protocol using anti-digoxigenin and BSA was optimalized for DNA to yield the highest percentage of WLC-behaving force-extension curves. It was demonstrated that this protocol also works ...
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Changes in epigenetic marks on histones along melanoma genesis
... de DNA e modificações pós-traducionais em histonas são os mecanismos epigenéticos mais ...de DNA aberrante em melanomas, ainda são escassos os estudos sobre marcas em histonas nestes ...de DNA à ...
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Epigenetic marking of sperm by post-translational modification of histones and protamines
... containing DNA, histones and protamines was re-suspended in solubilization buffer containing 50 mM Tris-Cl pH ...salt-extracted histones and protamines were precipitated with trichloroacetic acid ...
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The role of extracellular histones in critical illness and their application in acute pancreatitis
... circulating histones 271 ...of histones into the circulation where they can be ...circulating histones with pancreatic necrosis scores in mouse models of acute pancreatitis supports the notion that ...
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Epigenetic marking of sperm by post-translational modification of histones and protamines
... On histones, PTMs are known to change protein structure and DNA binding by altering the electrostatic properties of ...of histones and decrease their affinity for the negatively charged DNA, ...
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Acetylation of intragenic histones on HPV16 correlates with enhanced HPV16 gene expression
... of DNA was diluted in ChIP buffer and antibodies indicated in the figures were added for an overnight end-over- end incubation at 4 ...the DNA on spin columns followed by DNA ...
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Quantitative profile of lysine methylation and acetylation of histones by LC-MS/MS
... 28 Chromatin structure and function are regulated by histone PTMs via structural modifications or by recruiting chromatin-associated factors. The first mechanism is a “direct structural perturbation”, as Bannister and ...
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A Class II Histone Deacetylase Acts on Newly Synthesized Histones in Tetrahymena
... THD2 is alternatively spliced. The THD2 coding sequence was determined by cloning and sequencing its cDNA (as de- scribed in Materials and Methods). Amplifying the cDNA with gene-specific primers revealed two cDNAs of ...
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