DNA extraction and amplification
Integrated DNA extraction and amplification on a microfluidic device
... system. DNA extraction and pre-concentration was performed ‘off-chip’ using commercial ...The DNA sample was then manually injected into a 160 nl PCR chamber along with all the necessary reagents to ...
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Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasmas in grapevine
... plant DNA extraction and 4 procedures for amplifica- tion in polymerase chain reaction of phytoplasma DNA were examined in parallel using the same plant tissues infected with ...The DNA ...
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Methods for microbial DNA extraction from soil for PCR amplification
... of DNA-based techniques can overcome this limitation by allowing the fate of particular genes or organisms to be monitored directly in environmental ...extract DNA from soil and sediment initially used ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... Finally, as we have already mentioned, the main reason for optimising the DNA extraction protocol was to analyse parasite infection in Lutzomyia spp. by PCR amplification. In this sense, it is ...
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Detection of Chlamydia trachomatis DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems
... Gel Extraction Kit ® + CTAB gave the highest sensitiv- ity to detect ...EB DNA from SF in combination with the ...EB DNA were achieved using alkaline lysis, QIAmp Tissue Kit ® and QIAmp Stool Kit ® ...
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Simple and quick method of DNA extraction from different parts of plant for PCR amplification
... the DNA molecular marker based genetic ...genomic DNA is the prerequisite for molecular biology research. An efficient DNA extraction method should be simple, rapid and affordable to provide ...
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A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction
... isothermal amplification assay for rapid detection of hepatitis B virus in human blood at 65 °C in less than 60 min and each test costs ...with DNA extraction revealed the ability to detect HBV ...
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Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... isothermal amplification (LAMP) method is a DNA amplification tool in which the DNA amplification can be achieved by incubation at a stable ...
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The detection of Atlantic cod (Gadus morhua) using loop mediated isothermal amplification in conjunction with a simplified DNA extraction process
... rapid extraction method described and a visual detection dye, enables sensitive molecular testing for ...morhua DNA whilst only requiring the facility for pipetting and a waterbath to provide the necessary ...
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Strategies for the Extraction, Purification and Amplification of Metagenomic DNA from Soil Growing Sugarcane
... microbial DNA in the environment require efficient extraction of the DNA from environmental samples and adequate purification from the co-extracted contaminants that inhibit PCR [27] ...[28]. ...
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Bringing Laboulbeniales into the 21st century: enhanced techniques for extraction and PCR amplification of DNA from minute ectoparasitic fungi
... for extraction of Laboulbeniales DNA involves using freshly collected material preferably stored in 95 % ...our DNA isolation positive ...good DNA preservation for a prolonged period of time. ...
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Evaluation-independent system for DNA section amplification
... The DNA detection process requires four steps: DNA extraction, DNA amplification, electrophoresis, and gel image analysis ...typical DNA detection method uses a gel documentation ...
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A Real-time Resistance Measurement for DNA Amplification and Detection
... of DNA-LAMP (Eiken Chemical, Japan). Gradient diluted DNA was used to test the sensitivity of the reaction ...the DNA extraction and LAMP reaction, to determine the anti-interference ability ...
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Evaluation of the illumigene Mycoplasma Direct DNA Amplification Assay
... continuous DNA amplification under isothermal ...additional DNA extraction step, making it faster and more economical than other NAAT assays that require the DNA extraction ...
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PREP-RAPID DNA Extraction Kit. PREP-RAPID Genetics DNA Extraction Kit USER MANUAL
... the extraction/preparation of amplification reactions and for the amplification/detection of amplification ...an amplification product in the area designed for ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... article bau061. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Bolchacova E, Voigt K, Crous PW, Miller AN, Wingfield MJ, Aime MC, An KD, Bai FY, Barreto RW, Begerow D, Bergeron MJ, Blackwell ...
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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
... the DNA of the specimens in the ...on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time ...
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Forensic DNA collection: extraction of molecular information from buccal cells using direct amplification
... 46 the data acquired from the wetted swabs which were rubbed 4 times in circular motions on two cheeks (2-C-4-Y) and wetted swabs that were rubbed across 2 cheeks, 4 times in up- down/side-to-side motions (2-U-4-Y). The ...
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Evaluation of DNA and RNA Extraction Methods
... smaller amplification products 16 . This suggests that the DNA extracted from FFPE samples are highly fragmented, and lower amplimer size products will have a higher success rate using ...PCR ...
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Extraction of DNA Extraction of DNA
... of DNA • The DNA can be stained by the inclusion of ethidium bromide in the gel, or by soaking the gel in a solution of ethidium bromide after ...The DNA shows up as an orange band on illumination by ...
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