DNA extraction from environmental and faecal samples
Comparison of five assays for DNA extraction from bacterial cells in human faecal samples
... extracted from microbes in faecal samples is of utmost importance to optimize the information that can be obtained from these molecular ...different extraction methods may have ...
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Detection of Clostridium botulinum group III in environmental samples from farms by real time PCR using four commercial DNA extraction kits
... compare DNA extraction procedures, but most of them focus only on one matrix or one pathogen, and their efficiency in extracting DNA varied among these ...parameters. Extraction methods ...
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
... 13 Gels were made with ~125 ml of 1% agarose stained with 2 μl of Ethidium bromide. All of the PCR reaction was loaded into gel wells with 4 μl of loading dye. Gel electrophoresis was conducted at 100 Volts and 400 mAmps ...
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A semi automated magnetic capture probe based DNA extraction and real time PCR method applied in the Swedish surveillance of Echinococcus multilocularis in red fox (Vulpes vulpes) faecal samples
... These samples were spiked with 1–13 eggs derived from a gravid ...isolated from one of the positive foxes in the Swedish monitoring during ...the extraction tubes and this was the reason for ...
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Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels
... and Environmental Engineering, Stanford University, Stanford, CA, United States, 4 Monterey Bay Aquarium Research Institute, Moss Landing, CA, United States Metabarcoding of marine environmental DNA ...
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Environmental DNA from seawater samples correlate with trawl catches of subarctic, deepwater fishes
... Influences from other factors It is obvious that an initial relationship between fish density and eDNA sequence abundance can be obscured by several sources of ...obtained from DNA extraction, ...
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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
... two DNA extraction methods, several annealing temperatures for nested PCR reactions targeting the 18S, SSU rRNA (small subunit ribosomal RNA), and the GP60 (60 kDa glycoprotein) genes, and two types of ...
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Are Mussel Shells Environmental DNA Time Capsules? A Comparison Of Extraction Methods For Obtaining DNA From Shell Material.
... Tissue DNA Extraction This DNA extraction kit was used for the isolation of genomic DNA from hinge ligament tissue by using a spin column binding in combination with a specific ...
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PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes
... DNA extraction technique Although the present addition of flotation fluids to frozen faecal samples, followed by sieving, may deform frozen larvae and prevent morphological identification ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... article bau061. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Bolchacova E, Voigt K, Crous PW, Miller AN, Wingfield MJ, Aime MC, An KD, Bai FY, Barreto RW, Begerow D, Bergeron MJ, Blackwell ...
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Extraction of DNA from Difficult Samples with an Automatable Portable System
... [5]. DNA extracted from soil allows conclusions on the composition of the microflora and the purification and analysis of DNA from specific ...for extraction of DNA from ...
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Comparison of Different DNA Extraction Methods for Forensic Samples
... Abstract DNA analyses are important for different types of research in biomedical and forensic science and DNA isolation is an often required ...different DNA isolation kits are constantly becoming ...
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Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction
... bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and ...
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Design and application of supramolecular solvents and adsorbents to the extraction of contaminants from food and environmental samples
... their extraction with coacervates made up of decanoic acid reverse micelles and subsequent determination by liquid ...the extraction of ...selectivity. Extraction efficiencies and concentration ...
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Evaluation and statistical analysis of the modified QuEChERS method for the extraction of pinoxaden from environmental and agricultural samples
... collected from a pilot field in ...grain samples. The pro- posed method is a milestone in the extraction of pinoxa- den, a relatively new pesticide, from agricultural and environmental ...
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GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS
... blood samples collection is the family trio, ...blood samples from about 20 trios (60 individuals) from each geographic location under ...
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Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients
... amplify DNA in crude prepared urine samples ...amplify samples extracted with the Mu DNA GenoLyse ...(4) samples became negative for qPCR but positive for Mu RPA, resulting in a reduced ...
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A review on isolation of dna from botanical samples and their dna fingerprinting
... isolate DNA from Sorghum (Sorghum bicolour ...µl extraction buffer was added to each ...The DNA pellet was re-suspended in 100µl high salt TE with RNase A and incubated at 60 o C for 30 ...TE ...
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Prevalence of antibiotic resistance genes in faecal samples from cattle, pigs and poultry
... isolated from the faeces of cattle, pigs or ...the DNA purified from the faeces of farm ...the DNA purified from the faeces of cattle, pigs and laying ...pig faecal ...
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Metagenomics detection and characterisation of viruses in faecal samples from Australian wild birds
... biological samples by the same research group 11–13 ...biological samples with known viruses, which would have served as a useful positive control to validate the ...in faecal samples ...
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