DNA helicase activity
Phosphorylation of MCM4 at Sites Inactivating DNA Helicase Activity of the MCM4-MCM6-MCM7 Complex during Epstein-Barr Virus Productive Replication
... conserved DNA-dependent ATPase motifs in the cen- tral domain (3, 44) and form several stable subassemblies, including MCM2-3-4-5-6-7, MCM2-4-6-7, MCM4-6-7, and MCM3-5 complexes (35, 49, ...56). DNA ...
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Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... RNA helicase activity, not the DNA helicase activity, in W501 mutants was ...ATPase activity or substrate binding activ- ity in the mutant enzymes was responsible for the ...
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Rep-Mediated Nicking of the Adeno-Associated Virus Origin Requires Two Biochemical Activities, DNA Helicase Activity and Transesterification
... of DNA replication also contained such improbable structures that appear necessary for in vivo origin ...circular DNA superhelix to melt the loop region and pro- mote cruciform extrusion ...Rep DNA ...
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... the DNA helicase reaction, we noted that replacement of ribose with deoxyribose significantly increased the effectiveness of the ...of DNA helicase ...
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DNA Helicase Activity Is Associated with the Replication Initiator Protein Rep of Tomato Yellow Leaf Curl Geminivirus
... Rep122-359 activity, resembling the require- ments of other helicases, among them MCM, where increasing the length of the 5 ⬘ tail as well as that of the 3 ⬘ tail enhances processivity ...replicative ...
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Biochemical Characterization of Adeno-Associated Virus Rep68 DNA Helicase and ATPase Activities
... endonuclease activity, provided that the sequence in the im- mediate vicinity of the trs is ...Rep helicase to unwind the trs site prior to nicking or a need to use the translocation machinery of the Rep ...
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The Main Role of Srs2 in DNA Repair Depends on Its Helicase Activity, Rather than on Its Interactions with PCNA or Rad51
... topoisomerase activity on misincorporated ...Top1 activity and promote resection by enhancing Exo1 ...the DNA helicase domain alone is sufficient to deal with various types of DNA ...
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Bipartite Structure of the SGS1 DNA Helicase in Saccharomyces cerevisiae
... that helicase activity is also re- ⌬N322, ⌬N644, and ⌬C795 levels were greatly ...basic helicase domain alone, mi- noticeably ...requires DNA helicase activity and all but the ...
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The Papillomavirus E1 Protein Forms a DNA-Dependent Hexameric Complex with ATPase and DNA Helicase Activities
... has DNA helicase ...a DNA helicase assay, we analyzed the material from the gradient peak in an oligonucleotide displace- ment assay, as described by Seo and Hurwitz ...
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Characterization of Plasmodium falciparum ATP dependent DNA helicase RuvB3
... higher helicase activity than PfRuvB2 alone ...stronger DNA helicase activity than the individual pro- teins ...ATPase activity but lacks helicase function ...appreciable ...
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Pestivirus NS3 (p80) protein possesses RNA helicase activity.
... RNA helicase activities all reside within the same polypeptide chain (2, 8–10, ...proteinase activity has been ex- perimentally demonstrated (1, 3, 11, 12, 26, 36, ...NTPase activity (33, 34, 37, ...
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DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase.
... M13 DNA substrate (Figure 2, lanes 6 – ...on DNA replication (Figure 2 lanes 1 – ...prevents DNA replica- tion. Cascade R-loop formation most likely blocks DNA repli- cation elongation at ...
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Molecular insights into DNA interference by CRISPR associated nuclease helicase Cas3
... Proposed DNA interference mechanism by Cas3 in association with Cascade effector ...complementary DNA strand of invading dsDNA, resulting in displacement of the noncomplementary DNA ...displaced ...
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DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase
... spacer DNA into CRISPR ...MGE DNA fragments and their subsequent integration into a CRISPR ...duplex DNA molecule that posi- tions DNA 3´ OH groups appropriately for integration into CRISPR as ...
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DNA substrate recognition and processing by the full-length human UPF1 helicase
... RNA helicase, where the enzyme engages with a 5 single- stranded NA tail and translocates upon it during strand displacement (19), we used a partially single- and double- stranded DNA test substrate with a ...
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Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1
... the helicase activity of R505E is surprising since it is impaired in all activities, including ssDNA binding, required for ...T7 helicase gp4A 0 (23), require a substrate with an ssDNA tail that is ...
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AcademH, a lineage of Academ DNA transposons encoding helicase found in animals and fungi
... domain was predicted between β5 and α4, as are the cases of RAG1 and Hermes. Another insertion domain was pre- dicted between β2 and β3, unlike any other transposases. Many of Academ families encode a large protein that ...
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Requirement for the E1 Helicase C-Terminal Domain in Papillomavirus DNA Replication In Vivo
... assembled helicase; in this model, a negatively charged region of the FB/CTM from each monomer was proposed to contact a positively charged cleft on the adjacent monomer so that each CTD would act as an ...
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Interaction with the Epstein-Barr Virus Helicase Targets Zta to DNA Replication Compartments
... smaller DNA viruses, herpesviruses encode many of the enzymes necessary for DNA replication, and it may be advantageous for these viruses to replicate their genomes at a time when they are not competing ...
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The Autographa californica Nuclear Polyhedrosis Virus p143 Gene Encodes a DNA Helicase
... Nuclear extracts were prepared from Sf9 cells 48 h after infection with vAc-P143. Analysis of total cell proteins by SDS- PAGE showed overexpression of a protein with the expected size of P143 (Fig. 1, compare lanes 2 ...
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