DNA I
Cessation of reentry of simian virus 40 DNA into replication and its simultaneous appearance in nucleoprotein complexes of the maturation pathway.
... To determine the reentry kinetics for newly synthesized DNA during the BUdR chase, DNA was extracted by the Hirt method, form I DNA was isolated on alkaline sucrose gradients, and HL DNA[r] ...
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Replication of parvoviral DNA. I. Characterization of a nuclear lysate system.
... Nuclear lysates containing unlabeled DNA or DNA labeled for 1 h with [3H]TdR before cell harvest were prepared and incubated as described in the text, except that dNTP's were omitted fro[r] ...
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RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses I. Directing Influence of DNA in the Reaction
... Intfluence of Neurospora nuclease priming activity of DNA templatesa on dTMP incor- Expt Additions poration pmoles/ 30 min 1 Complete system deoxyribonuclease I-treated Complete system +[r] ...
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Topoisomerase I-mediated integration of hepadnavirus DNA in vitro.
... Lanes: 1 and 10, Topo I treatment of WHV plus C3 DNA mixtures followed by PCR using C3 primer e and WHV minus-strand primer b; 2 and 11, control reactions for lanes 1 and 10 in which Top[r] ...
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Temperature and Magnetic Field Driven Modifications in the I V Features of Gold DNA Gold Structure I V
... connectivity, I-V characteristics were measured in presence of magnetic ...the I-V curve for connection of Au-gap-Au at the absence of ...without DNA, which produced a current of ~10 −11 ...placing ...
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Quantitation of a simian virus 40 nonhomologous recombination pathway.
... Using this assay to measure the efficiency of recombination with SV40 DNA in permissive monkey BSC-1 cells, we found that: i over a range of DNA concentrations, polyomavirus DNA which is[r] ...
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Mutations that specifically impair the DNA binding activity of the herpes simplex virus protein UL42.
... the DNA binding activity of UL42 appears to be required for HSV DNA replication, one approach to the design of an anti-HSV drug could be the interruption of the UL42-DNA ...the DNA-UL42 in- ...
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Voltammetric Study of Sudan I on Glass Carbon Electrode and Its Interaction with DNA
... between DNA and molecules [14-17]. Sudan I is a kind of carcinogen, it will do harm to human health when it enters human ...Sudan I and DNA is helpful to understand the pathogenesis, which is ...
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Protein synthesized early after infection is linked to the termini of adenovirus type 2 DNA synthesized in vivo and in vitro.
... Three lines of evidence are presented to show that viral 3H-labeled DNA prepared by this procedure was linked to CBP: i the DNA sedimented more rapidly than protein-free DNA i.e., protei[r] ...
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Mutations in the Human Immunodeficiency Virus Type 1 Integrase D,D(35)E Motif Do Not Eliminate Provirus Formation
... viral DNA in cells infected with virus encoding mutations at each of the three residues of the D,D(35)E ...viral DNA to be colinear with the incoming viral genome. DNA sequencing of the junctions ...
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Review Paper on Feasibility Study of Solar Energy for Brt’s Road
... calculations. DNA has cutting, copying, pasting, repairing and many other ...one DNA at a time, rather, many copies of the enzyme can work on many DNA molecules ...Therefore, DNA computing is ...
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A novel mutation in DNA topoisomerase I of yeast causes DNA damage and RAD9-dependent cell cycle arrest.
... Extracts from a strain express- ing wild-type TOPI (NLY 107) fully convert the super- coiled substrate plasmid to the relaxed form, whereas extracts from the isogenic topl[r] ...
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Anatomy of Herpes Simplex Virus DNA. VIII. Properties of the Replicating DNA
... iii Electron microscopic studies indicated that the DNA extracted from cells replicating viral DNA and banding at the density of viral DNA contained: a linear, full-size molecules with i[r] ...
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Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.
... Biological function of TRF and TOPl gene prod- ucts: TRFl (HPRl), TRF2 and TRF4 do not encode pro- teins that are essential for proper top0 I1 transcription, translatio[r] ...
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MUTAGENESIS IN OOCYTES OF DROSOPHILA MELANOGASTER. I. SCHEDULED SYNTHESIS OF NUCLEAR AND MITOCHONDRIAL DNA AND UNSCHEDULED DNA SYNTHESIS
... When females where homozygous for a mutant allele at the mei- 9 locus that was excision-repair deficient in tissue culture (BOYD, GOLINO and SETLOW 1976), an increase in th[r] ...
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Mechanism of Degradation of Duplex DNA by the DNase Induced by Herpes Simplex Virus
... HSV-I specific DNA polymerase activity 0 and the release of acid-soluble nucleotides A were measured for calf thymus DNA which had been treated for the indicated times with A DNase I, B [r] ...
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Preservation effects of geniposidic acid on human keratinocytes (HaCaT) against UVB
... acid, I used DPPH radical scavenging activity. As the re- sult of analysis, I found that when I treated it with geni- posidic acid of each density of 10, 20, 30 and 40 μM, its anti-oxidation effect ...
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Avian Retrovirus pp32 DNA-Binding Protein I. Recognition of Specific Sequences on Retrovirus DNA Terminal Repeats
... Binding of pp32 to supercoiled pPvuII-DG DNA followed by digestion of this complex with a multicut restriction enzyme 28 fragments total permitted pp32 to preferentially retain on nitroc[r] ...
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Transfection of Escherichia coli Spheroplasts IV. Transfection of rec+ and rec− Spheroplasts by Native, Denatured, and Renatured T5 Bacteriophage DNA After Repair of Single-Strand Breaks by Polynucleotide Ligase
... One possible explanation for the two-hit nature of the plot of infective centers versus DNA concentration is the extreme shear sensitivity of T5 phage DNA 1, 13; if the DNA were broken i[r] ...
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Acquisition of New DNA Sequences After Infection of Chicken Cells with Avian Myeloblastosis Virus
... Therefore, we have used DNA-RNA hybridization in DNA excess to investigate the nature of the DNA sequences complementary to AMV, RNA present in i gs antigen-positive, non-virus-producing[r] ...
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