DNA integration
The structure of adenovirus type 12 DNA integration sites in the hamster cell genome.
... Foreign DNA can integrate into the genomes of mammalian cells, and this process plays major roles in viral oncogenesis and in the generation of transgenic organisms and will be important in evolving regimens for ...
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Relationship between Retroviral DNA Integration and Gene Expression
... their DNA into a large number of sites in the host genome, factors controlling the specificity of integration remain controversial and poorly ...on integration in vivo, we created quail cell clones ...
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A supramolecular assembly mediates lentiviral DNA integration
... target DNA bending ...target DNA side of the complex highlighted several patches of positive charge, each corresponding to the cleft at the IN CCD dimerization interface (6) ...nucleosomal DNA in the ...
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Repair of Gaps in Retroviral DNA Integration Intermediates
... gapped integration intermediate in vivo because this protein is ubiquitously expressed and known to be involved in base excision ...low DNA concentrations such as those used here are not sufficient for end ...
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A supramolecular assembly mediates lentiviral DNA integration
... (U5+U3). DNA migrating in agarose gels as FS products was cloned and sequenced to establish precise insertion positions and identities (U3 or U5) of the vDNA end(s) in each ...target DNA, as well as ...
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Selective sites of adenovirus (foreign) DNA integration into the hamster genome: changes in integration patterns.
... The experimental conditions that can lead to changes in the Adl2 integration patterns, as evidenced by the approach to similar off-size band patterns generated with five different restri[r] ...
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AN IN SILICO APPROACH TO IDENTIFY NEW ANTI HIV INTEGRASE INHIBITOR LIKE LEADS BY DOCKING STUDIES
... the integration between viral DNA to host DNA Strand, this process important for virus replication cycle 4 , because human itself no such type of enzymes, so inhibition mechanism is selective without ...
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Gammaretroviral Integration into Nucleosomal Target DNA In Vivo
... retroviral integration targeting reported that sites of gammaretroviral DNA integration were positively correlated with DNase I-hypersensitive sites in ...gammaretroviral integration sites and ...
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Construction and Characterization of an in-vivo Linear Covalently Closed DNA Vector Production System
... lcc DNA integration, we employed an in vivo approach by exploiting λ and Φ80 Int-att site-specific recombin- ation systems [25] in a Rec + ...assess integration efficiency following transformation of ...
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Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae
... complex, integration had occurred by HR, which was comparable with the wild-type (Table ...after integration by HR (44 versus 90%, ...of integration by HR is not altered in cells lacking a functional ...
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Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus.
... Although restriction enzyme analysis of cell-virus DNA junction fragments indicated that these viral DNA sequences are located in at least 29 distinct integration sites, many strains car[r] ...
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Directed integration of minute virus of mice DNA into episomes.
... of DNA synthesis; thick gray lines, parental hairpin palindromic sequences at the viral termini; thick black lines, newly synthesized palindromic DNA; thin gray lines, parental strand; thin black lines, ...
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The integration of adenovirus DNA during productive and abortive infection
... Other manifestations of the expression of endogenous viral genes are the presence of virus-specific RNA in apparently uninfected cells lacking the group specific antigen Hayward and Hana[r] ...
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Efficient autointegration of avian retrovirus DNA in vitro.
... For gel analysis of the in vitro integration reaction product, 1 ,ug of 4XX174 RESULTS For studying integration of ALV DNA in vitro, we reasoned initially that viral DNA in acutely infec[r] ...
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Identification of a CpG Island Methylator Phenotype that Defines a Distinct Subgroup of Glioma.pdf
... (A) Integration of the samples within each DNA methylation and gene expression ...each DNA methylation cluster (red, cluster 1 [G-CIMP]; blue, cluster 2; green, cluster 3) are indicated for each gene ...
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DNA Palindromes with a Modest Arm Length of ≳20 Base Pairs Are a Significant Target for Recombinant Adeno-Associated Virus Vector Integration in the Liver, Muscles, and Heart in Mice
... rAAV integration at DNA palindromes was not revealed in our previous study, in which we investigated rAAV integrations in mouse liver using a he- reditary tyrosinemia type I mouse model and in vivo ...
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Intracellular Forms of Adenovirus DNA III. Integration of the DNA of Adenovirus Type 2 into Host DNA in Productively Infected Cells
... When an alkaline sucrose density gradient is "overloaded" by lysing 200,000 cells in alkali in an SW41 tube instead of the usual 30,000 cells lysed in a "regular" experiment, the same se[r] ...
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Mechanism of transfection of chicken embryo fibroblasts by Rous sarcoma virus DNA.
... genetically RecipFoci/ Colonies/ Stable transformation of resistant cells 8 days DNA ient plate" plate' after transfection could be due either to direct integration of donor RSV DNA or t[r] ...
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cis-active elements from mouse chromosomal DNA suppress simian virus 40 DNA replication.
... However, we demonstrate that mouse DNA sequences present in the SV40 late region of the rescued clone and derived from sequences flanking the SV40 integration site in VLM DNA suppress re[r] ...
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Topoisomerase I-mediated integration of hepadnavirus DNA in vitro.
... Lanes: 1 and 10, Topo I treatment of WHV plus C3 DNA mixtures followed by PCR using C3 primer e and WHV minus-strand primer b; 2 and 11, control reactions for lanes 1 and 10 in which Top[r] ...
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