DNA packaging
A director field theory of DNA packaging in bacteriophage viruses
... by DNA as it is packed within ...packaged DNA posit spool-like geometries (Earnshaw and Har- rison, 1977), toroids (Hud, 1995) and other geometries (Black et ...duplex DNA genome of the T4 ...
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An RNA Domain Imparts Specificity and Selectivity to a Viral DNA Packaging Motor
... of packaging, we sought to deter- mine the effect of the pRNA copy number on this ...phage packaging motors operate as rings, the question of whether a full complement of domain II in the motor is necessary ...
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Herpes Simplex Virus DNA Packaging without Measurable DNA Synthesis
... 1 DNA synthesis and packaging occur within the nuclei of infected cells; however, the extent to which the two processes are coupled remains ...Correct packaging is thought to be dependent upon ...
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Adenovirus with DNA Packaging Gene Mutations Increased Virus Release
... The goal of this study was to investigate which genes in Ad genome may affect Ad oncolytic efficacy, and, therefore, may be modified and combined with other approaches in the development of future oncolytic Ads. The Ad ...
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Structure-Based Mutagenesis of Sulfolobus Turreted Icosahedral Virus B204 Reveals Essential Residues in the Virion-Associated DNA-Packaging ATPase
... Putative roles for pseudoconserved residues within the Walker A motif. In the crystal structure of B204 complexed with AMP-PNP, the side chains of Lys13 and Arg14 are not in close proximity to the nucleotide or any other ...
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Defining cosQ, the Site Required for Termination of Bacteriophage λ DNA Packaging
... OLLOWING DNA replication, many large, double- a concatemer to initiate ...stranded DNA viruses, such as the herpes and pox bacteriophage is composed of three subsites: cosN, viruses and bacteriophage , ...
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Stability and in vitro DNA packaging of bacteriophages: effects of dextrans, sugars, and polyols.
... DISCUSSION Dextrans were found to: i protect bacteriophages from osmotic shock, ii increase the efficiency of the in vitro assembly of infective bacteriophage T7 from DNA-free procapsids[r] ...
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Semiflexible polymers: fundamental theory and applications in DNA packaging
... the packaging motor of φ29 suggest the potential for twist to play a role in the packaging ...φ29 packaging motor, a mechanism for DNA translocation has been proposed whereby either the motor ...
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DNA packaging by the Bacillus subtilis defective bacteriophage PBSX.
... The genetic data suggest that DNA from the chromosomal region of prophage PBSX is packaged less frequently and not in direct proportion to its abundance at the time of induction than DNA[r] ...
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DNA packaging in vitro by an isolated bacteriophage T7 procapsid.
... It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts de[r] ...
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Bacteriophage P22 in vitro DNA packaging monitored by agarose gel electrophoresis: rate of DNA entry into capsids.
... Therefore, in the present study, the P22 procapsid and mature bacteriophage were electrophoretically characterized, procedures for increasing the efficiency of in vitro packaging were de[r] ...
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Model for DNA packaging into bacteriophage T4 heads.
... When bacteriophage T4 cs2O-ts21-t empty heads which accumulate in vivo at low temperature are converted into mature phage by raising the temperature ts2l prevents maturation of preheads [r] ...
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Topoisomerase II and other DNA-delay and DNA-arrest mutations impair bacteriophage T4 DNA packaging in vivo and in vitro.
... Temperature shift and in vitro packaging experiments with the am39 mutant indicated that packaging of normal DNA occurs in the absence of topoisomerase II function, yet in vivo and in vi[r] ...
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Herpes Simplex Virus 1 Small Capsomere-Interacting Protein VP26 Regulates Nucleocapsid Maturation
... impaired packaging of replicated viral DNA genomes into capsids but had no effect on viral DNA concatemer ...viral DNA but not scaffolding proteins, and produced an accumulation of type A ...
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Identification, Subviral Localization, and Functional Characterization of the Pseudorabies Virus UL17 Protein
... on DNA-containing C-capsids ...viral DNA (as demonstrated by its electron-dense interior), gold particles were concentrated in the center of the ...replicated DNA into the capsid and suggest that the ...
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Constructing Empirical Likelihood Confidence Intervals for Medical Cost Data with Censored Observations
... stranded DNA bacteriophages is highly packed in an outer shell of ...mature DNA, the mechanism to generate the DNA is ...this DNA include: i) the mechanism for the end-formation of the ...
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The Gene Product of Human Cytomegalovirus Open Reading Frame UL56 Binds the pac Motif and Has Specific Nuclease Activity
... clude that mutation of the HSV-1 ICP 18.5 protein and the PrV ICP 18.5 homolog exhibit a viral DNA packaging defect. Recombinant ICP 18.5 protein from baculovirus-infected cell extracts and possibly from ...
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Role for the Adenovirus IVa2 Protein in Packaging of Viral DNA
... the packaging sequence and have deletions of all the viral protein coding genes (7, 9, 36), result- ing in improved duration of expression of therapeutic genes in vivo (14, 35, 36, 42, 50, 51, 59, ...vector ...
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Optimal lengths for DNAs encapsidated by Epstein-Barr virus.
... We have observed that i the efficiency of DNA packaging by EBV is dependent on the length of the DNA being packaged, such that a specific range of DNA lengths is packaged more efficientl[r] ...
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On the sequential packaging of bacteriophage P22 DNA.
... These results support the notion that the protein products of gene 2 and gene 3 are both more stringently required for initiation of sequential DNA packaging series than for their extens[r] ...
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