DNA Polymerase ɛ and the S Phase Checkpoint
Exploring the roles of the C terminal of Pol2, the catalytic subunit of DNA Polymerase ɛ, in replication and checkpoint response
... delayed checkpoint activation observed in pol2-11 cells compared to the wild type, I hypothesised that expression of the C- terminus could somewhat suppress this phenotype, as it had been shown to improve the ...
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Regulation of DNA replication by the S-phase DNA damage checkpoint
... to DNA damage. This slowing was the first DNA damage checkpoint response discovered and its study led to the discovery of the central checkpoint kinase, Ataxia Telangiectasia Mutated ...the ...
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The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε
... Copyright and reuse: The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the ...
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The Schizosaccharomyces pombe S-Phase Checkpoint Differentiates Between Different Types of DNA Damage
... UV-induced S-phase delay, there remains a small but reproducible difference in the level of FACS signal at the later timepoints in Figure ...or S-phase (Figures 1A, 3A, ...subtle ...
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SIRT1 Deacetylates TopBP1 and Modulates Intra-S-Phase Checkpoint and DNA Replication Origin Firing
... intra-S-phase checkpoint, and chromosome ...in DNA replication fork protection and replication origin ...modulating DNA replication fork initiation and the intra-S-phase ...
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DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication
... for DNA-PK in the effi cient destabilization of histone mRNA during replication ...and DNA-PK ...and DNA-PK may not al- ways be fully redundant with each other for histone mRNA ...
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“DNA Binding Region” of BRCA1 Affects Genetic Stability through modulating the Intra-S-Phase Checkpoint
... essential DNA binding region (DBR: 421-701 amino acids) within the central region of human BRCA1, and found that BRCA1 brings DNA together and preferably binds to splayed-arm DNA in a ...
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The Fission Yeast Rad32(Mre11)–Rad50–Nbs1 Complex Acts Both Upstream and Downstream of Checkpoint Signaling in the S-Phase DNA Damage Checkpoint
... MMS-induced checkpoint signaling is independent of Tel1: As an independent test of whether Tel1 contributes to Cds1 signaling, and if MRN is involved in activation of Cds1 in a Tel1-dependent manner in response to ...
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Mcl1p Is a Polymerase α Replication Accessory Factor Important for S-Phase DNA Damage Survival
... synchronous DNA replication in the presence of HU or MMS or exposure to UV ...on DNA replication and viability in the absence of treatment in the cdc10-129 back- ...increased DNA fluorescence in flow ...
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Drug-Sensitive DNA Polymerase δ Reveals a Role for Mismatch Repair in Checkpoint Activation in Yeast
... the DNA damage S-phase checkpoint response in Saccharomyces cerevisiae to slow lagging-strand DNA replication by exposing cells expressing a drug-sensitive DNA polymerase ...
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The Saccharomyces cerevisiae F-Box Protein Dia2 Is a Mediator of S-Phase Checkpoint Recovery from DNA Damage
... by checkpoint pathways that pause the cell cycle when stress arises to threaten the integrity of the ...of checkpoint pathways has been extensively studied, our understanding of how cells resume the cell ...
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SCF FBXW7α modulates the intra-S-phase DNA-damage checkpoint by regulating Polo like kinase-1 stability
... through S phase under normal conditions and in response to replication ...When DNA lesions are detected, replication fork progression is blocked allowing time for repair to avoid genomic instability ...
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PfuTurbo DNA Polymerase
... PfuTurbo DNA polymerase is a mixture of cloned Pfu DNA polymerase and the exclusive thermostable ArchaeMaxx polymerase-enhancing factor*** that enhances PCR product yields and increases ...
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DNA polymerase α (swi7) and the flap endonuclease fen1 (rad2) act together in the s phase alkylation damage response in S pombe
... Abstract Polymerase a is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand ...pombe polymerase a named swi7-1, abolishes imprinting required for mating-type ...the ...
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The Duck Hepatitis B Virus Polymerase Is Activated by Its RNA Packaging Signal, ɛ
... the polymerase is proposed to induce a physical alteration to the polymerase that is needed for it to develop enzymatic ...unusual DNA polymerase activity employing exogenous RNAs (the trans ...
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Taq2000 DNA Polymerase, Part Number Taq2000 DNA Polymerase, Part Number X Taq Polymerase Buffer
... Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Taq2000 DNA Polymerase Stop leak if without risk. Move containers from spill area. Dilute ...
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Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing
... intra-S phase checkpoint might influence timing if replication forks assembled early in S phase generated an inhibitory signal to prevent the initiation at later-firing ...normal ...
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The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence
... This shows that the decision for cell arrest was abolished and probably the nucleotide levels were sufficiently ad- equate to allow cell cycle restoration. Interestingly, the ordinary dynamics of the cell cycle is not ...
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PrimeSTAR HS DNA Polymerase
... HS DNA Polymerase provides high fidelity, high sensitivity, and high specificity amplification for applications such as DNA amplification from cDNA ...
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CiteSeerX — DNA Polymerase Profiling
... DNA Polymerase Profiling Daniel Summerer Abstract We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high ...the DNA polymerase ...
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