DNA probe
A non-spectroscopic optical biosensor for the detection of pathogenic Salmonella Typhimurium based on a stem-loop DNA probe and retro-reflective signaling
... A biotinylated stem-loop DNA probe, a target gene DNA, and mismatched target genes were synthesized by Bioneer (Daejeon, Korea). The retroreflective Janus particle-quantifying chips (RQC) were ...
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Comparison of Gen Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens
... True positive samples were identified in this study as those specimens that were positive by culture or positive by two nonculture tests i.e., DNA probe test and probe competition assay [r] ...
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Development of a DNA probe to detect Salmonella typhi
... Using various enteric bacterial strains, we tested probe specificity with nitrocellulose and Whatman 541 paper and found Whatman 541 paper to have several advantages when used in the col[r] ...
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Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe
... In summary, we found that for a well-defined subset of hospitalized patients colonized by Candida species before development of nosocomial candidemia, the colonizing and infecting strain[r] ...
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DNA probe for strain typing of Cryptococcus neoformans
... The DNA fingerprints were generated by hybridization of labelled UT-4p plasmid DNA to Accl-digested genomic DNA from the recovered isolates.. epidemiology and virulence of this pathogeni[r] ...
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DNA probe specific for Legionella pneumophila
... When unlabeled DNA from strain ATCC 33152 was cleaved by endonuclease BamHI, the DNA fragments cross-hybridizing with the labeled DNA from all of the other species and genera tested or w[r] ...
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Virulence factors of verotoxin producing Escherichia coli O157:H7
... A DNA probe to identify enterohem orrhagic Escherichia coli o f 0157:H 7 and other serotypes that cause hem orrhagic colitis and hem olytic uremic syndrome... Use o f DNA probe and HEp-2[r] ...
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Diagnosis of Sugarcane White Leaf Disease Using the Highly Sensitive DNA Based Voltammetric Electrochemical Determination
... The DNA based electrochemical strategies in particular the voltammetric analyses have been used as a critical tool for a discriminative study of nucleic acid conformation and modification altogether with the ...
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A Rapid, Label-free and Impedimetric DNA Sensor Based on PNA-modified Nanoporous Gold Electrode
... for DNA sensing [11]. PNA probe possesses two main advantages over DNA probe for hybridization: firstly, no electrostatic repulsion between PNA and negatively charged DNA, which make ...
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Ultrasensitive Biosensor for the Detection of Vibrio cholerae DNA with Polystyrene co acrylic Acid Composite Nanospheres
... target DNA was tagged with a universal fluorescein to allow DNA hybridisation recognition achieved via enzymatic conver- sion of α-naphthyl phosphate to electroactive α- ...for DNA hybridisation, ...
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Performance Assessment of New Multiplex Probe Assay for Identification of Mycobacteria
... The quest for stable features on which to base classifications and identifications has led to the development of techniques for analyzing lipids present in the mycobacterial cell wall (4) or genomic regions with ...
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A Comparison of Diagnostic Methods in Adolescent Girls With and Without Symptoms of Chlamydia Urogenital Infection
... The diagnostic methods used in this study were cell culture with monodonal antibody confirmation, a chemiluminescent DNA probe (Gen-Probe PACE 2, Gen-Probe Inc. San Diego CA),.. and two [r] ...
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Development and application of a microarray meter tool to optimize microarray experiments
... reduced probe con- ...higher DNA concentration performed sub-optimally. Elevated DNA probe concentrations coupled with this robot have a detrimental effect on experimental conditions possibly ...
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A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex.
... (a) FeBABE-DNA cleavage assay in PICs using nuclear extract containing Rad25-Flag. Rad25 cleavage fragments were visualized by Western blotting with anti-Flag antibody. The arrow points to full length Rad25 and ...
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Titration of integrated simian virus 40 DNA sequences, using highly radioactive, single-stranded DNA probes.
... In early attempts at hybridization in probe excess, asymmetric SV40 complementary RNA cRNA was used as a probe to titrate viral DNA sequences within transformed cell DNAs immobilized on [r] ...
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Development of a DNA hybridisation method for the identification of Rhizobium and Bradyrhizobium: a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in microbiology at Massey University, New Zealand
... PAGE The effect of the concentration of probe DNA in the hybridisation buffer on the intensity of colour produced on genomic DNA slot-blots hybridised with a homologous biotin-labelled D[r] ...
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Development of a DNA hybridisation method for the identification of Rhizobium and Bradyrhizobium: a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in microbiology at Massey University, New Zealand
... PAGE The effect of the concentration of probe DNA in the hybridisation buffer on the intensity of colour produced on genomic DNA slot-blots hybridised with a homologous biotin-labelled D[r] ...
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Herpes simplex virus type 1 origin-specific binding protein: oriS-binding properties and effects of cellular proteins.
... Incubation of infected cell extracts with the lower-affinity site II DNA as a probe resulted in the appearance of two protein-DNA complexes with mobilities identical to those of the A an[r] ...
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Genetic relatedness of equine herpesvirus types 1 and 3.
... Thus, the addition of the unlabeled second viral DNA EHV-3 to the hybridization reaction increased the concentration of sequences homologous to only a restricted portion of the probe DNA[r] ...
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Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus
... Because the number of marker genes is limited, estab- lishing a marker-recycling technique is necessary for the consecutive insertion of gene expression cassettes or multiple-gene deletions. For marker rescue, the ...
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