DNA-recognition
One-to-one motif for DNA recognition by beta-alanine-linked polyamides
... binding modes, a reversal of specificity effectively 82,000-fold compared to 2. The exceptional specificity of 19 may be attributed to the limited mobility of the bulky acetamide group at the turn position, which may in ...
178
Structural Basis for DNA Recognition by the Two Component Response Regulator RcsB
... for DNA recognition, we first determined requirements for RcsB-DNA complex formation in vitro and then determined its cocrystal ...bound DNA in the absence of a phosphoryl donor, but the ...
9
Structural Basis of DNA Recognition by Synthetic Ligands
... The DNA double helix presents functional groups in the major and minor grooves that can be used by ligands for readout of the base pair sequence.. The overall flexibility and shape also [r] ...
160
Genetic analysis of the DNA recognition sequence of the P2 Cox protein.
... To study the in vivo effects of the Cox protein on the early promoters of P2 vir3, vir3 corll, vir24, and vir24 cor3l mutants, each promoter was cloned in front of a promoterless cat gen[r] ...
5
Binding of EBNA-1 to DNA creates a protease-resistant domain that encompasses the DNA recognition and dimerization functions.
... Protease digestion generates an 18-kDa DNA-binding domain that maps within the aa 459 to 617 segment of EBNA-1, as determined by gel electrophoretic analysis of the protease-resistant EB[r] ...
8
Separation of the complex DNA binding domain of EBNA-1 into DNA recognition and dimerization subdomains of novel structure.
... These mutants plus the availability of a new monoclonal antibody against EBNA-1, EBNA.OTlx, allowed us to separate the individual DNA binding and dimerization domains and to gain some in[r] ...
11
Key determinants of target DNA recognition by retroviral intasomes
... B-form DNA, it seems possible that significantly less deformation is required to fit chromosomal DNA into the two active sites of a viral intasome that generates a 6 bp ...
19
Ramanjaneyulu, Allam (2010): The role of cytosolic RNA and DNA recognition in systemic autoimmunity and immune complex glomerulonephritis: Cytosoli RNA and DNA recognition in autoimmunity. Dissertation, LMU München: Medizinische Fakultät
... Cells were harvested for RNA isolation after stimulation with different ligands using RNeasy Mini Kit, according to the manufacture protocol. Cells were washed with sterile PBS to remove residual medium. 350 μl RLT ...
117
Thiazotropsin aggregation and its relationship to molecular recognition in the DNA minor groove
... Molecular recognition between the DNA minor groove and classical small molecule DNA binders including Hoechst 33258 [1-234567] berenil [8,9] netropsin [10] distamycin A [11,12] and their derivatives ...
37
DNA loci cross talk through thermodynamics
... The recognition and pairing of specific DNA loci, though crucial for a plenty of important cellular processes, are produced by still mysterious physical ...some DNA recognition sequences, ...
9
Towards a comprehensive system for the recognition of double helical DNA : components for T and A
... the recognition o f a sixteen base pair ...in DNA recognition, as a m inim um o f 16-17 base pairs are necessary to specify any site within the hum an genom ...
198
Mechanism of transcription-coupled DNA modification recognition
... and DNA repair protein complexes, as well as to induce transient pausing of pol II in vitro [21, ...‘epi- DNA recognition loop’, located in the fork region of the Rpb2 subunit of pol II, is ...
5
Electrochemical Studies on the Recognition of a Ternary Copper Complex to Single-Stranded DNA and Double-Stranded DNA
... to DNA with different structures has attracted much attention in the field of DNA ...of DNA detection [1-3], indicators of DNA hybridization detection [4-6] and chemical nuclease ...different ...
14
Recognition of Cytosolic DNA Attenuates Glucose Metabolism and Induces AMPK Mediated Energy Stress Response
... viral DNA recognition on AMPK-mTORC1 ...viral DNA recognition attenuates glucose metabolism leading to activation of AMPK and inhibition of mTORC1, which plays a pivotal role in cell growth ...
8
Serological survey of Leishmaniainfection in blood donors in Salvador, Northeastern Brazil
... Leishmania DNA in 8/38 (21%) and 28/38 (68%) seropositive samples, respect- ively, and agreement between both techniques was fair (kappa = ...Leishmania DNA by ...
8
Frauer, Carina (2010): Studies of mechanisms controlling DNA methyltransferase 1. Dissertation, LMU München: Fakultät für Biologie
... Next, we investigated the role of distinct Np95 domains in nuclear interactions in vivo. To this aim, we expressed the same GFP–Np95 constructs in np95 / ESCs and tested their binding kinetics with FRAP experiments ...
190
Retroviral integration: in vitro host site selection by avian integrase.
... The combined results of the lambda and oligonucleotide assays indicated that although host site selection has properties in common with recognition of the viral DNA termini, the nonrando[r] ...
8
Organization and function of the phage Lambda chromosome
... missing DNA to the right of XOP. Since these three deletion types all displayed non-complementable integration defects, it was proposed. that attcp contains recognition sequences on [r] ...
216
DNA substrate recognition and processing by the full-length human UPF1 helicase
... Although hUPF1 is recognized principally as a 5 –3 RNA helicase, where the enzyme engages with a 5 single- stranded NA tail and translocates upon it during strand displacement (19), we used a partially single- and ...
13
Recognition and Determination of DNA Using Victoria Blue B as Electrochemical Probe
... Differential pulse voltammetric method (DPV) was chosen for analytical application because it is more sensitive than cyclic voltammetric method. The differential pulse voltammograms of VBB in the absence and presence of ...
12