DNA samples
Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV like sequences
... WPI-1282 DNA, equivalent to <1 cell, when mixed with 200 ng control DNA (see above) (Figure ...test DNA in triplicates of 200 ng each from our CFS and control ...of DNA samples from ...
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New Statistical Tests of Neutrality for DNA Samples From a Population
... for determining the critical values of these tests are quite successful. The advantage of this approach is that it can bring the achieved levels of significance close to [r] ...
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Quality of DNA extracted from saliva samples collected with the Oragene™ DNA self-collection kit
... • DNA/saliva mixed samples and the second one using the remaining volume of mixer in the collection ...and DNA precipitation with 100% ethanol. The DNA was rehy- drated in 300 μL of TE ...
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Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping
... Saliva DNA samples were plated out in dupli- cate on a 384 well plate, alongside additional duplicate DNAs taken from the paired blood samples (all normal- ised to 2 ...
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Clinical trial participant characteristics and saliva and DNA metrics
... and DNA was precipitated by adding an equal volume of 100% ethanol. The DNA pellet was washed with 70% eth- anol, dried, and resuspended with DNA hydration solu- tion (Qiagen, Valencia, ...CA). ...
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A quick and cost effective method for the diagnosis of Mycobacterium ulcerans infection
... purified DNA samples are not a require- ment. DNA amplification occurs within 1 hour and the resulting product is a turbid solution, indicative of pro- duct ...
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Maryland v. King and the Road Already Traveled: How the United Kingdome Tried--and Failed--to Balance State Interests with Privacy Rights
... intimate samples included “a) ...one’s DNA profile contains much more information about oneself than does a dental impression or even a fingerprint, since dental impressions and fingerprints can only ...
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Saliva sampling in global clinical studies: the impact of low sampling volume on performance of DNA in downstream genotyping experiments
... saliva DNA samples performed similarly to blood DNA samples, a greater proportion of saliva DNA sam- ples were likely to fail genotyping QC standards and that samples where the ...
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DNA damage in preserved specimens and tissue samples: a molecular assessment
... on DNA labeling with FAM-ddATP and Terminal ...the DNA extract have a free 3'-hydroxyl group, but we assume the underlying mechanisms of fragmentation to be sequence independent, but see for example ...the ...
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Analysis of multiple single nucleotide polymorphisms closely positioned in the ovine PRNPgene using linear fluorescent probes and melting curve analysis
... blood samples. Blood samples only need to be diluted in water to reduce PCR inhibition and lighten sample colour enabling fluorescence ...blood samples due to the low DNA concentrations once ...
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Interlaboratory Evaluation of Different Extraction and Real Time PCR Methods for Detection of Coxiella burnetii DNA in Serum
... fore, DNA sequence polymorphisms in the IS1111a elements in this Dutch clone may also affect the different PCR assays studied here and may also explain the poor performance of the PCR used by laboratory ...in ...
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“Genetic Variability Studies of Clinical Isolates of Lower Respiratory Pathogen Klebsiella pneumonia” by T.P.Kumari Pushpa Rani, S.K.Sundar, B. Vijayalakshmi Amma, India.
... Genomic DNA was isolated from twenty five clinical isolates of Klebsiella pneumoniae and the DNA was separated in Gel electrophoresis to assess their ...isolated DNA samples of the twenty five ...
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Noninvasive detection of tumor-associated mutations from circulating cell-free DNA in hepatocellular carcinoma patients by targeted deep sequencing
... The relevant mutation sites that we designed were detected in DNA samples from plasma and matched tumor tissue. The sequence depth of the targeted regions is shown in Figure 1. We detected and captured ...
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A review on isolation of dna from botanical samples and their dna fingerprinting
... genomic DNA from fresh and dry leaves of Vigna aconitifolia and ...The samples were divided into two- one was kept at 4 o C and the other was dried at 50 o ...leaf samples were crushed in a ...
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Neutrality Tests Using DNA Polymorphism From Multiple Samples
... The polymorphism of a gene or a locus is studied with increasing frequency by multiple laboratories or the same group at different times. Such practice results in polymorphism being revealed by different samples ...
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semi-quantitative estimation of wheat root DNA in soil samples
... biomass of field grown wheat varieties and black-grass using root DNA extracted from soil.. 112.[r] ...
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Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products
... cell DNA quantita- tion [14, ...residual DNA quantitation due to their sensitivity, accuracy, precision, and time- saving ...residual DNA of Escheri- chia coli, NS0, yeast, and Chinese hamster ...
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Evaluation of Use of Epstein Barr Viral Load in Patients after Allogeneic Stem Cell Transplantation To Diagnose and Monitor Posttransplant Lymphoproliferative Disease
... The EBV load as an indicator for an EBV-associated disease parallels some aspects of CMV disease. CMV disease is asso- ciated with a high viral load, yet patients with high viral loads do not necessarily develop CMV ...
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Efficacy of Bisulfite Modification and DNA Recovery Using Commercial Kits from Samples of Genomic and Circulating DNA
... EZ DNA Methylation-Gold Kit from Zymo Research provided the highest yield of DNA after bisulfite treatment and isolation; the mean yield of DNA was no less than ...of DNA recovery after ...
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Ancient DNA analysis of Māori feather cloaks and kete : implications for conservation and culture : a thesis presented in fulfilment of the requirements for the degree of Doctor of Philosophy in Molecular Biosciences at Massey University, Auckland, New Zealand, 2012
... cloak samples was 70%, this varied greatly between the museums where the cloak samples came ...Kingdom. DNA begins to degrade the moment it is no longer part of a living ...to DNA degradation. ...
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