DNA sequence analysis vs. Multi-locus fingerprinting
DNA fingerprinting of Candida rugosa via repetitive sequence based PCR
... DISCUSSION The objective of this study was to develop a rep-PCR-based fingerprinting technique to discriminate among different C. rugosa isolates from burn patients and apply it to other Can- dida species. The ...
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Dna Fingerprinting Analysis Worksheet Answer Key
... the fingerprinting answer key included on the base pairs. Its charge is dna fingerprinting analysis worksheet answer key included on a light box, not an identical genetic engineering because ...
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Multi-locus sequence type analysis of Shigellas pp. isolates from Tehran, Iran
... five sequence types with ST 152 as the dom- inant sequence type being composed of ...among sequence types for better prevention, control and treatment of ...
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Multi locus sequence typing of oenological Saccharomyces cerevisiae strains
... In this work we used MLST analysis for molecular typing of several 89.. cerevisiae wine yeast strains, including isolates from sherry and sparkling wines, 90.[r] ...
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COMPARISON OF DNA FINGERPRINTING ANALYSIS FOR IDENTIFICATION OF BACILLUS SPECIES
... By 16S rRNA gene sequence analysis, the isolates used for the study were identified as Bacillus. The results of sequencing matched with that of the fingerprinting analysis upto species level. ...
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Ecological aspects of multi-locus variability of DNA markers in animals
... INTRODUCTION I t is known, that Shmalkhausen [1] calls allelic diversity as “mobilization reserve” of the population, which provides it with a more stable existence in time. Based on this view, it is possible to assess ...
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Multi-locus sequence analysis of Mycoplasma carpicolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia
... on Multi-Locus Sequence Analysis (MLSA) has been developed to trace new epidemics and to elucidate whether the recently identified cases in continental Asia were due to recent importation of ...
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Multi-locus sequence analysis of mycoplasma capricolum subsp. capripneumoniae for the molecular epidemiology of contagious caprine pleuropneumonia
... H2 locus, a polymorphic region already in use as a molecular marker for Mccp evolution, was complemented with seven new loci selected according to the analysis of polymorphisms observed among the genome ...
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DNA fingerprinting of Shiga-toxin producing Escherichia coli O157 based on Multiple-Locus Variable-Number Tandem-Repeats Analysis (MLVA)
... repeated DNA motifs with the Tandem Repeat Finder [26], GeneQuest (DNASTAR, Madison WI) and Kodon (Applied-Maths, Kortrijk, Belgium) ...tailing sequence of 500 bps were used to design specific PCR primers ...
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Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
... the DNA. The DNA was se- quenced by the Oklahoma State University Recombinant DNA/Protein Core facility using a DNA analyzer (ABI Model 3730; Applied ...resulting DNA sequences were ...
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Multi Locus sequence analysis reveals profound enetic diversity among isolates of the human athogen bartonella bacilliformis
... AFLP analysis and comparison of ISR ...bvrR locus, and was verified by repeat amplification and sequencing of this ...non-ST8 sequence types, ST2, of which Cond044 was the sole representative, was ...
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Multi Locus Sequence Typing scheme for Acidithiobacillus caldus strain evaluation and differentiation
... copy genes sequences using a flexible sliding window ranging in size from 250 to 650 bp and established the putative forward and reverse PCR primer amplification target sites on each marker setting a restriction for ...
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A Fast, Accurate, and Automated Workflow for Multi Locus Sequence Typing of Bacterial Isolates
... Data analysis in the current MLST workflow requires 4–5 hours of post-analysis data retrieval and processing of each ...imal sequence checking, the ST of each sample can be obtained in five minutes ...
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DNA Fingerprinting of Lactobacillus crispatus Strain CTV 05 by Repetitive Element Sequence Based PCR Analysis in a Pilot Study of Vaginal Colonization
... whether DNA fingerprinting by repetitive element sequence-based PCR (rep-PCR) can be used to distinguish the capsule strain of ...the DNA fingerprints obtained were ...unique DNA ...
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DNA fingerprinting of Vibrio cholerae strains with a novel insertion sequence element: a tool to identify epidemic strains
... related strains may express different serotypes is consistent with the transfer of cell wall polysaccharide genes in V. chol- erae. Characterization of serotype O37 strains S7 and G12R from Sudan. As described above, O37 ...
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Multilocus Sequence Typing Is a Reliable Alternative Method to DNA Fingerprinting for Discriminating among Strains of Candida albicans
... RAPD analysis and was essentially as discriminating as MLEE and Ca3 Southern hybridization, with Ca3 Southern hybridization usually being accepted as the most discriminating of the image-based techniques and as ...
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Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes
... and analysis of rep-PCR products were implemented using the DiversiLab system, in which the amplified fragments of various sizes and fluorescence intensities were separated and detected using a microfluidics chip ...
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Comparison of classical multi locus sequence typing software for next generation sequencing data
... (8) and scalability to large numbers of isolates. The interfaces to the software applications fall into two cate- gories, those that operate on the command line and those that have a graphical interface. Command line ...
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Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe
... We conclude that for a well-defined subset of hospitalized patients who were colonized by Candida species before developing nosocomial candidemia, the colonizing and infecting strains we[r] ...
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DNA sequence variability at the rplX locus of Bacillus subtilis
... Even though the number of variable sites is small, they are sufficient to suggest that the relationships among these rplX sequences are different from those inferred [r] ...
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