DNA single-strand breaks
The generation of DNA single-strand breaks during the reduction of chromate by ascorbic acid and/or glutathione in vitro.
... With relatively high amounts of chromate (500 PM) however, the combina- tion of AsA and GSH exhibited a slightly protective influence on the generation of strand breaks; the cleavage obs[r] ...
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Evaluation of DNA Integrity of Cryopreserved Boer Goat (Capra hircus) Sperm Using Comet Assay at Various pH Conditions
... double-strand DNA breaks (neutral comet assay) were significantly higher than single-strand DNA breaks (alkaline comet ...double-strand DNA breaks ...
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Efficient Repair of DNA Breaks in Drosophila: Evidence for Single-Strand Annealing and Competition With Other Repair Pathways
... ment B had undergone SSA at 50C yet had colored eyes. Figure 1.—Proposed mechanism for the formation of dupli- DNA sequencing: The sequence of the 1290-bp repeats was cations (Preston et al. 1996) and collapse by ...
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Effects of DNA Double-Strand and Single-Strand Breaks on Intrachromosomal Recombination Events in Cell-Cycle-Arrested Yeast Cells
... In DNA DSBs may be involved in these DEL recombina- strain AGY 26A (w/o M13ori) on galactose only a nonsig- tion events ( Galli and Schiestl ...on DNA damage, repair and recombination pro- galactose, did ...
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Replication Protein A Is Required for Meiotic Recombination in Saccharomyces cerevisiae
... transient DNA double-stranded breaks ...3⬘ single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair ...the strand exchange ...the DNA-binding ...
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Capture of DNA Sequences at Double-Strand Breaks in Mammalian Chromosomes
... Inserted into the tk gene is a 24-bp oligonucleotide (hatched electroporation cells were fed with DMEM supplemented with segment) containing the 18-bp recognition site for yeast endo- hypoxanthine/aminopterin/thymidine ...
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Oxidative stress, activity behaviour and body mass in captive parrots
... and DNA; measurement of malondialde- hyde (MDA), a product of lipid peroxidation, is one of the most commonly used techniques (Young and Trimble, ...gauged DNA damage because it is estimated that ROS are ...
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Predicting double strand DNA breaks using epigenome marks or DNA at kilobase resolution
... at single- nucleotide ...Endogenous breaks were captured for normal human epidermal keratinocytes (NHEKs), for which numerous ChIP-seq and DNase-seq data are publicly available from the ENCODE project ...
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PubMedCentral-PMC5041911.pdf
... oxidative DNA damage, mammalian cells possess DNA repair systems that serve to prevent and repair ROS-induced ...these DNA repair pathways in cells do not possess high fidelity, leading to genomic ...
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Antigenotoxic properties of Paliurus spina christi Mill fruits and their active compounds
... PS is traditionally used as diuretic, antirheumatic, hypocholesterolemic and tonic as well as in inflamma- tion, diarrhea, and chronic obstructive pulmonary disease [7]. Its water extract and their compounds show ...
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The Role of the Ku70 vWA Domain in the Response to DNA Double Strand Breaks
... for DNA end binding. The binding of HR factors initiates DNA end resection to produce single-stranded DNA, which Ku does not have a strong affinity for, and promotes the completion of DSB ...
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Early neuronal accumulation of DNA double strand breaks in Alzheimer’s disease
... Immunostained cultures were imaged with a high-con- tent ArrayScan system (Thermo Fisher Scientific) and a 40x objective, yielding 1024 × 1024 pixels per image. For each well, 15–20 images were obtained from different ...
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DNA Damage-Inducible and RAD52-Independent Repair of DNA Double-Strand Breaks in Saccharomyces cerevisiae
... homologous recombination, end joining requires little It is generally accepted that cell cycle arrest ensures or no DNA homology, and thus is also called nonhomol- time for DNA repair before cells reenter ...
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Sp1 phosphorylation by ATM downregulates BER and promotes cell elimination in response to persistent DNA damage.
... of DNA repair systems. Amongst these, the DNA base excision repair (BER) pathway constitutes the front- line defense against endogenously-generated DNA damage including DNA base lesions and ...
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Studies on DNA Topology and DNA-Amyloid Beta (Aβ) peptides interaction in relevance to Alzheimer's Disease
... SAD DNA were fragmented as evidenced by their smear migration ...SAD DNA as both of them have smeared pattern, EtBr staining is faint for SAD ...to DNA isolated from other regions of the brain, ...
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Increased single strand breaks in DNA of lymphocytes from diabetic subjects
... glycohemoglobin values above or below 9%. The group with glycohemoglobin values of 12.9 +/- 2.4% (mean +/- SD), but not the group with glycohemoglobin values of 7.4 +/- 1.5%, showed accelerated unwinding of lymphocyte ...
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Trabectedin triggers direct and NK-mediated cytotoxicity in multiple myeloma
... specific DNA damages. Cur- rently, at least seven DNA repair active systems have been described in MM as protection from different DNA lesions ...of single-strand DNA damages; ...
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“Breaking news” from spermatids
... to DNA damage and DNA double-strand breaks (DSBs) are observed ...minated DNA adopts a completely different topology as the somatic-like DNA supercoiling is lost and planar ...
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Molecular Mechanisms of H. pylori Induced DNA Double-Strand Breaks
... oxidative DNA damage leads to DSBs repair oxidative damaged DNA via BER, which is thought to be the primary repair pathways against oxidative DNA ...
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Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos
... on DNA, as observed through the accumulation of CPD photoproducts, and the positive TUNEL assay, which indicates the occurrence of DNA strand breaks caused by ROS (Imlay and Linn, 1988; ...
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