DNA stable-isotope probing
Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N DNA stable isotope probing
... Bending and Georg Cadisch Article Title: Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N-DNA stable isotope probing Year of p[r] ...
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Proteomic Stable Isotope Probing Reveals Taxonomically Distinct Patterns in Amino Acid Assimilation by Coastal Marine Bacterioplankton
... technique, DNA stable isotope probing (DNA-SIP), applies metagenomic approaches to whole- community DNA that is separated based on differences in density due to isotopic label ...
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A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments
... Additional file 6: Figure S1. Microcosm incubation of salt marsh sediments with trimethylamine (TMA). Samples were taken at 234 hr (T1), 307 hr (T2) and 427 hr (T3) for amplicon sequencing of microbial 16S rRNA genes. ...
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Consuming algal products: trophic interactions of bacteria and a diatom species determined by RNA stable isotope probing
... (Eppendorf), 300 lM of each dNTP (PerkinElmer), 0.2 lM of each primer, 2 U Taq DNA Polymerase (Eppendorf), and 20 ll RT-reactions. The touchdown PCR started with a denaturing step at 94°C for 5 min. Every cycle ...
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Stable Isotope Studies of Fuel Oxygenate Biodegradation.
... analysis, DNA-SIP, and RNA-SIP) for stable isotope probing analysis have become widely applied in studies focused on biodegradation of environmental ...
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Novel bacteria capable of degrading phenanthrene in activated sludge revealed by stable isotope probing coupled with high throughput sequencing
... encoding PAH-RHDs from Shewanella sp. (KU372133.1), Pseudomonas sp. (AM743143.1) and uncultured bacterium (KF155785.1 and KX364042.1) and NDOs from Comamonas sp. (JN655512.1) and Burkholderia sp. (AF448048.1) (Fig. 5). ...
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Stable-isotope probing-based investigations of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated soil
... multiple DNA extractions on soil samples improves extractable DNA yield and quantifiable eubacterial 16S rRNA gene copies, but has little qualitative effect on the identification of the bacterial groups ...
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Characterization of para nitrophenol degrading bacterial communities in river water using functional markers and stable isotope probing
... of pnpA and mar sequences. A standard curve of a fivefold dilution of each plasmid was made from 0.002-800 pg/μl. QPCR reactions for experimental samples, standards and blank were performed in triplicate. An average of ...
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Effect of incubation conditions on the enrichment of pyrene-degrading bacteria identified by stable isotope probing in a PAH-contaminated soil
... Parallel FW microcosms containing unlabeled pyrene yielded more concentrated total community DNA extracts than slurry microcosms. A PCR for DGGE was performed using these extracts as template. The resulting ...
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Stable isotope probing of salicylate-degrading bacteria in polycyclic aromatic hydrocarbon-contaminated soil
... to stable-isotope labeling include DNA, RNA, and ...at DNA (Radajewski et ...than DNA (Manefield et al. 2002b). Reports of stable-isotope labeling of lipids have also ...
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APPLICATION OF STABLE ISOTOPE SIGNATURES IN FOOD TRACEABILITY
... N > 4‰) that signify the organic fertilizer usage. Generally, nitrogen fraction in nature are due to kinetic effects of non-biological and biological effect. There are two non-biological fration effects, dissolution ...
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New Identity of the Kimberlite Melt: Constraints from Unaltered Diamondiferous Udachnaya East Pipe Kimberlite, Siberia, Russia
... Radiogenic isotope compositions (Nd, Sr, and Pb) of the chloride, chloride - alkali carbonate, carbonate and oxide-silicate constituents in the groundmass of the Udachnaya-East kimberlite effectively show the ...
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Relative and Absolute Dating Methods (CTG)
... • The half-life of a radioactive isotope is the time it takes for one half of the atoms of the original unstable “parent isotope” to decay to atoms of a new more stable “daughter. is[r] ...
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Speleothem stable isotope records from Eastern
... stable carbon and oxygen isotope ratios of carbonate deposits became the most frequently.. 78.[r] ...
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Hagfish feeding habits along a depth gradient inferred from stable isotopes
... Acknowledgements. The MV ‘Tranquil Image’ crew N. Fur- ley, G. Gibbs and S. Kelly helped to organize all of the field- work using baited fish traps. R. Crech’riou, A. Smith, C. Bed- ford, O. Hannaford, K. Rodgers, C. ...
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STABLE ISOTOPES REVEAL A DISCONNECT BETWEEN BIOTIC AND ABIOTIC HYDROLOGICAL PROCESSES IN A SEASONALLY-DRY, SEMI-ARID WATERSHED
... water isotope values plot significantly to the southwest of their March plotting locations, indicating that these plants have taken up new ...water isotope values, below the lower 95% confidence interval ...
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2015-halffman-potter-etal
... Bone Collagen Extraction and Stable Isotope Methods. Bone colla- gen was extracted from a single unburned salmonid vertebra (58- 30) for carbon and nitrogen stable isotope analysis. The spec- ...
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Continental carbonate facies of a Neoproterozoic panglaciation, north east Svalbard
... Carbon and oxygen stable isotope data are presented here as δ13C and δ18O in parts per... and other isotope analyses will be presented elsewhere..[r] ...
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Trophic dynamics of scleractinian corals: stable isotope evidence
... of stable isotope signature and lipid composition of the tissue of red sea coral species, which have suggested a high efficiency of carbon recycling between the symbiotic partners (Alamaru et ...
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Stable isotopes and dynamic diets : the Mesolithic-Neolithic dietary transition in terrestrial Central Europe
... The Mesolithic-Neolithic transition in Europe marks a distinct change in human food production, typically characterised as the transition from hunting and gathering to farming. Meat from domesticated animals and ...
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