DNA strand annealing activity
Effects of Mutations of RAD50, RAD51, RAD52, and Related Genes on Illegitimate Recombination in Saccharomyces cerevisiae
... Because there is no long regions of homology at the recombination junc- tion sites, it is unlikely that its DNA strand transfer activity and/or DNA annealing activit[r] ...
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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania
... gRNA activity in Leishmania, the actual activity rank order of the MLF resistance rates for these gRNAs (gRNAf ⬎ gRNAe ⬎ gRNAd) was consistent in these Leishmania ...different activity observed for ...
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Dynamic DNA strand displacement circuits
... the DNA molecule via an NHS ester), and RQ denotes the Iowa Black Red ...Catalytic activity of the allosteric ...by annealing AC with a 3x excess ...
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Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase
... facilitate DNA strand ...ssDNA annealing and why mutations in the active ssDNA binding channel can enhance annealing activity awaits further structural ...forked DNA substrates ...
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Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase
... facilitate DNA strand ...ssDNA annealing and why mutations in the active ssDNA binding channel can enhance annealing activity awaits further structural ...forked DNA substrates ...
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GKE122.pdf
... by annealing a 30mer oligonucleotide to a ...single-stranded DNA overhang of 15 nt referred to as the bound ...15mer DNA was replaced by a strand of peptide nucleic acid ...of strand ...
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Nucleic Acid Chaperone Activity Associated with the Arginine-Rich Domain of Human Hepatitis B Virus Core Protein
... chaperone activity of full-length HBc by performing both DNA annealing ...and strand exchange (displacement) ...and strand annealing, which could be analyzed by the strand ...
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Molecular Requirements for Human Immunodeficiency Virus Type 1 Plus-Strand Transfer: Analysis in Reconstituted and Endogenous Reverse Transcription Systems
... the annealing of (1) SSDNA to the 18-nt sequence at the 39 end of the tRNA and to the minus-strand PBS sequence in the acceptor DNA tem- plate as a strand exchange ...the DNA duplex ...
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Homologous Recombination via Synthesis-Dependent Strand Annealing in Yeast Requires the Irc20 and Srs2 DNA Helicases
... mediator activity of Rad52 for the Rad51-catalyzed D-loop formation between an RPA-coated single-strand tail and a homologous double-strand DNA (Sung ...Srs2 DNA helicase was sug- ...
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Annealing of Complementary DNA Sequences During Double-Strand Break Repair in Drosophila Is Mediated by the Ortholog of SMARCAL1
... ATP-driven annealing activity called annealing helicases (Yuan et ...single DNA strands, making these enzymes ideal candidates for annealing during ...replication-associated DNA ...
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Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures
... a DNA duplex of noncomplementary strands; to our knowledge, this ar- rangement is the only such representation in the ...catalyze DNA strand annealing suggest that this arrangement is not ...
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Mutations That Increase In Situ Priming Also Decrease Circularization for Duck Hepatitis B Virus
... hepatotropic DNA viruses that carry out genome replication via reverse transcription of an RNA intermediate, the pregenome (for a review, see ref- erence ...first- strand, or minus-strand, synthesis ...
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Replication Protein A Is Required for Meiotic Recombination in Saccharomyces cerevisiae
... genomic DNA was extracted from RFA1 (ORD- 2130), rfa1-t11 (ORD3256), rfa1-t48 (ORD5402), rad51 (ORD3200), rad55 (ORD- 3204), and rad52 (ORD- 3285) strains and digested with the restriction enzymes MluI and ...
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DNA strand exchange and selective DNA annealing promoted by the human immunodeficiency virus type 1 nucleocapsid protein.
... 9 DNA Strand Exchange and Selective DNA Annealing Promoted by the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein ZENTA TSUCHIHASHIt AND PATRICK 0.. BROWN* Howard Hughes Medical[r] ...
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“MODERN ASPECTS FOR ANTIRETROVIRAL TREATMENT” by Viraj S. Saste*, Sachin S. Kale, Mangesh K. Sapate, Dheeraj T. Baviskar, India.
... nanoparticles employed as a platform (2.0 nm diameter, mercaptobenzoic acid modified gold particles) transformed a weakly binding and biologically inactive small molecule into a multivalent conjugate that effectively ...
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Probability of Genetic Diagnosis
... the DNA molecule, we conduct the calculation according to the results obtained ...of DNA as a sample space,and selecting a sample of DNA ...use DNA molecular to compute Bayesian ...
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Sequence identity of the terminal redundancies on the minus-strand DNA template is necessary but not sufficient for the template switch during hepadnavirus plus-strand DNA synthesis.
... synthesize DNA (for a monograph, see reference ...or strand transfer. Because initia- tion of both minus- and plus-strand DNA synthesis usually occurs at an internal position on the template ...
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Studies on DNA Topology and DNA-Amyloid Beta (Aβ) peptides interaction in relevance to Alzheimer's Disease
... the DNA nicking caused various peptides and are differentially modulated by ...metals. DNA nicking of A was inhibited by aurintricarboxylic acid (ATA), a potent nuclease ...of DNA nicking by metals ...
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Evaluation of DNA Integrity of Cryopreserved Boer Goat (Capra hircus) Sperm Using Comet Assay at Various pH Conditions
... double-strand DNA breaks of the sperm for the simple reason that the neutral comet assay is a sensitive and simple technique of evaluating the double-strand DNA damage of the sperm ...the ...
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Efficient initiation and strand transfer of polypurine tract-primed plus-strand DNA prevent strand transfer of internally initiated plus-strand DNA.
... in DNA samples digested with NcoI plus NotI. When DNA samples were digested with NcoI alone, only the ...that DNA initiated from the 5 9 PPT was rarely if ever used for strand ...of DNA ...
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