DNase I
StpA protein from Escherichia coli condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI
... to DNase I attack with increasing StpA ...of DNase I and thereby preventing digestion, this was unlikely owing to the low concentrations of StpA (256 bp: StpA) required to inhibit DNase ...
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Tracking chromatin states using controlled DNase I treatment and real-time PCR
... Reagents were purchased from Amresco Inc. (Solon, OH). All buffers and solutions were freshly-prepared excluding Frozen Storage Buffer, FSB [9], composed of 50 mM HEPES buffer pH, 7.5, containing 10 mM sodium chloride, 5 ...
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Significance of DNase I-hypersensitive sites in the long terminal repeats of a Moloney murine leukemia virus vector.
... Several weak DNase I HS sites are present in the cellular sequences adjacent to the proviral integration sites of the infected cell lines IF-1 and IF-2, but not in the transfected cell l[r] ...
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Transcriptional activation and DNase I hypersensitive sites are associated with selective expression of the gastrin releasing peptide gene
... four DNase I hypersensitive ...of DNase hypersensitive sites in the promoter region of the GRP gene is the structural feature that best correlates with transcriptional ...four DNase ...
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Methylation state and DNase I sensitivity of chromatin containing Moloney murine leukemia virus DNA in exogenously infected mouse cells.
... For the clone 80 integration lacking DNase I-hypersensitive sites, the methylation state and DNase I sensitivity of the adjacent cell DNA as well as in the host site without integrated p[r] ...
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DNase I hypersensitivity analysis of the mouse brain and retina identifies region-specific regulatory elements
... whether DNase I hypersensitivity mapping can discover regulatory elements associated with expression of genes specific to particular regions of the CNS, we focused on an analysis of the neural retina, for ...
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Acceptor sites for retroviral integrations map near DNase I-hypersensitive sites in chromatin.
... Positions of unselected proviral integrations and DNase I-hypersensitive HS sitesa Total length of Position of HS Position of proviral DNA analyzed Locus sites kb integration kb kb dsi-3[r] ...
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Improved DNase-seq protocol facilitates high resolution mapping of DNase I hypersensitive sites in roots in Arabidopsis thaliana
... The past use of low-melting agarose plugs in combina- tion with a more vigorous nuclei isolation protocol [6, 10] made it possible to analyze DHSs in leaf and flower tis- sue in Arabidopsis and seedling and callus tissue ...
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Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns.
... DNase I footprinting 17 of both the HTLV-I and HTLV-II LTRs with extracts prepared from HeLa cells, uninfected T lymphoid cells, and HTLV-I- and HTLV-II-transformed lymphoid cells indica[r] ...
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Isolation of episomal bovine papillomavirus chromatin and identification of a DNase I-hypersensitive region.
... 2 0022-538X/83/050567-08$02.00/0 Copyright C 1983, American Society for Microbiology Isolation of Episomal Bovine Papillomavirus Chromatin and Identification of a DNase I-Hypersensitive [r] ...
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Distribution of DNase I-sensitive sites in simian virus 40 nucleoprotein complexes from disrupted virus particles.
... Alternatively, the nucleoprotein Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Nucleoprotein complexes core particles released from simian virus 40 SV40 virions were [r] ...
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In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
... Alterna- tively, the -20.5 kb DHS, which is present in pancreatic chromatin from the transgenic mice, might be involved in the low-level expression detected in the transgenic mouse pancr[r] ...
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Transcription of the S gene in transgenic mice is associated with hypomethylation at specific sites and with DNase I sensitivity.
... 931 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The methylation status of hepatitis B virus HBV DNA was investigated in different organs from two strains of transge[r] ...
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The Gypsy Insulator of Drosophila Affects Chromatin Structure in a Directional Manner
... 5.—DNase I accessibility analyses of the yellow locus in mod(mdg4) ...the DNase I hypersensitive sites is shown on the right of A and B; the yellow gene is shown as a thin line, the gypsy ...
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Neutrophil extracellular traps impair intestinal barrier function during experimental colitis
... or DNase I- treated C57BL/6 mice were given ...of DNase I, which dissolved the NET structure in vivo, resulted in a significantly reduced recovery of FITC-dextran from the serum of DSS-treated ...
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Mechanism of Degradation of Duplex DNA by the DNase Induced by Herpes Simplex Virus
... HSV-I specific DNA polymerase activity 0 and the release of acid-soluble nucleotides A were measured for calf thymus DNA which had been treated for the indicated times with A DNase I, B [r] ...
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Nuclease-sensitive sites in the two major intracellular simian virus 40 nucleoproteins.
... The 200S fraction was treated to release core particles that were sensitive to nuclease cleavage; however, DNase I showed little or no preference for the 0.66 to 0.73 region of the provi[r] ...
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Stabilisation of TG-and AG-containing antiparrallel DNA triplexes by triplex-binding ligands
... inhibit DNase I cleavage, with bands still evident within the footprints at high oligonucleotide ...for DNase I. Since DNase I cuts from the minor groove, while triplex-forming ...
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Multiple protein-binding sites in an intracisternal A particle long terminal repeat.
... Three of the DNase I footprints include a sequence with homology to the simian virus 40 core enhancer and sequence motifs that closely resemble the binding sites for transcription factor[r] ...
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Mutational analysis of the trans-activation-responsive region of the human immunodeficiency virus type I long terminal repeat.
... An analysis of the DNase I hypersensitivity profile of the HIV-I LTR in transfected cultures suggested the presence of at least two DNase I-hypersensitive sites, including one which exte[r] ...
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