dominant negative
Dominant negative mutant Cyclin T1 proteins inhibit HIV transcription by specifically degrading Tat
... strongest dominant nega- tive effect (>90% inhibition) on HIV transcription in HeLa/HR-Luc cells ...potent dominant negative effects on HIV transcription (60–90%, data not Construction of mutant ...
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Dominant-Negative Inhibition of Prion Formation Diminished by Deletion Mutagenesis of the Prion Protein
... be dominant negative and has been simulated in cultured cells persistently infected with scrapie ...as dominant-negative, inhibitors of PrP Sc formation ...
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Dominant-negative activity of the STAT3-Y705F mutant depends on the N-terminal domain
... how dominant-negative activity of STAT3-YF- YFP depends on the subcellular localization, we gener- ated a STAT3-YF-YFP construct with a NLS sequence ...The dominant-negative activity of ...
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A recurrent dominant negative E47 mutation causes agammaglobulinemia and BCR– B cells
... expression vectors. E555K in the transfected cells localized normally to the nucleus and resulted in a dominant negative effect when bound to DNA as a homodimer with wild-type E47. Mutant E47 did permit DNA ...
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Regression/Eradication of gliomas in mice by a systemically- deliverable ATF5 dominant-negative peptide
... Malignant gliomas have poor prognosis and urgently require new therapies. Activating Transcription Factor 5 (ATF5) is highly expressed in gliomas, and interference with its expression/function precipitates targeted ...
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Dominant-Negative FADD Rescues the In Vivo Fitness of a Cytomegalovirus Lacking an Antiapoptotic Viral Gene
... a dominant-negative mutant of FADD, a molecular junction in the death-receptor-mediated pathway of apoptosis, can functionally replace a viral antiapop- totic ...of dominant-negative genes ...
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Mutational Analysis of Bovine Leukemia Virus Rex: Identification of a Dominant-Negative Inhibitor
... of dominant-negative mutant proteins that represent promising antiviral ...viral dominant-negative proteins to prevent or treat viral infections in ...
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Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
... Inactivation of gene products by dominant-negative (DN) mutants is a powerful tool to assign functions to proteins. Here, we present a two-step procedure to establish a random screen for DN alleles, using ...
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The dominant-negative interplay between p53, p63 and p73: A family affair
... were dominant-negative in our ...the dominant-negative capacity of ∆N-p73α and ∆N-p73β may be due to the formation of inactive hetero-tetramers with functional isoforms of p63 and p73 and/or ...
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Dominant Negative Inhibition of Human Immunodeficiency Virus Particle Production by the Nonmyristoylated Form of Gag
... Myristoylation of human immunodeficiency virus (HIV) Gag protein is essential for membrane targeting of Gag and production of viral particles. We show here that coexpression of wild-type and nonmyristoylated forms of HIV ...
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Inhibition of Elastase-Pulmonary Emphysema in Dominant-Negative MafB Transgenic Mice
... of the Maf protein family and possesses a basic leu- cine zipper structure at the carboxyl terminus. MafB is expressed only in monocytes and macrophages among hematopoietic cells, and it plays an important role in the ...
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Cellular Werner Phenotypes in Mice Expressing a Putative Dominant-Negative Human WRN Gene
... a dominant-negative mutant could impair K577M-WRNp may generate a multimeric complex itself the functions of other related helicases, which could and mouse WRNp may make the complex itself, and have at ...
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Dominant negative IKZF1 mutations cause a T, B, and myeloid cell combined immunodeficiency
... expressing low levels of Ikaros, showed increased myeloid precur- sors with impaired migratory potential and increased susceptibil- ity to cell death at the embryonic stage; adult mice showed a near 2-fold reduction in ...
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Dominant negative SERPING1 variants cause intracellular retention of C1 inhibitor in hereditary angioedema
... that dominant-negative effects of the disease allele are evident intracellularly and directly involved in restricting C1INH secretion leading to HAE type ...a dominant-negative effect, we ...
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Dominant negative mutations of the scavenger receptor Native receptor inactivation by expression of truncated variants
... decrease in the uptake and degradation of Ac LDL but did not decrease the degradation of beta-migrating very low density lipoprotein, which is LDL receptor-mediated. These results demonstrate that expression of truncated ...
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Inactivation of sortilin (a novel lysosomal sorting receptor) by dominant negative competition and RNA interference
... To study lysosomal trafficking pathways in living cells, several groups have taken advantage of mutant proteins and RNAi to disclose the role of proteins involved in these processes. Although both methods provide useful ...
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Susceptibility to hepatotoxicity in transgenic mice that express a dominant negative human keratin 18 mutant
... the dominant negative arg mutant human K18 develop chronic hepatitis which led us to hypothesize that some cases of human idiopathic hepatitis may be caused by mutations in human K18 ...
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Dilated cardiomyopathy in transgenic mice expressing a dominant negative CREB transcription factor in the heart
... scriptional control of the cardiac-specific a -MHC promoter. This mutant CREB protein which contains an Ala for Ser sub- stitution at aa 133 cannot be phosphorylated (and activated) and has been shown previously to ...
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Dominant negative effect of the heterozygous C104R TACI mutation in common variable immunodeficiency (CVID)
... The demonstration that WT TACI monomers preassociate prior to ligand binding is critical to understanding the molecular mecha- nisms by which heterozygous mutations in TACI that affect ligand binding can cause B cell ...
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A dominant negative provides new insights into FAK regulation and function in early embryonic morphogenesis
... FAK is a non-receptor tyrosine kinase involved in a wide variety of biological processes and crucial for embryonic development. In this manuscript, we report the generation of a new FAK dominant negative ...
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