Dried Blood Spots
Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
... ABSTRACT Human papillomavirus (HPV) vaccination elicits high-titer genotype- specific antibody responses that are associated with a reduced risk of cervical dis- ease caused by vaccine-incorporated genotypes. Our ...
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Pooled PCR testing of dried blood spots for infant HIV diagnosis is cost efficient and accurate
... age using EDTA whole blood, plasma or dried blood spots (DBS) [3], while the importance of testing infants at birth has recently been emphasised [4]. Reducing the cost of EID testing would ...
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Stability of gametocyte specific Pfs25 mRNA in dried blood spots on filter paper subjected to different storage conditions
... processing, dried blood spots (DBS) on filter paper can be ...whole blood. QT-NASBA was performed on fresh whole blood (FWB) samples as well as DBS filter paper samples stored under ...
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Evaluation of Dried Blood Spots for Human Immunodeficiency Virus Type 1 Drug Resistance Testing
... Dried blood spots (DBS) are simpler to prepare, store, and transport than plasma or serum and may represent a good alternative for drug resistance genotyping, particularly in resource-limited ...
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Long term storage limits PCR based analyses of malaria parasites in archival dried blood spots
... archived blood spots for such molecular analyses diminishes with time, most probably because of degrad- ation leading to the fragmentation of ...on blood samples that were dried on a single ...
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Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots
... using dried blood spots (DBS) routinely collected in perinatal screening ...of blood and storage conditions may be suboptimal for epigenetic ...
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Improved Sensitivity of a Dual Target HIV 1 Qualitative Test for Plasma and Dried Blood Spots
... The use of nucleic acid detection for HIV type 1 (HIV-1) detection is strongly recommended in infants <18 months of age, in whom serology is unreliable. This study evaluated the Cobas AmpliPrep/Cobas TaqMan HIV-1 ...
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Reliability of enzyme assays in dried blood spots for diagnosis of 4 lysosomal storage disorders
... Measurement of lysosomal enzymes activities in dried blood spots (DBS) on filter paper was introduced in 2001 [6] as a method with several advantages over test- ing in leukocytes/fibroblasts. First, ...
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Hyperphenylalaninemia Due to Dihydropteridine Reductase Deficiency: Diagnosis by Enzyme Assays on Dried Blood Spots
... A simple method of DHPR assay using erythrocytes or dried blood spots on filter papers is described.. The mean DHPR activity erythrocytes of con-.[r] ...
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Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging
... in dried whole blood spot samples. The collection of dried blood spots (DBS)-drops of whole blood on filter paper from a single finger prick-has recently become a popular mini- ...
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Determination of Calcineurin Inhibitors in Dried Blood Spots from Kidney Transplant Recipients
... in dried blood spots (DBS) could enable drug monitoring in transplanted patients without the necessity of having to take venous blood ...whole blood for determination of CsA and Tac in ...
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Separation of Plasma from Whole Blood by Use of the cobas Plasma Separation Card: a Compelling Alternative to Dried Blood Spots for Quantification of HIV 1 Viral Load
... response. Dried blood spots (DBSs) hold considerable logistical advantages over EDTA samples, but they more frequently misclassify virological fail- ure and have higher limits of detection ...whole ...
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The development and validation of dried blood spots for external quality assurance of syphilis serology
... finger-prick blood has been obtained to perform syphilis POC testing, DBS can be used to collect blood for EQA purposes directly ...Finger-prick blood spotted onto filter paper can be stored and ...
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Dried blood spots as a source of anti malarial antibodies for epidemiological studies
... storing blood samples which does not require that facilities for centrifugation are accessible, and which is relatively robust to irregular degrees of refrigera- tion, at least for short ...as dried ...
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Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots
... the blood samples after collection and reduce the complexity of the ...procedure. Blood spots collected on filter paper have been used for DNA amplification purposes, but reports on the feasibility ...
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Evaluation of the Aptima HIV 1 Quant Dx Assay Using Plasma and Dried Blood Spots
... The global scale-up of ART and the concomitant increased requirement for virus load monitoring represent an opportunity to build laboratory capacity in both resource-poor and resource- rich settings. Therefore, the ...
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Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta analysis
... alternative, dried blood spots (DBS) polymerase chain reaction (PCR) assays are getting more and more attention, because specimens can be collected routinely and preserved easily; and because PCR can ...
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Quantitation of Cytomegalovirus DNA Load in Dried Blood Spots Correlates Well with Plasma Viral Load
... (venipuncture), blood was obtained by finger stick by study personnel as ...resulting blood was blotted directly onto FTA-Elute cards (Whatman International ...the dried blood spots ...
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Evaluation of dried blood spots as a feasible alternative to plasma for detection, quantification and genotyping of hepatitis C virus: A pilot study
... Dried blood spots (DBS) have recently been studied as an alternative specimen to plasma for detection of antibodies to HCV as well as for detection and quantification of HCV RNA ...of blood ...
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A nested real time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
... participant, blood-smear microscopy and non-quantitative, nested PCR were per- formed on blood samples in chronological order starting from the initial enrolment visit until the first ...of blood ...
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