duplex DNA
Duplex DNA Capture
... of duplex DNA from very complex mixtures of ...of DNA out of a very heterogeneous pool of dsDNA ...of DNA retains its duplex structure and is inaccessible for binding of an ODN with a ...
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Duplex DNA Invading γ Modified Peptide Nucleic Acids Enable Rapid Identification of Bloodstream Infections in Whole Blood
... of duplex DNA-invading artificial nucleic acid oligomers that have signifi- cant advantages in kinetics, sensitivity, and specificity over DNA, RNA, and standard peptide nucleic acids; these ...
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Recognition of base J in duplex DNA by J-binding protein
... of duplex DNA required at regions flanking base J without synthesizing dif- ferent sized ...of DNA duplex rather than those due to potential varying J levels in each J-oligo ...complementary ...
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Bombyx mori Nucleopolyhedrovirus Encodes a DNA-Binding Protein Capable of Destabilizing Duplex DNA
... baculovirus DNA-binding protein, Bm- NPV DBP, poses intriguing question about the possible func- tion of this protein in virus ...bound DNA against exo- nucleases ...of DNA in an ori region provided ...
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Methods for Non Destructively Separating or Reannealing the Strands of Circular Duplex DNA Chromosomes
... Figure 6. Velocity sedimentation coefficient ( S ) vs. pH titration of the duplex replicative form (“RF”) of φ x174. The bulk solvent was 0.2 M NaCl, 0.025 M EDTA, 0.05 M NaH- CO3, i.e. , over 0.2 M salt. At pH ...
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Structural and kinetic profiling of allosteric modulation of duplex DNA induced by DNA-binding polyamide analogues
... impact DNA binding affinity for its target sequence, NMR structural analysis reveals the larger iPr-Im unit does induce more pronounced structural perturbation of the target dsDNA duplex relative to PA1, ...
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Mechanism of Degradation of Duplex DNA by the DNase Induced by Herpes Simplex Virus
... HSV-I specific DNA polymerase activity 0 and the release of acid-soluble nucleotides A were measured for calf thymus DNA which had been treated for the indicated times with A DNase I, B [r] ...
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Purification and Characterization of West Nile Virus Nucleoside Triphosphatase (NTPase)/Helicase: Evidence for Dissociation of the NTPase and Helicase Activities of the Enzyme
... Helicases are capable of enzymatically unwinding duplex DNA or RNA structures by disrupting the hydrogen bonds that keep the two strands together (18, 21). The unwinding reaction is accomplished by the ...
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RusA Holliday junction resolvase: DNA complex structure - insights into selectivity and specificity
... to duplex DNA is the first for a resolvase–DNA complex and has allowed us to construct a plausible model for the RusA–HJ complex formed during the latter stages of homologous DNA ...and ...
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Addressable and unidirectional energy transfer along a DNA three-way junction programmed by pyrrole-imidazole polyamides
... These experiments were designed to test whether PAs could pro- gramme a uni-directional FRET process along a DNA duplex and a 3WJ. Our experiments show that this is indeed the case, however one particular ...
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A structurally conserved motif in γ herpesvirus uracil DNA glycosylases elicits duplex nucleotide flipping
... into DNA necessitates a different mechanism of DNA bind- ing to that of other ...upon DNA binding which includes movement of the leucine loop approximately 3 ˚ A in the same plane as the DNA ...
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Single base variation in DNA fragment joined to a GC-clamp can be detected by temporal temperature gradient gel electrophoresis
... of DNA sequence differences. Duplex DNA fragments differing by single base substitutions can be separated by electrophoresis in Temporal Temperature gradient ...amplified DNA fragments that ...
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Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40, stump-tailed macaque virus, and SA12 virus.
... All of the heteroduplexes exhibited some base sequence homology and, at each effective temperature, the regions of duplex DNA in the heteroduplexes were measured and localized on the SV4[r] ...
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Transfection in Pneumococcus: Single-Strand Intermediates in the Formation of Infective Centers
... Transfection was reduced much more strongly than transformation in cells that were deficient in the membrane-bound endonuclease required for conversion of donor duplex DNA to intracellul[r] ...
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Ions in Water: Free Energies, Surface Effects, and Geometrical Constraints.
... [12] MacKerrell AD. In‡uence of magnesium ions on duplex DNA: structural, dynamic, and solvation properties. E¤ect of electrostatic force truncation on interfacial and transport properti[r] ...
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PubMedCentral-PMC4746636.pdf
... and DNA show a decrease in the phase and an increase in amplitude signals, respectively, relative to the mica surface, with proteins producing greater contrast than DNA on mica ...reveals DNA paths ...
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The human DNA glycosylases NEIL1 and NEIL3 excise psoralen induced DNA DNA cross links in a four stranded DNA structure
... four-stranded DNA structure (Supplementary ...four-stranded DNA replication intermediates and separate covalently linked sister ...three-stranded DNA, the complete removal of ICL adducts in both ...
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The Revitalization of the Community based Management of Acute Malnutrition Program in Haiti
... strand DNA sequence in Tris-HCl-50 mM K + buffer at pH ...and duplex DNA, ...ramps: DNA melting from 25 – 95 °C, DNA annealing for 95 – 25 °C, then melting at 25 – 95 °C, and again ...
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A Rapid, Label-free and Impedimetric DNA Sensor Based on PNA-modified Nanoporous Gold Electrode
... for DNA sensing [11]. PNA probe possesses two main advantages over DNA probe for hybridization: firstly, no electrostatic repulsion between PNA and negatively charged DNA, which make PNA can be ...
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Structure of the inverted terminal repetition of adenovirus type 2 DNA.
... Duplex DNA of 480d3ilV, has the lambdoid phage type cohesive ends of length 12 nucleotides, T7 DNA which has flush ends, duplex Ad2 EcoRI-E fragment, which has staggered ends of 4 nucleo[r] ...
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