Duplex-PCR
Comparison of an in house real time duplex PCR assay with commercial HOLOGIC® APTIMA assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urine and extra genital specimens
... Individuals at risk of contracting STIs include key-populations such as MSM, adolescents, women ≤ 24 years of age and pregnant women attending antenatal clinics, that have documented high prevalence of N. gonorrhoeae and ...
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Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex PCR Based Restriction Analysis and Direct Sequencing
... novel duplex PCR method which can differentiate Mycobacterium tuberculosis and nontuberculosis mycobacteria (NTM) strains by amplifying hsp65 DNAs of different sizes (195 and 515 bp, ...a duplex ...
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Development of a Duplex PCR Genotyping Assay for Distinguishing Clostridium perfringens Type A Isolates Carrying Chromosomal Enterotoxin (cpe) Genes from Those Carrying Plasmid Borne Enterotoxin (cpe) Genes
... Given these promising results, further testing of the duplex PCR cpe genotyping assay was performed to confirm its reli- ability. For this purpose, purified DNA was extracted from 1 additional cpe-negative ...
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Differential Identification of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNA Polymerase Gene (rpoB)
... novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used ...
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Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay
... diagnostic PCR can be used for the unambiguous identification of invasive ...the PCR mixture a second primer set, based on the ...sensitive duplex PCR assay that is specific for the detection ...
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Duplex Real Time PCR for Rapid Simultaneous Detection of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Amphibian Samples
... and duplex runs for ...of PCR inhibition accounts for this difference in detection limits between swabs and ...simplex PCR assays also tested negative in the du- plex PCR ...simplex PCR ...
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Anaplasma marginale and A phagocytophilum in cattle in Tunisia
... conventional PCR for the detection of ...a duplex format. The optimized duplex PCR was able to specifically detect ...the duplex PCR entirely corresponded with the results ...
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Estimating trematode prevalence in snail hosts using a single step duplex PCR: how badly does cercarial shedding underestimate infection rates?
... of PCR over cercarial ...multiplex PCR for identification and discrimination of Caliophoron daubneyi and Fasciola hepatica in the snail Galba truncatula , but they did not find natural double infections, ...
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Detection of Methicillin Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay
... third-generation PCR technology: the droplet digital PCR ...of PCR has made it the method of choice for the rapid detection of MRSA in a clinical setting (17, ...A duplex PCR assay was ...
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Characterization of Salmonella enterica Serovar Typhimurium and Monophasic Salmonella Serovar 1,4,[5],12:i: Isolates in Thailand
... were duplex PCR positive was ...using PCR to amplify the gene encoding flagella, and these researchers found that a number of serovar 1,4,[5],12:i:- isolates were pos- itively amplified for this gene ...
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Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan
... effective duplex PCR assay for the detection of adenovirus and chicken anaemia viruses in poultry birds which is capable to detect the prevalent viral types in ...
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Definitive Differentiation between Single and Mixed Mycobacterial Infections in Red Deer (Cervus elaphus) by a Combination of Duplex Amplification of p34 and f57 Sequences and Hpy188I Enzymatic Restriction of Duplex Amplicons
... of duplex us-p34–f57 amplicons generated specific banding patterns for ...the duplex PCR in the first step of the PCR-RFLP analysis, so the hypothetical mixed ...
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Development of a Duplex Real Time PCR Method for the Pharmaceutical Rapid Microbial Detection of Staphylococcus aureus and Pseudomonas aeruginosa
... real-time PCR (RT-PCR) allows turnaround time and eh risk of contamination with PCR products to be reduced compared to the conventional PCR ...specific duplex real-time PCR assay ...
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A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
... Pathogens and construction of recombinant plasmids DNA was first extracted from MS samples as described previously [17], and total RNA was extracted from ARV samples using TRIzol reagent (Life Technologies, Carlsbad, CA, ...
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Switching Gears for an Influenza Pandemic: Validation of a Duplex Reverse Transcriptase PCR Assay for Simultaneous Detection and Confirmatory Identification of Pandemic (H1N1) 2009 Influenza Virus
... the duplex RT- PCR assay was highly sensitive at identifying swH1 (Table ...the duplex RT-PCR ...the duplex RT-PCR assay greatly influenced the abil- ity of the assay to detect ...
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A hybrid half duplex/full duplex transmission scheme in relay aided cellular networks
... Some researchers also considered hybrid HD/FD transmission schemes, which combine the advantages of both HD and FD. In [23], an optimal transmission scheduling scheme for a hybrid HD/FD relaying system was proposed, ...
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Opportunistic half duplex/full duplex relaying mode selection criterion in cognitive relay networks
... single duplex relaying system. A hybrid duplex scheme was proposed in a random-access wireless network and heterogeneous wireless networks [25, 26], which could get a high ...
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Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay
... genotypic PCR CPS typing ...a PCR typing scheme that was de- veloped to target the serotype-determining region of the cps locus for Ia, Ib, and II to ...real-time PCR typing assay which successfully ...
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Identification of Rifampin Resistant Mycobacterium tuberculosis Strains by Hybridization, PCR, and Ligase Detection Reaction on Oligonucleotide Microchips
... ization and on-chip PCR and in denaturing polyacrylamide gel electrophoresis for on-chip LDR. To immobilize oligonucleotides in gel pads or to attach the fluorescent label Texas Red (Molecular Probes, Inc., ...
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Thalassemia intermedia in HbH CS disease with compound heterozygosity for beta thalassemia: challenges in hemoglobin analysis and clinical diagnosis
... the PCR control where no DNA was added. Using a duplex-PCR to detect the SEA deletion, patient and mother’s DNA amplified the 730 bp SEA- deletion-specific fragment and 136 bp normal sequence (gel 1, ...
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