duplex PCR
Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex PCR Based Restriction Analysis and Direct Sequencing
... Multiplex PCR methods targeting several genes (4, 13, 15, 22) and duplex PCR targeting a single gene, the RNA poly- merase gene (11), have been developed for the differential identification of ...
8
Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan
... losses. Duplex PCR assay devel- oped in this study has 100% specificity and high sensi- tivity to detect AAV and CAV infections in poultry birds and is a major improvement in poultry diagnostics of Pakistan ...
5
Comparison of duplex PCR and phenotypic analysis in differentiating Candida dubliniensis from Candida albicans from oral samples
... cumbersome. Duplex PCR analyses for ...of duplex PCR was then compared with phenotypic identification using a standard battery of phenotypic ...by duplex PCR as ...dubliniensis. ...
6
Differential Identification of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria by Duplex PCR Assay Using the RNA Polymerase Gene (rpoB)
... novel duplex PCR method that can amplify the 235- and 136-bp rpoB DNAs of Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM), respectively, with two different sets of primers was used ...
5
Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences
... related PCR assay based on kinetoplast DNA was highly sen- FIG. 5. Duplex PCR in different samples visualized in ethidium bromide-stained 3% agarose ...
5
Chromocult Coliform agar and duplex PCR assays as methodologies for tracking Escherichia coli K12 in industrial biotechnological processes
... the duplex PCR assay for Escherichia coli K12 in waste and intermediate products of an industrial biotechnological ...0.733). Duplex PCR analysis was shown to be able to differentiate a ...
7
Nested Duplex PCR To Detect Bordetella pertussis and Bordetella parapertussis and Its Application in Diagnosis of Pertussis in Nonmetropolitan Southeast Queensland, Australia
... nested duplex PCR and ...the PCR assay 100% sensitivity; how- ever, a large number of PCR-positive–culture-negative speci- mens were ...positive PCR result. A ...
5
Prevalence and Evaluation of Toxin Genes among Uropathogenic Escherichia coli Clinical Isolates by Duplex PCR
... and pap by using molecular methods ex. Duplex PCR may be informative for the best UTI treatment. Some other virulence genes have been assessed among UPEC pathotype strains. The results elucidated a relation ...
6
New Sensitive One Step Real Time Duplex PCR Method for Group A and B HIV 2 RNA Load
... The aim of the present work was to develop a new sensitive method for quantifying HIV-2 RNA, particularly that of epidemic groups A and B. It is based on a one-step real-time duplex PCR method using the ...
6
Development and Evaluation of a Single Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)
... the duplex PCR using reference Fasciola ...the duplex PCR ...the PCR amplicons yielded were good quality even from DNA template extracted from squeezed ...eggs. PCR products from ...
7
Duplex PCR for Differential Identification of Mycobacterium bovis, M avium, and M avium subsp paratuberculosis in Formalin Fixed Paraffin Embedded Tissues from Cattle
... A duplex diagnostic PCR strategy based on coamplification of nonhomolo- gous us-p34 and species-specific f57 sequences was therefore ...developed. Duplex PCR yielded three different patterns, ...
7
Estimating trematode prevalence in snail hosts using a single step duplex PCR: how badly does cercarial shedding underestimate infection rates?
... of PCR-based techniques. We thus devel- oped a duplex PCR assay for two host-parasite systems, capable of specifically amplifying differentially sized seg- ments of the internal transcribed spacer ...
11
Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay
... diagnostic PCR can be used for the unambiguous identification of invasive ...the PCR mixture a second primer set, based on the ...sensitive duplex PCR assay that is specific for the detection ...
5
Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... real-time PCR method to detect HIV type 1 (HIV-1) DNA in dried blood ...This duplex PCR assay may be useful in identi- fying HIV-1-infected persons, particularly infants born to seropositive mothers ...
7
Accurate Detection of Methicillin Resistant Staphylococcus aureus in Mixtures by Use of Single Bacterium Duplex Droplet Digital PCR
... used duplex PCR methods are not able to differentiate MRSA from coex- isting methicillin-susceptible ...digital PCR (ddPCR) with the chimeric lysin ClyH for cell ...the duplex- positive ...
10
Simultaneous Detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by Use of Molecular Beacons in a Duplex Real Time PCR
... real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with ...
5
Detection of Methicillin Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay
... third-generation PCR technology: the droplet digital PCR ...of PCR has made it the method of choice for the rapid detection of MRSA in a clinical setting (17, ...A duplex PCR assay was ...
7
Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT-PCR
... For duplex PCR two sets of universal primers were used in one ...The duplex-RT-PCR was performed in a 25 µL total reaction volume with ...10x PCR Buffer, 1 mM MgCl 2 , 50 ...
5
Duplex Real Time PCR for Rapid Simultaneous Detection of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Amphibian Samples
... and duplex runs for ...of PCR inhibition accounts for this difference in detection limits between swabs and ...simplex PCR assays also tested negative in the du- plex PCR ...simplex PCR ...
6
The establishment of a duplex real-time PCR assay for rapid and simultaneous detection of blaNDM and blaKPC genes in bacteria
... conventional PCR and sequencing. The ex- cellent performance of duplex PCR makes it an important tool for guiding the appropriate choice of antimicrobial therapy and helping limit spread of the ...
5