Duplex Real-Time PCR
Development of a Duplex Real Time PCR Method for the Pharmaceutical Rapid Microbial Detection of Staphylococcus aureus and Pseudomonas aeruginosa
... of real-time PCR (RT-PCR) allows turnaround time and eh risk of contamination with PCR products to be reduced compared to the conventional PCR ...specific duplex ...
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A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
... timized duplex real-time PCR assay to detect ARV and ...the duplex real-time PCR assay was determined using serial 10-fold dilutions (10 1 -10 8 copies/μl) of ...
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Development of a panel of seven duplex real-time PCR assays for detecting 13 streptococcal superantigens
... the duplex system for the detection of streptococcal SAgs were analyzed and compared to the results obtained from the conventional PCR ...the duplex system was at least a 10 -3 di- lution from the ...
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Simultaneous Detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by Use of Molecular Beacons in a Duplex Real Time PCR
... A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons ...
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Duplex Real Time PCR Assay for Detection of Streptococcus pneumoniae in Clinical Samples and Determination of Penicillin Susceptibility
... (14). Duplex real-time ...and PCR-grade water up to a total volume of 40 ...pbp2b PCR-RFLP ...gene PCR, which includes a control for PCR inhibition ...
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Duplex Real Time PCR for Rapid Simultaneous Detection of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Amphibian Samples
... possible PCR inhibition (9), and were stored at ⫺ 20°C until further ...and duplex real-time PCR assays, for comparison of the variability in C q values between the simplex and ...
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The establishment of a duplex real-time PCR assay for rapid and simultaneous detection of blaNDM and blaKPC genes in bacteria
... the duplex real-time PCR assay were specifically designed to amplify all alleles of each gene family described above using Beacon Designer software (Premier Biosoft, Palo Alto, CA, USA) (Table ...
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A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... novel duplex TaqMan real-time PCR assay for the simultaneous detection of MG and ...This duplex real-time PCR assay incorporates TaqMan (FAM/NED) labelled minor ...
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Development and validation of a duplex real time PCR assay for the diagnosis of equine piroplasmosis
... performance, quantitative ability and a lower risk of carry- over contamination due to the absence of a need for post- amplification manipulation of the products [31]. Genomic sites targeted by described qPCR assays for ...
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Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... the real-time assay, the numbers of HIV copies used in the ...single real-time run to determine the intrarun variability (see also Table ...the duplex assay. (A) Real-time ...
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Evaluation of the LightCycler Methicillin Resistant Staphylococcus aureus (MRSA) Advanced Test for Detection of MRSA Nasal Colonization
... Culture methods. The swab heads were streaked onto ChromID MRSA agar (bioMérieux, France) directly before they were cut and added to the sample preparation buffers, which were used for DNA extraction for both LightCycler ...
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Identification of Group B Streptococcus Capsule Type by Use of a Dual Phenotypic/Genotypic Assay
... by PCR. Several molecular typing-based PCR assays have been developed (36, 38, 40, 41, ...operon. PCR assays are an attractive approach to GBS CPS typing because of their high discriminatory power ...
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Comparison of a Duplex Quantitative Real Time PCR Assay and the COBAS Amplicor CMV Monitor Test for Detection of Cytomegalovirus
... The target regions in the CMV AD169 genome (accession no. X17403) were pol, at nucleotides (nt) 78197 to 78262, and gB, at nt 81855 to 81916. Primers and probes were designed as previously described by Yun et al. (23, ...
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Detection of Anaplasma marginale and A phagocytophilum in Bovine Peripheral Blood Samples by Duplex Real Time Reverse Transcriptase PCR Assay
... Ratio of 16S rRNA to 16S DNA of A. marginale. The number of 16S rRNA molecules present in Anaplasma species is unknown. This results in uncertainty in determining the minimum detection sensitivity of the assay in a ...
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New Sensitive One Step Real Time Duplex PCR Method for Group A and B HIV 2 RNA Load
... a duplex method capable of simultaneously amplifying the LTR and gag regions, in order to retain the benefits of each previous test and to improve the detec- tion of group B viruses by reducing the risk of ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... a duplex high-resolution melt and ...mismatch real-time ...first time that such a large panel of strains has been ana- lyzed, and we also report the first isolation of ...
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... one-step duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different ...
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Duplex quantitative real time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples
... Numerous PCR methods for the de- tection and identification of ...Accordingly, real-time PCR has the potential to cir- cumvent the drawbacks of endpoint ...Moreover, real-time ...
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Comparison of an in house real time duplex PCR assay with commercial HOLOGIC® APTIMA assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urine and extra genital specimens
... in-house duplex PCR ...in-house duplex PCR assay, whereas the APTIMA assay systems require double that volume, ...ventional PCR assay approach, resulting in higher amplification levels, ...
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Development of a duplex SYBR Green I based quantitative real time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
... quantitative PCR was also applied for detection and quantification of other viruses [23, ...the PCR products [1, 32]. Here, we developed a duplex SYBR Green-I based quantitative ...
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