E. coli DNA, polymerase I
The UmuC subunit of the E coli DNA polymerase V shows a unique interaction with the β clamp processivity factor
... of polymerase interaction with the β-clamp is complex, particularly when considering the multimeric Pol III and Pol ...of DNA by the clamp differentially influences polymerase ...of DNA ...
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Antimutator mutations in the alpha subunit of Escherichia coli DNA polymerase III: identification of the responsible mutations and alignment with other DNA polymerases.
... The dnaE gene of Escherichia coli encodes the DNA polymerase ( a subunit) of the main replicative enzyme, DNA polymerase I l l holoenzyme.. We have previously identified th[r] ...
6
Reversion of a Moloney murine leukemia virus RNase H mutant at a second site restores enzyme function and infectivity.
... double-stranded DNA form (1a, ...a DNA polymerase activity, which synthesizes DNA from either RNA or DNA templates, and an RNase H activity that degrades genomic RNA in RNA- DNA ...
5
Genetic Functions Promoting Homologous Recombination in Escherichia coli: A Study of Inversions in Phage Λ
... In addition to RecA protein and RecBCD enzyme, inversion by the RecBC pathway required single-stranded DNA binding protein, DNA gyrase, DNA polymerase I and DNA li[r] ...
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DNA sequence effects on single base deletions arising during DNA polymerization in vitro by Escherichia coli Klenow fragment polymerase.
... Most single base deletions detected after DNA polymerization in vitro directed by either Escherichia coli DNA polymerase I or its Klenow fragment are opposite Pu in the t[r] ...
11
Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid
... RNA polymerase gene in the host chromosome at the lacUV5 promoter, and expression of recombinant protein at the T7 promoter are not controlled in BL21 ...RNA polymerase, despite the T7 lysozyme encoded in ...
9
Spontaneous mutation in Escherichia coli containing the dnaE911 DNA polymerase antimutator allele.
... These new strains had muta- tions in the dnaE gene (polymerase subunit) of DNA polymerase I11 holoenzyme, and conferred an antimu- tator phenotype in a mismatch-repair-d[r] ...
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Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1.
... Mutational analysis of the DNA polymerase and ribonuclease H activities of human immunodeficiency virus type 2 reverse transcriptase expressed in Escherichia coli.. Reconstitution in vit[r] ...
9
I protein: bacteriophage T7-coded inhibitor of Escherichia coli RNA polymerase.
... Although, as will be described in the following section, I protein does not prevent the binding of core polymerase with T7 DNA, our holoenzyme preparation used in this experiment was sat[r] ...
15
Differentiation of Avian Pathogenic Escherichia coli Strains from Broiler Chickens by Multiplex Polymerase Chain Reaction (PCR) and Random Amplified Polymorphic (RAPD) DNA
... Escherichia coli (E. coli) is one of the most common and important avian bacterial pathogens and infections caused by ...E. coli are responsible for significant economic losses to the ...
10
Efficacy of Andrographis paniculata against extended spectrum β lactamase (ESBL) producing E coli
... of E. coli together with in-silico studies. E. coli strains were also subjected to DNA isolation and gene expression analysis through polymerase chain reac- tion (PCR) to study ...
9
Involvement of the Reparative DNA Polymerase Pol X of African Swine Fever Virus in the Maintenance of Viral Genome Stability In Vivo
... The Pol X deletion mutant virus was obtained by insertion of the gusA gene of E. coli coding for  -glucuronidase into the O174L open reading frame (ORF) of the ASFV strain BA71V. For this, left and right ...
8
DNA polymerase II of Escherichia coli in the bypass of abasic sites in vivo.
... Mutagenesis accompanied SOS bypass of abasic sites when the original occupant had been cytosine but not when it had been thymine; the quantitative difference is show[r] ...
10
The Roles of Klenow Processing and Flap Processing Activities of DNA Polymerase I in Chromosome Instability in Escherichia coli K12 Strains
... of E. coli linkage map tonB-trp region and the loca- tions of primers for PCR ampli- fication and sequencing (Yama- mura et ...bp DNA sequences in which 1 is the first G of the GATC BamHI site are ...
11
Genetic analysis of gene 1.2 of bacteriophage T7: isolation of a mutant of Escherichia coli unable to support the growth of T7 gene 1.2 mutants.
... The products of at least six genes of phage T7 are essential for T7 DNA replication: gene 1 RNA polymerase, gene 2 inhibitor of Escherichia coli RNA polymerase, gene 3 endonuclease, gene[r] ...
9
Lysogenization of Escherichia coli by bacteriophage Lambda: complementary activity of the host's DNA polymerase I and ligase and bacteriophage replication proteins Q and P.
... Lysogenization ofEscherichia coli by Bacteriophage Lambda: Complementary Activity of the Host's DNA Polymerase I and Ligase and Bacteriophage Replication Proteins 0 and P USHARANJAN RAY'[r] ...
7
Involvement of Escherichia coli K-12 DNA polymerase I in the growth of bacteriophage Mu.
... Frequency of Mu-mediated transposition of chromosomal genes after infection with MupApl Transposition frequencya Donor strain pro+ thr+ AT3820 polAl, F::TnlO 6.2 x 10-7 3.1 x 10-7 AT3821[r] ...
8
Repair of single- and multiple-substitution mismatches during recombination in Streptococcus pneumoniae.
... RADMAN, 1986 Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system. KNILL-JONES, 1981 DNA polymerase accura[r] ...
8
Subunits of DNA polymerase III holoenzyme of Escherichia coli
... Since the holoenzyme contains all of the subunits core, y, o, t, 8' and~ found to be essential for processive replication O'Donnell, 1987; Studwell and O'Donnell, 1990, it may also have [r] ...
260
Structural basis for proofreading during replication of the Escherichia coli chromosome
... Table of contents Chapter 1 Xll Structures and Functions of DNA Polymerases l 1.1 Escherichia coli DNA replication 2 1.2 DNA polymerase III holoenzyme 1.2.1 Fidelity, processivity and ef[r] ...
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