E1 and E2
CK2 Phosphorylation Inactivates DNA Binding by the Papillomavirus E1 and E2 Proteins
... in E1 and E2 on DNA replication and morphological ...the E1 and E2 proteins could be altered by CK2 phosphory- ...in E2 showed a greatly increased level of replication compared to that ...
12
Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection
... proteins; E1 and E2 of envelope, and nucleocapsid ...protein E1 and E2 are components of spikes, which composed of triplets of heterodimer of E1 and E2 glycoproteins, and cover ...
8
Binding of the E1 and E2 proteins to the origin of replication of bovine papillomavirus.
... the E1- containing complexes that can form on the BPV ori ...observed, E1 alone gave rise to a complex that showed lower electrophoretic mobility than the complex formed in the presence of E2, and ...
10
Negative regulation of the bovine papillomavirus E5, E6, and E7 oncogenes by the viral E1 and E2 genes.
... (i) E2 repressors suppress transformation by the E6 and E7 oncogenes, and (ii) E1 and the E2 transactivator suppress transformation by the E6, E7, and E5 ...the E2 repressors function to ...
8
Functional interactions between papillomavirus E1 and E2 proteins.
... the E1 and E2 proteins were expressed by using the pET system ...of E1 will be presented elsewhere (43). The E2 proteins in this study were purified using DNA affinity chromatography as ...
11
Effects of Mutations in the Rubella Virus E1 Glycoprotein on E1-E2 Interaction and Membrane Fusion Activity
... and E2 [42 to 47 kDa]), lo- cated on the virion surface (32), that exist as heterodimers in the viral spike complexes (3, ...49). E1, the major target antigen of RV, contains virus-neutralizing and ...
9
Lectin-Mediated Retention of p62 Facilitates p62-E1 Heterodimerization in Endoplasmic Reticulum of Semliki Forest Virus-Infected Cells
... whether E2* was produced in CST-treated cells that had been infected with wt virus and pulse-labeled, by immunoprecipitation with a monoclonal anti-E2 ...the E2* of an SFV p62 vector-transfected cell ...
7
Evaluating Replication-Defective Vesicular Stomatitis Virus as a Vaccine Vehicle
... VSV-expressed E1 and E2 coimmunoprecipitate as heterodimers in infected cell lysates ...pressed E2 products interacted with conformationally sensitive anti-E2 H33 and nonconformationally ...
16
The Carboxyl-Terminal Region of the Human Papillomavirus Type 16 E1 Protein Determines E2 Protein Specificity during DNA Replication
... hybrid E1 protein genes were constructed (Fig. 2). Each hybrid E1 gene encodes a protein identical in length (649 amino acids) to that encoded by the wild-type HPV-11 or HPV-16 gene or a modified HPV-11 ...
6
Chikungunya Viruses That Escape Monoclonal Antibody Therapy Are Clinically Attenuated, Stable, and Not Purified in Mosquitoes
... the E1 and E2 genes during the persistence stage failed to reveal rever- ...(CHIKV-E1-K61T E2-D59N) showed enhanced fitness as the mutant but not WT virus sequence was detected in tissue ...
14
Kinase Activity of Fibroblast Growth Factor Receptor 3 Regulates Activity of the Papillomavirus E2 Protein
... on E2-stimulated viral DNA replication was subsequently evaluated using C33A cells transfected with BPV-1 E1 and E2 expression vectors, along with a BPV-1 luciferase-linked reporter in the presence ...
13
Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
... block E2 expression or to antagonize E2 ...block E2 mRNA translation and E2-mediated transcriptional trans-activation in cultured cells (7, ...papillomavirus E2-binding site, thereby ...
5
Hepatitis C Virus Glycoproteins Interact with DC-SIGN and DC-SIGNR
... (HCV) E2 glycoprotein and retrovirus pseudotypes expressing chimeric forms of both HCV E1 and E2 glycoproteins bound efficiently to DC-SIGN and DC-SIGNR expressed on cell lines and primary human ...
11
Hepatitis C Virus NS2 Coordinates Virus Particle Assembly through Physical Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes
... viral E1-E2 glycoprotein and NS3-NS4A enzyme ...with E1, E2, and NS3 but did not stably interact with viral core or NS5A ...containing E2, NS2, and ...via E2, and the pattern of ...
12
Substitution of Specific Cysteine Residues in the E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Affects Formation of E1-E2 Heterodimers and Alters Virulence in Swine
... BICv E1 yielded viable mutant ...of E1-E2 heterodimers in infected cells or virus infectivity either in vitro or in vivo, suggesting that more than one disulfide link may contribute to the formation ...
9
Cell Fusion Activity of Hepatitis C Virus Envelope Proteins
... the E1 and E2 proteins consistently exhibited higher fusion activity than those express- ing either of the chimeric envelope proteins and parental CHO ...the E1 and E2 proteins are required ...
9
Human Papillomavirus Type 16 Status in Cervical Carcinoma Cell DNA Assayed by Multiplex PCR
... genes: E1, E2, E6, and L1 (the latter is included as a known marker of HPV ...the E1/E2 PCR product ratio for different histological types of cancer and for different stages of cancer ...of ...
5
p53 Protein Is a Suppressor of Papillomavirus DNA Amplificational Replication
... proteins E1 and E2, which determine the efficiency of initiation of ...proteins E1 and/or E2 have been shown to repress the promoter which is closest to the replication origin and directs E6 ...
10
Characterization of the DNA-Binding Domain of the Bovine Papillomavirus Replication Initiator E1
... between E1 and T antigen is the re- quirement for E2 for in vivo ...of E2 as an auxiliary factor in DNA replication is to interact with E1 to form the E1E2-ori ...of E1 with ori melting ...
10
Multigene Tracking of Hepatitis C Virus Quasispecies after Liver Transplantation: Correlation of Genetic Diversification in the Envelope Region with Asymptomatic or Mild Disease Patterns
... the E1/E2 and NS2 regions and show smaller differences in regions such as E1-59 (upper panel), and NS4-59 (lower ...the E1/E2, NS2, NS4-59, and NS4-39 ...
8