E3 ligase
Differential Domain Architecture Directs Nedd4 Family E3 Ligase Function.
... Protein expression and purification - Nedd4 family member E3 ligase constructs were prepared using PCR amplification to insert restriction sites in cDNA encoding specified variants. cDNA constructs used: ...
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HIV 1 Tat Recruits HDM2 E3 Ligase To Target IRF 1 for Ubiquitination and Proteasomal Degradation
... Cell culture and reagents. J-Lat Tat-GFP cells (clone A2/A72) from Eric Verdin (45) was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. Jurkat, Jurkat clone A2/A72, and HEK293 cells were ...
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The ubiquitin E3 ligase activity of BRCA1 and its biological functions
... BRCA1 supercomplex formation in response to DNA damage. BRCA1 acts in many cellular supercomplexes and exe- cutes distinct roles. BARD1 stoichiometrically interacts with BRCA1 and exists as a RING heterodimer in all the ...
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MDM2 is a novel E3 ligase for HIV 1 Vif
... novel E3 ligase for HIV-1 ...an E3 ligase for Vif to induce its polyubiquitina- tion and proteasomal ...Several E3 ligases including Cul5 [17], Nedd4, and AIP4 [18], have been reported ...
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The ORF3 Protein of Porcine Circovirus Type 2 Interacts with Porcine Ubiquitin E3 Ligase Pirh2 and Facilitates p53 Expression in Viral Infection
... The E3 en- zymes can also be autoubiquitinated in cells, targeting them- selves for proteasomal destruction through their RING motif (10, ...ubiquitin E3 ligase hPirh2 by preventing its ...
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The E3 ligase Cdh1 anaphase promoting complex operates upstream of the E3 ligase Smurf1 in the control of axon growth
... Aside from being regulated by specific GAPs and GEFs, RhoA has also been identified as a substrate of the E3 ubiquitin ligase Smurf1 (Wang et al., 2003). Smurf1 polyubiquitylates RhoA and targets it for ...
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Oxidation of the cysteine-rich regions of parkin perturbs its E3 ligase activity and contributes to protein aggregation
... Using complementary MS strategies ─ high-resolution Q-TOF MS and high-throughput ion trap MS/MS ─ we mapped the PTMs of parkin under conditions of oxidative stress and encountered sulfination/sulfonation of specific ...
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Inhibitors of ubiquitin E3 ligase as potential new antimalarial drug leads
... Eight E3 ligase inhibitors namely, thalidomide, proTAME, NSC 66811, Nutlin 3, HLI 373, JNJ 26854165, SMER 3 and NSC 146109 were used in the ...an E3 ubiquitin ligase complex with damaged ...
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Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth
... The membrane-associated RING-CH (MARCH) proteins belong to the RING finger protein family of E3 ubiquitin ligases, consisting of 11 members in mammals. MARCH proteins have numerous cellular functions, which ...
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E3 ubiquitin ligases and their control of T cell autoreactivity
... the E3 ligases GRAIL, Cbl-b, Itch, and NEDD4 ubiquitinate and chaperone critical proximal signaling molecules into an endocytic pathway and direct them away from the immunological synapse and into a lyso- somal ...
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Comparing human–Salmonella with plant–Salmonella protein–protein interaction predictions
... HECT-like E3 ligase, which has been shown to interact with the E2 UbcH7, is known to induce polymorphonuclear neu- trophil migration, a process attributed to its Ub ligase ...host E3 ...
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Lipolytic inhibitor G0S2 modulates glioma stem-like cell radiation response
... by E3 ubiquitin ligase RNF168 in DNA dam- age [37, ...its E3 ligase activity, acceler- ates its proteolysis, and impairs its function in DNA damage response ...inhibits E3 ligase ...
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Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets
... Furthermore, we examined whether binding of wild- type spartin to AIP4 in vitro also results in increased self-ubiquitination of AIP4. We investigated the levels of self-ubiquitination of the glutathione S-transferase ...
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Latency-Associated Nuclear Antigen E3 Ubiquitin Ligase Activity Impacts Gammaherpesvirus-Driven Germinal Center B Cell Proliferation
... In addition to exerting E3 ligase activity, gammaherpesvirus LANA proteins assume a critical role for virus persistence and, hence, associated pathologies. Gammaherpesviruses infect a vari- ety of mammalian ...
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Porcine MKRN1 Modulates the Replication and Pathogenesis of Porcine Circovirus Type 2 by Inducing Capsid Protein Ubiquitination and Degradation
... that E3 ligase-mediated ubiquitin is crucial for viral degradation, such as in the process of Japanese encephalitis virus (JEV), HIV, and West Nile virus (WNV) infections (20, 35, ...an E3 ubiquitin ...
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DNA damage-induced ubiquitylation:emerging regulators enforce protective mechanisms
... and E3 ubiquitin ligases have been previously implicated in p53 regulation or DNA repair processes [5, ...an E3 ligase also proposed to control the Notch signalling pathway ...
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Enterovirus 71 induces degradation of TRIM38, a potential E3 ubiquitin ligase
... Analysis by Map Viewer software on the NCBI website showed that the TRIM38 gene is located on human chromosome 6p21.3, and clustered with other TRIM genes including TRIM10, TRIM15, TRIM26, TRIM27, TRIM31, TRIM39, and ...
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The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells
... its E3 ligase activity, the RNF20/RNF40 com- plex was shown to monoubiquitinate histone H2B at ly- sine 120 ...the E3 ligases mediating the monoubiquitination of H2B also have a tumor suppressive ...
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Hippo Stabilises Its Adaptor Salvador by Antagonising the HECT Ubiquitin Ligase Herc4
... Sav E3 ligase (Fig ...conserved E3 ligase domain ...Herc E3 ligases, which in humans comprises 4 members, Herc3-6 ...Herc E3 ligases, Herc2 and ...
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IRF3 Inhibition by Rotavirus NSP1 Is Host Cell and Virus Strain Dependent but Independent of NSP1 Proteasomal Degradation
... in E3 ligases, it is attractive to speculate that the NSP1 RING finger may encode a similar function and thus determines IRF3 deg- radation ...known E3 ligase function (44), whereas pestivirus N pro ...
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