E5 oncoprotein
The Human Papillomavirus Type 16 E5 Oncoprotein Inhibits Epidermal Growth Factor Trafficking Independently of Endosome Acidification
... 16 E5 oncoprotein (16E5) enhances acute, ligand-dependent activation of the epidermal growth factor receptor (EGFR) and concomitantly alkalinizes endosomes, presumably by binding to the 16-kDa “c” subunit ...
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hrHPV E5 oncoprotein: immune evasion and related immunotherapies
... Recently, E5 was proposed to be classified as a viro- porin, a channel protein able to modulate ion homeosta- sis, vesicle trafficking, virion production and viral genome entry ...cells, E5 ...
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The Human Papillomavirus Type 16 E5 Oncoprotein Translocates Calpactin I to the Perinuclear Region
... (HPV-16) E5 oncoprotein is embedded in membranes of the endoplasmic reticulum and nuclear envelope with its C terminus exposed to the ...activities, E5 cooperates with the HPV E6 oncoprotein ...
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A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase.
... Coprecipitation of E5-16K protein complex and E5 dimer formation in cells expressing E5 epitope proteins containing amino acid substitutions within the middle hydrophobic region.. A Extr[r] ...
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High-Risk Human Papillomavirus E5 Oncoprotein Displays Channel-Forming Activity Sensitive to Small-Molecule Inhibitors
... the E5 channel will allow the release of molecules with a Stokes’ radius of ...the E5 channel is equal to or greater than ...that E5 forms a pore with a lumi- nal diameter of ⬃ 1 to 2 nm and our in ...
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The E5 oncoprotein of human papillomavirus type 16 transforms fibroblasts and effects the downregulation of the epidermal growth factor receptor in keratinocytes.
... Kinetic studies with [125IEGF showed that the ligand underwent normal internalization and degradation in both HPV-16 ES-expressing and control keratinocytes, but in ES-expressing cells, [r] ...
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Multiple Transmembrane Amino Acid Requirements Suggest a Highly Specific Interaction between the Bovine Papillomavirus E5 Oncoprotein and the Platelet-Derived Growth Factor Beta Receptor
... the E5 protein, we constructed a variety of additional receptor chimeras and mu- tants, which are depicted in ...the E5 protein after stable coexpression with E5 in Ba/F3 cells, a murine ...
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Quantitative Measurement of Human Papillomavirus Type 16 E5 Oncoprotein Levels in Epithelial Cell Lines by Mass Spectrometry
... E4, E5, E6, and E7 early proteins ...of E5 and E1 E4 transcripts relative to GAPDH in HPV-16-negative (C33A) and HPV-16-positive (SiHa and Caski) cervical cancer cell ...
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E5 oncoprotein transmembrane mutants dissociate fibroblast transforming activity from 16-kilodalton protein binding and platelet-derived growth factor receptor binding and phosphorylation.
... of E5 mutants with regard to 16K protein association and cellular transformation: (i) those that bound 16K protein and were transformation defec- tive, (ii) those that did not associate with 16K protein but were ...
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Transformation-specific interaction of the bovine papillomavirus E5 oncoprotein with the platelet-derived growth factor receptor transmembrane domain and the epidermal growth factor receptor cytoplasmic domain.
... Consistent with the biochemical and biological activation data, coimmunoprecipitation studies demonstrated that ES formed a complex with any chimera that contained a PDGF-R transmembrane[r] ...
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Membrane Orientation of the Human Papillomavirus Type 16 E5 Oncoprotein
... HPV-16 E5 protein, which is epitope tagged at both its N and C termini, retains biological ...indicated E5 constructs to cooperate with HPV-16 E6 in the induction of koilocytosis are ...HPV-16 E5- ...
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The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes.
... while E5-expressing cells (mass culture pLXSN16E5 and clones pKV16E5, pAT16, and K1/16) failed to degrade internalized EGFR up to 3 h after EGF stimulation and exhibited recycling of receptors to the cell surface ...
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HPV16 E5 deregulates the autophagic process in human keratinocytes
... The E5 oncoprotein of human papillomavirus type 16 (HPV16 E5) perturbs epithelial homeostasis down-regulating the expression of the keratinocyte growth factor receptor (KGFR/FGFR2b), whose signaling ...
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Oncoprotein metastasis and its suppression revisited
... cer process may begin very early, specifically at the level of the DNA structure in (morphologically) normal cells adjacent to primary tumors [10]. Furthermore, it was concluded that certain post-translational events ...
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Cell recognition and pattern formation in the developing nervous system
... A,B,C Anti-TOPDV binding to dorsal, middle and ventral E5 retina, respectively; D,E,F anti-TOPDV binding to dorsal, middle and ventral E5 optic tectum, respectively; G anti-TOPDV in E18 [r] ...
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Crystallography in the Spaces E2, E3, E4, E5 N0II Isomorphism Classes and Study of Five Crystal Families of Space E5
... Crystallographic Point Groups of the Two Decadic-al Crystal Sub-Families All the WPV point group symbols of these two families contain groups [5] or 5 family N˚XXIVa and group [1[r] ...
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Supernumerary Molars and Wisdom Tooth Shape Alterations in Patients with Neurofibromatosis Type 1
... Supernumerary Molars and Wisdom Tooth Shape Alterations in Patients with Neurofibromatosis Type 1 J Oral Maxillofac Res 2017;8(4):e5.. URL: http://www.ejomr.org/JOMR/archives/2017/4/e5/[r] ...
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The human papillomavirus type 6 and 16 E5 proteins are membrane-associated proteins which associate with the 16-kilodalton pore-forming protein.
... Association of the HPV-6 E5-AU1 and HPV-16 E5-RNGS fusion proteins with the 16-kDa pore-forming component of the vacuolar ATPase.. The 16-kDa protein is a membrane component of the vacuo[r] ...
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Endoplasmic Reticulum-Localized Human Papillomavirus Type 16 E5 Protein Alters Endosomal pH but Not trans-Golgi pH
... HPV E5 did indeed alkalinize endosomes ...HPV-16 E5 pro- tein, or the ⫺ 10 deletion E5 mutant protein were transfected with the cellubrevin-GFP pHluorin ...full-length E5 protein and two ...
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The Canine Papillomavirus E5 Protein Signals from the Endoplasmic Reticulum
... epitope-tagged E5 protein migrated as a 5-kDa protein, consistent with its predicted amino acid ...While E5 tagged at its N terminus was detectable by fluorescence, it was not detected efficiently by IP; ...
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