EF, elongation factor
Ude, Susanne Caroline Margarethe (2013): The role of elongation factor EF-P in translation and in copy number control of the transcriptional regulator CadC in Escherichia coli. Dissertation, LMU München: Fakultät für Biologie
... translation elongation factor EF-P at a conserved lysine residue ...(K34). EF-P and its ortholog eukaryotic initiation factor 5A (eIF5A) have been investigated for more than thirty ...
140
Structural Basis for EarP Mediated Arginine Glycosylation of Translation Elongation Factor EF P
... ranslation elongation is a nonuniform process and directly depends on the amino acids (aa) to be incorporated into the growing polypeptide chain ...archaeal elongation factor 5A (e/aEF-5A) (5–7) and ...
20
Cloning, expression and functional study of translation elongation factor 2 (EF 2) in zebrafish
... translation elongation factor 2 (EF-2) in zebrafish (GenBank Accession ...zebrafish EF-2 shows that the 2826 bp cDNA spans an open reading frame between nucleotide 55 to 2631 and encodes a ...
5
Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae.
... Amino acid substitutions located adjacent to a putative aminoacyl-tRNA binding region also suppress frameshift and nonsense mutations.. These mutations may alter the bindi[r] ...
12
Purification and characterization of elongation factor 2 (EF2) and cyclic AMP-independent protein kinases from soybean (Glycine max, L) cotyledons.
... salt (0.5 H KC1) wash ribosomal fraction is very active in ADP-ribosy lation. Dissociation reagents like ammonium chloride and EDTA have been used in releasing bound EF2 (Smulson et al., 1970; Traugh and Collier, 1971; ...
155
Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance in Pseudomonas aeruginosa
... His-tagged purification of EarP and EF-P. XJB BL21(DE3) cells were used in all cases for recombinant protein expression. N-terminal His6 – EF-P was expressed in LB supplemented with 0.2% arabinose and 150 ...
9
Ribosomal Elongation Factor 4 Promotes Cell Death Associated with Lethal Stress
... when EF-G mal- functions ...with EF-G for binding to the PRE complex ...either EF-G or EF- Tu. Interfering with EF-G and/or EF-Tu function by EF4 may suppress tmRNA from rescuing ...
9
Maintenance of Transcription Translation Coupling by Elongation Factor P
... lation, EF-P can affect gene expression at the level of ...that EF-P restored coupling (Fig. 2). Sim- ilar effects of EF-P on coupling were found with the intrinsic ter- minator ...
11
Metabolizable energy, nitrogen balance, and ileal digestibility of amino acids in quality protein maize for pigs
... content of these amino acids. However, maize Opaque 2 had a soft endosperm that made it susceptible to pests and crop storage problems, for which its production was ceased. Subsequent conventional breeding efforts by the ...
10
Giardia secretome highlights secreted tenascins as a key component of pathogenesis
... 2; DIDS: 4,4 -disothiocyanatostibene-2,2 -sulfonic acid; DMEM: Dulbecco’s Modified Eagle Medium; EF-1 α : elongation factor 1-alpha; EGF: epidermal growth factor; FDR: false discovery [r] ...
13
The root of the universal tree and the origin of eukaryotes based on elongation factor phylogeny
... This rooting for the universal tree was also supported by high bootstrap values 91-99% with EF-Tu and 94-97% for EF-G when three-way analyses were performed using another member of the G[r] ...
7
Molecular Phylogeny and Proposal of Two New Species of the Emerging Pathogenic Fungus Saksenaea
... the elongation factor 1 ␣ (EF-1 ␣ ) gene, as well as by evaluation of relevant morphological and physiological characteristics of a set of clinical and environmental strains, we have demonstrated ...
7
Characterization of two genes involved in Neotyphodium lolii growth : a thesis presented in partial fulfillment of the requirements for the degree of MAster of Science in Biochemistry at Massey University, Palmerston North, New Zealand
... Three candidate genes were selected: Protein Elongation Factor 2 EF-2 Cell Division Control Protein 12 - a septin CDC-12 Stretch-Activated Calcium Channel SACC The EF-2 gene was selected[r] ...
221
Cellular Elongation Factor 1δ Is Modified in Cells Infected with Representative Alpha-, Beta-, or Gammaherpesviruses
... phosphorylates EF-1d should, in retrospect, have come as no surprise, since protein synthesis is the key to viral replication and yet the translation of mRNA seems immune to viral ...
5
Original Article A novel genotype screening and phylogenetic analysis of Blastocystis hominis based on EF-1α
... The elongation factor-1 alpha (EF-1α) is a highly conserved ubiquitous protein that is involved in translation and is desirable for use in phyloge- netic studies on Blastocystis, an enigmatic ...
10
Characterization of two genes involved in Neotyphodium lolii growth : a thesis presented in partial fulfillment of the requirements for the degree of MAster of Science in Biochemistry at Massey University, Palmerston North, New Zealand
... Protein elongation factor 2 EF-2; an elongation factor associated with the ribosome was targeted to add more direct evidence to the high metabolic rate observed in planta using the GUS r[r] ...
16
Avian Reovirus Nonstructural Protein p17-Induced G2/M Cell Cycle Arrest and Host Cellular Protein Translation Shutoff Involve Activation of p53-Dependent Pathways
... 4E-BP1 is reported to be phosphorylated when members of the PI3K-related kinase and protein kinase C families of pro- tein kinases are activated (18). Rates of translation of mRNAs encoding several cell cycle-related ...
13
Interaction of nuclear factor EF-1A with the polyomavirus enhancer region.
... EF-1A binding to Py site 1 is independent of cooperation with other EF-1A sites or the adjacent binding sites for PEA-1 and PEA-2, two murine nuclear factors that bind in the Py A enhanc[r] ...
9
Inhibition of elongation factor 1A-1 activity and hepatic lipotoxicity
... Elongation factor 1A-1 (eEF1A-1) was previously identified as a mediator of fatty acid-induced cell death (lipotoxicity) downstream of endoplasmic reticulum (ER) ...peptide elongation activity of ...
130
Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta.
... The strains with tefs::TRPl suppressed by extra copies of TEF2 were slow growing, cold sensitive, hypersensitive to inhibitors of translation elongation and showed i[r] ...
9